Avian infectious bronchitis virus attenuated virus strain rH120-YZ and construction method thereof
A rh120-yz, bronchitis technology, applied in the field of vaccine manufacturing, can solve the problems of genotype inconsistency, etc., and achieve the effects of low pathogenicity, high reproductive titer, and broad application prospects
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[0024] The following examples describe the present invention in detail. The examples are only intended to illustrate the present invention, but are not used to limit the scope of the present invention. The scope of the present invention is specifically defined by the appended claims.
[0025] Step 1: Cloning of the spike protein gene S of infectious bronchitis virus isolate
[0026] Preparation of biological materials:
[0027] The infectious bronchitis virus isolate ck / CH / IBYZ / 2011 was isolated, identified and preserved by the Institute of Poultry Disease Control, Jiangsu Poultry Science Institute. The applicant promises to release it to the public for 20 years from the date of application.
[0028] SPF chicken embryo: purchased from Beijing Meriya Weitong Laboratory Animal Technology Co., Ltd.
[0029] pMD18T vector: purchased from Dalian Bao Biological Engineering Co., Ltd.
[0030] JM109 genetically engineered bacteria: purchased from Dalian Bao Biological Engineering Co., Ltd.
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