80 results about "Bronchitides" patented technology

The invention discloses a multi-RPA primer and probe for simultaneously detecting a newcastle disease virus, an infectious bronchitis virus and an H9N2-subtype avian influenza virus. The multi-RPA primer and probe comprise a primer and probe for detecting the newcastle disease virus, a primer and probe for detecting the infectious bronchitis virus and a primer and probe for detecting the H9N2-subtype avian influenza virus. The multi-RPA primer and probe are high in specificity, high in sensitivity and accurate in detection result. The invention further discloses a method for simultaneously detecting the newcastle disease virus, the infectious bronchitis virus and the H9N2-subtype avian influenza virus through multi-RPA. The method comprises the steps of primer synthesis, template RNA extraction, reverse transcription, multi-PCR amplification and amplification product analysis. The detection method is simple in operation and good in stability, and a low-cost, fast and specific field diagnosis method is provided for effective detection and identification of mixed infection of common avian respiratory diseases.

The invention discloses an indirect enzyme-linked immuno sorbent assay (ELISA) kit for detecting avian infectious bronchitis virus (IBV) antibody. The kit contains an ELISA plate enveloped by IBV-N recombinant protein serving as antigen. The IBV-N recombinant protein is obtained by the following method: designing a pair of specific primers according to an IBV-N gene sequence; amplifying the N gene of IBV by using a reverse transcription polymerase chain reaction (RT-PCR) method, directionally inserting the N gene into a pET-32a(+) expression vector, and screening to obtain a positive recombinant expression plasmid of pET-32a(+)-IBV-N; and transferring the plasmid to a BL21 competent cell, and performing isopropyl thiogalactoside (ITPG) induction expression to obtain the IBV-N recombinant protein. The kit is low in cost, easy, convenient and quick to operate, and particularly suitable for detecting batch samples, and greatly improves the efficiency of serodiagnosis of the avian infectious bronchitis.

The present invention relates, in general, to the genetics of viruses. In particular, the present invention provides methods of distinguishing between serotypes of avian infectious bronchitis virus based on restriction fragment length polymorphisms derived from the region of the S1 gene of IBV, peptides derived from the restriction fragments, related primers for polymerase chain reaction, and certain restriction length polymorphism patterns.

The present invention provides materials and methods for researching poultry viruses, particularly for researching infectious bronchitis viruses in poultry. Also provided are materials and methods useful for reducing the economic impact that infectious bronchitis disease has on poultry production. In one aspect of the invention, there are provided nucleic acids, amino acids and related materials and compositions useful for combating infectious bronchitis virus in poultry.

The present invention relates to novel peptides that are highly biologically and pharmacologically active as therapeutic agents for the treatment of numerous lung diseases or lung and / or bronchi related diseases, especially chronic obstructive pulmonary disease (COPD), cystic fibrosis (CF), and Bronchiolitis obliterans (BO). The synthetic peptides according to the invention are derivatives of vasoactive intestinal peptide (VIP) and show enhanced physical, pharmacological and biological / therapeutic properties compared to VIP.

The invention relates to the field of tea, and particularly discloses health-care tea which comprises the following components in mass percent: 90 to 96 percent of old Yunnan cooked puer tea, 2 to 6 percent of old Xinhui dried orange peel and 2 to 4 percent of lily. In order to better implement the technical scheme of the invention, the invention also discloses a preparation method of the health-care tea, and the pharmacological active substance of the dried orange peel and the fragrance of the lily are adsorbed into the tea by mainly utilizing a scenting technology in the preparation method. The health-care tea of the invention not only has specific fragrance, but also has the efficacy of promoting digestion and warming the stomach, relieving sunstroke and expelling dampness, lowing lipid and relaxing the bowels, regulating qi and strengthening the spleen and the stomach, drying dampness and eliminating phlegm and the like and has specific curative effects for bronchitis patients, deficient-body groups and abundant-phlegm people.

The present invention belongs to the field of animal virology technology, and is especially one kind of short peptide capable of combining specifically with chicken infectious bronchitis virus (IBV) H52, bacteriophage containing the short peptide and its preparation process. The short peptide has the amino acid sequence of GSHHRHVHSPFV, and may combine with chicken infectious bronchitis virus H52 specifically after passing through the substitution, deletion or addition of one or several amino acid residues. The present invention also includes the application of the bacteriophage in preparing IBV identifying and diagnosing kit, and the IBV identifying and diagnosing kit is used in the preventing and treating of chicken IBV.

The invention discloses a process for preparing compound granule and the quality control method, wherein the compound granule is prepared from flitilarry bulb fluid extract, liquid extract of liquorice, platycodon glaucus, roor of sessile stemona, peucedanum root, pinellia tuber, dried orange peel, ammonium chloride, ephedrine hydrochloride and menthanol. The quality control method is characterized by the thin layer chromatogram authentication to dried orange peel, peucedanum root and licorice root, as well as the content measuring method for ephedrine hydrochloride.

The invention relates to an artificially weakened infectious bronchitis virus and application thereof. The artificially weakened infectious bronchitis virus uses the infectious bronchitis virus (IBV) which has good immunogenicity and is separated by an inventor to be passaged and attenuated, so as to obtain an attenuated strain FNO-E55 as a virus seed for production to prepare a safe and effective infectious bronchitis live vaccine. The strain used in the invention can effectively prevent occurrence of infectious bronchitis resulted from the infectious bronchitis virus (793 / B serotype) by aiming at a prevalence situation of the current domestic infectious bronchitis.

The invention provides an indirect ELISA (enzyme-linked immuno-sorbent assay) kit for detecting a nephropathogenic avian infectious bronchitis virus and an antibody thereof, and belongs to the technical field of biology. The indirect ELISA kit for detecting the nephropathogenic avian infectious bronchitis virus and the antibody thereof comprises an ELISA plate which is coated by DS10 monoclonal antibodies resisting the nephropathogenic avian infectious bronchitis virus and is combined with DS10 inactivated purified antigens resisting the nephropathogenic avian infectious bronchitis virus. The indirect kit for detecting the nephropathogenic avian infectious bronchitis virus and the antibody thereof has the advantages of specificity, sensitivity, quickness and simplicity and convenience in operation, and can be used for quickly diagnosing whether chickens are infected by the nephropathogenic avian infectious bronchitis virus or not and monitoring the immune antibody in actual production.

The invention discloses a kit for detecting infectious laryngotracheitis virus, newcastle disease virus and infectious bronchitis virus. The kit disclosed by the invention is capable of correctly and effectively detecting the infectious laryngotracheitis virus, the newcastle disease virus and the infectious bronchitis virus, and is strong in specificity, high in sensitivity, short in time, rapid in detection and good in application prospect.

The invention belongs to the filed of biotechnology and discloses a primer for detecting infectious bronchitis viruses, a detection method and a kit. The primer comprises the following components: a forward direction outer primer 1: N1-F2, a reverse direction outer primer 1: N1-B2, a forward direction outer primer 2: N1-F3, a reverse direction outer primer 2: N1-B3, a forward direction inner primer N1-F1P, a reverse direction inner primer N1-B1P, a forward direction loop primer N1-FIC, and a reverse direction loop primer N1-BIC. The invention further provides the method and the kit for detecting infectious bronchitis viruses. The detecting method and the kit have the characteristics of speediness, sensitiveness, simplicity, convenience, high efficiency, easiness in judgment and the like, and can make up the deficiencies of the existing method for detecting infectious bronchitis viruses.

The invention provides a universal kit for detecting different genotypes of infectious bronchitis viruses, and belongs to the technical field of RT-PCR detection. The kit contains a pair of specific primers, and the nucleotide sequences of the specific primers are shown as SEQ ID NO.1 and SEQ ID NO.2 respectively. The invention further provides a method for detecting the different genotypes of infectious bronchitis viruses. The kit and the detection method have the advantages of high specificity, high sensitivity, high efficiency, good universality and low cost and can be used for conducting rapid differential diagnosis on clinical disease materials in 6.5 h, and a technical means is provided for early rapid diagnosis on the IBVs and development of molecular epidemiological investigation to better guide prevention and control over the disease in poultry raising production.

The invention discloses a gene chip and a kit for infectious bronchitis viruses and / or infectious laryngotracheitis viruses. The gene chip and detection kit disclosed by the invention can accurately and effectively detect infectious bronchitis viruses and / or infectious laryngotracheitis viruses, and are strong in specificity, high in sensitivity, short in time consuming and rapid in detection, and has a good application prospect.

The production method of transgenic potato for expressing infectious bronchitis virus fibromutein includes the following steps: inserting the infections bronchitis virus fibromutein gene onto the plant expression victor pB1121, using triparental crossing method to introduce the recombinant expression victor into agrobacterium, using exogenous gene to transform the part of potato tuber by means of agrobacterium mediatization method, and utilizing antibiotic resistance of regenerated plant to screen and make molecular analysis by using PCR, Southern bolt and Northern bolt to obtain the transgenic plant expressing infectious bronchitis virus fibromutein. The transgenic plant can be used for feeding chicken or the infectious bronchitis virus fibromutein extracted and purified from said transgenic plant can be made into injection to immunize chichen.

The invention relates to a preparation method of a quality control standard substance of a novel coronavirus detection kit. According to the invention, the avian infectious bronchitis virus of the same family as the novel coronavirus (SARS-CoV-2) is used as the quality control standard substance of the novel coronavirus nucleic acid detection kit. At present, novel coronavirus detection kits are uneven in quality level, positive quality control is plasmid samples (DNA), and the virus reverse transcription process cannot be supervised and evaluated. Meanwhile, due to the diversity and complexity of clinical samples, the instability of a detection result is also caused. The quality control product provided by the invention can provide sensitivity and specificity evaluation support in the processes of novel coronavirus detection kit development, delivery inspection and preservation period monitoring; influences of various factors on the quality of the kit in the production process are effectively monitored, and meanwhile, the kit is used for evaluating the inactivation effect of a sample preservation solution on viruses, so that thorough inactivation of the viruses in a sample is ensured, effective preservation of viral nucleic acid is also ensured, the preservation time of the nucleic acid is prolonged, and the detection sensitivity is ensured.

The present invention provides avian infectious bronchitis virus IBV-K136, a monoclonal antibody cell line 3D5 prepared by using the avian infectious bronchitis virus IBV-K136, monoclonal antibodies, and applications of the avian infectious bronchitis virus IBV-K136 and the monoclonal antibodies. According to the present invention, the IBV-K136 strain is an endemic nephropathogenic infectious bronchitis virus strain and is obtained through screen, the IBV-K136 strain is adopted as antigen to amplify and then animal immunization is performed, the immune sera and myeloma cells are fused to screen hybridoma cells, the excellent performance avian infectious bronchitis virus monoclonal antibody cell line 3D5 is screened by using the method, and the cell line 3D5 is culture so as to express a lot of monoclonal antibodies; and the monoclonal antibodies prepared by using the process have characteristics of high monoclonal antibody titer, rapid antigen-antibody reaction, and strong specificity, and is especially suitable for further preparation of the avian infectious bronchitis virus antigen diagnosis kit, and the corresponding detection methods can be the double-antibody sandwich ELISA or colloidal gold method, and the like.

The invention discloses compound bronchitis syrup. The syrup is prepared from the following active pharmaceutical ingredients in parts by weight: 0.6-1.2 parts of carbetapentane citrate, 6-12 parts of ammonium chloride, 30-40 parts of almonds, 20-25 parts of gypsum, 20-45 parts of platycodon root, 3-9 parts of sodium benzoate, 70-90 parts of liquid extract of liquorice, 350-400 parts of sugars and the balance of water. The syrup has the good effects that the syrup combines the traditional Chinese medicines with the western medicine; the traditional Chinese medicines are used for treating the root causes and have the functions of enhancing the repairing and healing effects of bronchitic foci, enhancing the immunological functions of bodies and restoring the accommodation function of human bodies to the environment; the western medicine is used for treating the symptoms and has the functions of eliminating trachea and lung inflammation and improving the lung functions; the syrup absolutely conforms to the principles of the traditional Chinese medicines and the western medicine and the characteristics of minds and bodies of bronchitis patients; on the premise of the general principles, different compositions can be provided according to different people and have different functions; the medicine sources are accessible and the costs of the medicines are low, so that the burdens of the patients can be reduced.

The invention relates to a scutellaria baicalensis, manchurian lilac bark and dahurian rhododendron leaf cough-arresting mixture, which is prepared from active components, additives and water. The preparation method comprises the following steps of performing water extraction and alcohol precipitation on raw materials dahurian rhododendron leaf, manchurian lilac bark and scutellaria baicalensis; combining the dahurian rhododendron leaf alcohol precipitation extract and the manchurian lilac bark alcohol precipitation extract; refrigerating at low temperature; and adding the additives, wherein the alcohol precipitation step is performed before the combining step, and the low-temperature refrigerating step is performed before the additive adding step. The scutellaria baicalensis, manchurian lilac bark and dahurian rhododendron leaf cough-arresting mixture has the beneficial effects that an optimal proportion of scutellaria baicalensis, manchurian lilac bark and dahurian rhododendron leaf is disclosed by adopting the optimal alcohol precipitation process of dahurian rhododendron leaf and manchurian lilac bark and the low-temperature refrigeration step, and ethanol is not required to be added in the whole prescription for inhibiting bacteria, so that the best curative effects of an empirical formula formed by dahurian rhododendron leaf, manchurian lilac bark and scutellaria baicalensis is sufficiently played. The effective rate of the cough-arresting mixture to acute attack of chronic bronchitis is 97.93 percent along with remarkable curative effect and low side effect.

The invention discloses a detection kit for chicken infectious bronchitis indirect ELISA antibody, belonging to the field of biotechnology. According to the invention, genetically engineered bacterium capable of steadily expressing partial nucleoprotein of chicken infectious bronchitis virus is utilized for inducible expression of recombined N160 protein which is subjected to affinity chromatographic purification, and the purified recombined N160 protein is taken as a coating antigen, so as to establish a simple and convenient and specific indirect ELISA method. The invention further provides a specific, sensitive, quick and convenient-to-operate kit for detecting the antibody of the chicken infectious bronchitis virus, which can be used for quickly diagnosing whether the chicken flocks are infected by the chicken infectious bronchitis virus and monitoring the immune chicken serum antibody level and the chicken maternal antibody level in actual production, so as to evaluate the degree of risk that the chicken flocks are infected by the infectious bronchitis virus and provide reference for the formulation of an infectious bronchitis immune procedure.

The invention provides an avian infectious bronchitis virus IBYZ and an application thereof, and relates to the technical field of virology. The preservation number of the avian infectious bronchitisvirus IBYZ is CGMCC No. 14682. The avian infectious bronchitis virus IBYZ has typical coronavirus genome coding structure characteristics, and belongs to a predominant prevalent QX genotype at present. The strain can cause typical clinical symptoms of SPF chicks, the death rate of seven-day-old SPF chicks is 50% or above, a stable pathogenesis model can be established through the strain, and the strain can be used for researching the pathogenesis of the virus and evaluating the immune protection effect of IB vaccines.

The invention discloses a children's bronchitis syrup, which is prepared from the following raw materials by are weight: 0.8-1.2 parts of carbetapentane citrate, 6-9 parts of ammonium chloride, 45-60 parts of Berberis thunbergii, 62-80 parts of sunglo, 32-45 parts of Platycodon grandiflorum, 4-10 parts of sodium benzoate, 30-40 parts of an extractum glycyrrhizae liquidum 280-360 parts of carbohydrate and the balance of water. The invention has the following benefit effects: the syrup has definite therapeutic effect fast effectiveness and no toxic or side effect; the preparation method is easy and simple for production operation; the syrup is convenient for storage and transport; the medicinal herbs resources are easily available, so as to mitigate the burden of patient; and the syrup has sweet taste and small dosage, and is easily accepted by the children.

The invention relates to the technical field of virus detection, and particularly discloses a primer and a probe for detecting an infectious bronchitis virus as well as a detection method and application. In the primers and the probe for detecting the infectious bronchitis virus, the sequence of an upstream primer is shown as SEQ ID NO: 1, the sequence of a downstream primer is shown as SEQ ID NO: 2, and the sequence of the probe is shown as SEQ ID NO: 3. By utilizing the combination of the primer and the probe, the real-time fluorescence RAA detection of the infectious bronchitis virus can be realized, and the detection method has the advantages of high specificity, good sensitivity, accuracy and high efficiency.

The invention provides T cell antigen epitope polypeptide of avian infectious bronchitis virus N protein and application of the T cell antigen epitope polypeptide, and belongs to the technical field of epitope polypeptide vaccine preparation. A series of overlapped peptide fragments covering a full-length amino acid sequence are designed according to an N protein amino acid sequence of IBV, lymphocytes are respectively stimulated by the overlapped peptide fragments, the IFN-gamma secretion level of IBV specific T cells after being stimulated by the polypeptide is detected, and functional T cell epitope polypeptide is preliminarily screened out. The functional T cell epitope polypeptide is used for respectively stimulating and immunizing chicken spleen lymphocytes, and a peptide fragment capable of effectively inducing CD8 + T lymphocytes to secrete IFN-gamma is obtained through an IFN-gamma intracellular cytokine staining test. The obtained antigen epitope polypeptide has strong immunogenicity, can be used for preparing IBV multi-epitope vaccines, is high in safety and good in specificity, and has potential application value in IBV prevention and control work.

The invention provides an infectious bronchitis virus rH120-YZS1 delta 5a as well as a construction method and application thereof, and belongs to the technical field of molecular biology and gene engineering. The infectious bronchitis virus rH120-YZS1 delta 5a is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC No.10499. The infectious bronchitis virus rH120-YZS1 delta 5a can be adopted to express S1 protein of QX type and Mass type infectious bronchitis viruses at the same time, so that the infectious bronchitis virus rH120-YZS1 delta5a can be used for preparing bivalent vaccines for preventing infectious bronchitis.

The invention discloses an infectious bronchitis recombinant virus lack of E protein ion channel activity as well as a preparation method and application. A 26-site amino acid of a protein E of a wildinfectious bronchitis recombinant virus is mutated from aminopropionic acid to phenylalanine to obtain the infectious bronchitis recombinant virus lack of the E protein ion channel activity. By constructing the infectious bronchitis recombinant virus lack of the E protein ion channel activity and obtaining a mutant as well as by virtue of a separation purification method for the mutated virus andthe characteristic analysis of the mutated virus, the development of specific antiviral drugs and effective vaccines for preventing the virus infection can be facilitated, and the important public health and economic significance for researching corona virus can be achieved.