Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Avian infectious bronchitis virus attenuated virus strain rH120-YZ and construction method thereof

A rh120-yz, bronchitis technology, applied in the field of vaccine manufacturing, can solve the problems of genotype inconsistency, etc., and achieve the effects of low pathogenicity, high reproductive titer, and broad application prospects

Inactive Publication Date: 2015-06-10
JIANGSU INST OF POULTRY SCI +1
View PDF3 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The first purpose of the present invention is to invent a weakened strain of chicken infectious bronchitis virus, thereby solving the problem that the genotypes of the currently used IB vaccine strains are inconsistent with those of the epidemic strains

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Avian infectious bronchitis virus attenuated virus strain rH120-YZ and construction method thereof
  • Avian infectious bronchitis virus attenuated virus strain rH120-YZ and construction method thereof
  • Avian infectious bronchitis virus attenuated virus strain rH120-YZ and construction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The following examples describe the present invention in detail, and the examples are only intended to illustrate the present invention, but are not intended to limit the scope of the present invention. The scope of the invention is specifically defined by the appended claims.

[0025] Step 1: Cloning of Spike Protein Gene S of Infectious Bronchitis Virus Isolate

[0026] Preparation of biological material:

[0027] Infectious bronchitis virus isolate ck / CH / IBYZ / 2011 was isolated, identified and preserved by the Institute of Poultry Disease Control, Jiangsu Institute of Poultry Science. The applicant commits to release to the public for 20 years from the filing date.

[0028] SPF chicken embryos: purchased from Beijing Merial Verton Experimental Animal Technology Co., Ltd.

[0029] pMD18T vector: purchased from Dalian Bao Biological Engineering Co., Ltd.

[0030] JM109 genetically engineered bacteria: purchased from Dalian Bao Biological Engineering Co., Ltd.

[...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an avian infectious bronchitis virus attenuated virus strain rH120-YZ and a construction method thereof. The microbial preservation number of the avian infectious bronchitis virus attenuated virus strain rH120-YZ is CGMCCNo. 8501. The invention relates to an application of a reverse genetic manipulation technology. By using a constructed reverse genetic manipulation platform of the avian infectious bronchitis virus attenuated virus strain rH120-YZ and adopting a spike protein gene S of an avian infectious bronchitis virus attenuated virus isolate ck / CH / IBYZ / 2011 to replace a corresponding part of the H120 vaccine strain genome, a recombinant virus rH120-YZ is saved and obtained. The virus has relatively low pathogenicity on chicks, has a relatively high breeding titer on chick embryos, is suitable for large-scaled production of infectious bronchitis vaccines and can be used for manufacturing vaccines.

Description

technical field [0001] The invention relates to applying reverse genetic manipulation technology to produce rH120-YZ attenuated strain of chicken infectious bronchitis virus for making vaccine. Background technique [0002] Infectious bronchitis (IB), one of the second-class infectious diseases of poultry stipulated by the International Veterinary Bureau, is an acute, highly contagious infectious disease caused by infectious bronchitis virus (IBV) , causing serious economic losses to the poultry industry, seriously restricting the development of the poultry industry in the world [Cavanagh D. Coronaviruses in poultry and other birds. Avian Pathol, 2005, 34(6): 439-448.]. Although vaccines play an important role in disease control, with the continuous emergence of different lesion types and tissue-tropic strains, vaccines developed by traditional methods are far from meeting the needs of IB prevention and control. Based on this, developing safe and efficient new vaccines has ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N7/01C12N15/09C12N15/50C12R1/93
Inventor 周生邹剑敏戴有理戴亚斌唐梦君陈连颐许明姜逸赵宝华程旭沈欣悦李建梅尤素兰
Owner JIANGSU INST OF POULTRY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com