27 results about "Bronchitides" patented technology

The invention provides an infectious bronchitis virus subunit vaccine as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering vaccines. According to the chicken infectious bronchitis virus mutant protein disclosed by the invention, the amino acid sequence of the mutant protein is shown as SEQ ID NO.5. According to the avian infectious bronchitis virus subunit vaccine containing the recombinant avian infectious bronchitis virus mutant protein Kozak-IgKsp-IBV-M41-S-core-6His as an effective component, the glycosylation level of the vaccine is relatively high, the expression level is relatively stable, the virus return risk of a live vaccine does not exist, the safety is high, large-scale industrial preparation can be achieved, and the vaccine has good application prospects. The cost is low.

The present invention belongs to the field of biotechnology, and specifically relates to a primer-probe combination and a kit for simultaneously detecting IBV and its six genotypes. The primer-probe combination is for detecting GI-1 genotype of avian infectious bronchitis virus, for detecting GI-13 genotype of avian infectious bronchitis virus, for detecting GI-19 genotype of avian infectious bronchitis virus, for detecting GI-22 genotype of avian infectious bronchitis virus, for detecting GVI-1 genotype of avian infectious bronchitis virus, for detecting GI-7 genotype of avian infectious bronchitis virus or for detecting universal genotype of avian infectious bronchitis virus. The detection throughput of the present invention is high, and typing and universal detection of six genotypes can be completed in a single-tube reaction system, which is time-saving and efficient; the channel utilization rate is high, and each fluorescent channel supports typing of two genotypes, effectively expanding the multiplexing capability; the typing accuracy is strong, the typing signal is clear, and the accuracy rate is high.

The present application belongs to the field of veterinary drugs, and provides a recombinant protein S-Trimer of an infectious bronchitis virus antigen and a subunit vaccine therefor. Provided in the present application is a recombinant protein S-Trimer of an infectious bronchitis virus antigen, wherein the recombinant protein S-Trimer of the antigen has an amino acid sequence as set forth in SEQ ID No. 4. Also provided in the present application is an infectious bronchitis virus antigen. The infectious bronchitis virus antigen is of a trimer structure of the recombinant protein S-Trimer described above. The present application provides a subunit vaccine for an infectious bronchitis virus, wherein the subunit vaccine comprises a pharmaceutically acceptable carrier and an immunogenic amount of the infectious bronchitis virus antigen.

The invention provides modRNA (Ribonucleic Acid) for treating obliterated bronchitis, and belongs to the technical field of bioengineering. The modRNA for treating obliterated bronchitis provided by the invention is composed of an IL-10 modRNA (Interleukin-10) and an IFN (Interferon)-alpha modRNA (Interferon-alpha) modRNA. The BO treatment effect is achieved by inhibiting bronchial epithelial cell epithelial-mesenchymal transition (EMT), the expression of alpha-SMA and Vimentin in lung tissue can be remarkably reduced, the expression of E-cadherin and Cytokeratin 5 can be up-regulated, microbronchial epithelial injury is repaired, collagen deposition is reduced, and the lung function is improved. The synergistic effect of the two is better than that of single gene therapy, the safety is high, and the controllability is strong.

The application provides a general avian infectious bronchitis virus whole genome deep sequencing method and application. The method realizes efficient amplification of long fragments of whole genomes of different genotype IBV epidemic strains by designing and applying a set of combinations containing 24 pairs of specific primers. The specific sequences of the primer combinations are shown in SEQ NO:1 to SEQ NO:48. Compared with a traditional sequencing method, the application significantly reduces the sequencing cost. Only two PCR reactions are needed, and more than 27 kb of IBV full-length genome sequence can be successfully obtained, which provides a more economical and efficient solution for IBV related scientific research and molecular epidemiology monitoring, and helps to promote the development of related field scientific research.

The invention belongs to the technical field of research and development of quintuplet vaccines, and particularly relates to a chicken Newcastle disease virus strain LaSota, a chicken Newcastle disease virus strain H120, a chicken Newcastle disease virus strain N49, a chicken Newcastle disease virus strain FADV-4 and a chicken Newcastle disease virus strain HN01. The chicken Newcastle disease virus strain HN01 and the chicken Newcastle disease virus strain HN01 and the chicken Newcastle disease virus strain HN01 and the chicken Newcastle disease virus strain FADV-4 and the chicken Newcastle disease virus strain HN01 and the chicken Newcastle disease virus strain FADV-4 and the chicken Newcastle disease virus strain HN01 are combined. The chicken Newcastle disease virus LaSota strain is proliferated in chicken embryo fibroblasts or chicken kidney cells, and the chicken Newcastle disease virus LaSota strain and the preparation method thereof have the beneficial effects that the chicken Newcastle disease virus LaSota strain and the preparation method thereof can be used for preparing the chicken Newcastle disease virus LaSota strain. The preparation method comprises the following steps: selecting an inactivated newcastle disease virus LaSota strain, an inactivated bronchitis H120 strain, an inactivated bursal disease N49 strain, an inactivated fowl adenovirus FADV-4 strain and an inactivated mycoplasma synovium HN01 strain; it can be guaranteed that a follow-up vaccine has the advantages of being low in toxicity, high in biological safety, high in cross protection capacity, high in immune response speed, long in antibody protection period, high in cellular immune level and high in virus culture content.

The invention provides a diaminopyrimidine compound with a structure as shown in a formula (I), or a stereoisomer thereof, or a pharmaceutically acceptable salt thereof, or a solvate thereof, or a prodrug molecule thereof, or a deuteride thereof, or a tritium thereof, and an application of the diaminopyrimidine compound, or the stereoisomer thereof, or the pharmaceutically acceptable salt thereof, or the solvate thereof, or the prodrug molecule thereof, or the deuteride thereof, or the tritium thereof. The diaminopyrimidine compound provided by the invention has obvious inhibitory activity on RSV virus, has a chemical structure type completely different from that of the existing anti-RSV drug ribavirin and the like, is expected to be developed into a novel anti-RSV drug, is used for inhibiting replication or reproduction of the RSV virus, and has broad application prospects. The pharmaceutical composition can be used for preventing, relieving and / or treating respiratory syncytial virus infection and cytopathic effects caused by the respiratory syncytial virus infection, and can be used for treating bronchitis or pneumonia and other diseases caused by the respiratory syncytial virus infection.

The invention relates to the field of preparation of poultry vaccines, in particular to a preparation method of a chicken newcastle disease and avian influenza combined vaccine, which comprises the following steps: respectively inoculating susceptible chick embryos with Newcastle disease virus, infectious bronchitis virus and avian influenza virus, and harvesting infected chick embryo liquid to obtain first virus liquid, second virus liquid and third virus liquid; inoculating the susceptible duck embryo with the egg drop syndrome virus, and harvesting infected duck embryo liquid to obtain a fourth antigen; and concentrating the harvested first virus liquid, second virus liquid, third virus liquid and fourth antigen, inactivating, and mixing and emulsifying with an adjuvant. The vaccine prepared by the invention does not generate any adverse reaction to chickens, is high in experimental safety, can induce high-level neutralizing antibodies in immunized chickens, and can effectively reduce the incidence of Newcastle disease virus, infectious bronchitis virus, egg drop syndrome and avian influenza.

The present invention refers in general to the field of veterinary medicine and especially to the development of vectored vaccines using the Gallid alphaherpesvirus 3 (GaHV-3) or Marek's disease serotype 2 (MDV-2) vector, which contains protective antigens against different avian pathogens such as Newcastle disease virus (NDV), avian infectious laryngotracheitis virus (ILTV), infectious bursal disease virus (IBDV) or Gumboro disease virus, avian influenza virus (AIV), infectious bronchitis virus (IBV).

This invention discloses a chicken infectious bronchitis virus (IBBV) nanoparticle, its preparation method, and its application, belonging to the field of poultry vaccine technology. The nanoparticles are formed by the self-assembly of an SRBD-SpyTag fusion protein and a Ferritin-SpyCatcher fusion protein via a SpyTag / SpyCatcher covalent linkage system. The SRBD-SpyTag fusion protein is formed by fusing a SpyTag sequence to the N-terminus or C-terminus of the S1 protein receptor-binding domain of IBV. The nanovaccine prepared from these nanoparticles exhibits high safety, rapid antigen module replacement, and is suitable for preventing IBV infection. The technical solution of this invention has advantages such as structural stability, strong immunogenicity, and rapid iteration, providing a novel vaccine solution for poultry disease control.

The present disclosure relates to a three-dimensional structure including an inverted co-culture having an interior layer comprising a plurality of cells of a first cell type; an opposing exterior layer comprising a plurality cells of a second cell type; and a basement membrane matrix positioned between the interior layer and the exterior layer, wherein the three-dimensional structure has a diameter, and wherein said diameter is capable of being altered by an alloimmune reaction that disrupts the plurality of cells of the second cell type and leads to contraction of the plurality of cells of the second cell type. Also disclosed is a method of modeling Bronchiolitis Obliterans Syndrome, a method for assessing presence of or risk of developing a physiological condition, a method of identifying one or more biomarkers of a disease, and a method of a method of making a three-dimensional structure.

A bacteria-based compound for the prevention and treatment of a number of strains of avian influenza via oral intake by way of feed or drinking water or both. The agent is an inventive treatment compound comprising a bacterial-based culture which is directed to the prevention and treatment of avian influenza primarily for use in commercial birds such as poultry. The inventive treatment compound disclosed herein is produced from a lipopolysaccharide of a Gram-negative bacterial strain of the Brevundimonas group. The compound may be delivered to poultry by way of liquid or dry feed according to an effective prescribed regimen. A similar treatment approach may be taken as well after the bird is infected. Use of the composition is effective in the prevention and treatment of a variety of avian influenza, including high pathogenicity avian influenza, low pathogenicity avian influenza, Newcastle disease virus, infectious bronchitis disease virus, and infectious laryngotracheitis virus.

This invention relates to the field of biotechnology, specifically disclosing a detection element, kit, and detection method for detecting infectious bronchitis virus (IBV) in chickens. The detection element is a Toehold Switch element, which is an S4-3 element and / or an S3 element; the nucleotide sequence of the S4-3 element is shown in SEQ ID NO: 1; the nucleotide sequence of the S3 element is shown in SEQ ID NO: 2. The detection element of this invention features high sensitivity and strong specificity, enabling the differentiation of three IBV strains—P65, MH20, and H120—at a single-base resolution.

The invention provides a primer and probe composition for typing detection of an infectious bronchitis virus. The composition is used for detecting genotypes of 4 / 91, TC07-2, LDT3, QX, LSC-99I and TW. The invention also provides a detection kit containing the primer and probe composition and a detection method. According to the invention, the detection of six genotypes is integrated into only two reaction tubes, the reaction steps are obviously reduced, the flux is improved, and a great leap is realized in multiple detection capability and detection efficiency, so that the kit is suitable for large-scale clinical screening and epidemiological investigation. The invention provides an unprecedented convenient and accurate tool for the diagnosis of complex clinical mixed infection by effectively identifying and combining the mixed infection of a plurality of genotypes through one-time reaction.

Provided herein are non-naturally occurring, broadly reactive antigens derived from influenza viruses or avian infectious bronchitis virus (IBV) that are immunogenic and capable of eliciting a broadly reactive immune response, e.g., a broadly reactive neutralizing antibody response, directed against influenza virus antigens or IBV antigens following introduction into a subject. Also provided are non-naturally, broadly reactive immunogens, vaccines, virus-like particles (VLPs) and compositions comprising the immunogens and vaccines. Methods of generating an immune response in a human or non-human subject by administering the immunogens, vaccines, VLPs, or compositions thereof are provided. In particular, the immunogens comprise broadly reactive hemagglutinin (HA) protein antigens of influenza virus strains, such as H1, H2, H3, H5, or H7, or of IBV. The immunogens also comprise broadly reactive viral neuraminidase (NA) protein antigens.

The invention discloses a chicken infectious bronchitis prediction method based on an AI chip, and relates to the technical field of animal epidemic disease intelligent monitoring and prediction, and the method comprises the following steps: S001, building a nanosecond-level unified time reference, continuously collecting chicken house environment sound signals through a distributed acoustic collection unit, generating a phase and energy joint spectrum according to the time reference, and carrying out the detection of the phase and energy joint spectrum; and stripping fundamental tone and harmonic components of low-frequency mechanical vibration by using a spectral difference operator to form a dynamic reference signal with separated sound sources. According to the method, the acoustic processing link is driven through the nanosecond time base, dynamic extraction and reconstruction of cough sound are achieved, a false alarm path is recognized in combination with residual density and confidence gating, an interference field is constructed through inverse sound wave injection, the recognition threshold value is dynamically adjusted, and the prediction precision and stability of an AI chip in a high-noise environment are improved.

An isolated infectious bronchitis (IB) virus (IBV) QX1285 strain under accession number DPS RE RSCIC 41 in the IZSLER biobank. A vaccine comprising the isolated infectious bronchitis virus and a carrier. The virus may be inactivated. The virus may alternatively be live and attenuated. The vaccine may be formulated for an avian. The avian may be a chicken. The vaccine may be administrated via drinking water. Also claimed is an isolated infectious bronchitis virus having an S1 protein encoded by SEQ ID NO: 1 or a sequence having at least 96% sequence identity thereto, for use as a vaccine against infectious bronchitis. Also claimed is a method of vaccinating an avian against infectious bronchitis, comprising administering the vaccine. Also claimed is a process for preparing a live infectious bronchitis vaccine comprising passaging the virus to reduce pathogenicity, harvesting the attenuated virus, and processing the virus to produce a vaccine. The virus may be passaged at least 50 times. The virus may be passaged by culturing in allantoic fluid of chicken embryos.

The invention provides a QX-type avian infectious bronchitis virus recombinant attenuated strain as well as a preparation method and application thereof, belongs to the technical field of veterinary biological products, and can solve the problems of long period and poor adaptability in acquisition of attenuated strains through multiple passage of chick embryos. According to the QX type avian infectious bronchitis virus recombinant attenuated strain, cDNA of a QX type avian infectious bronchitis virus serves as a template, after PCR amplification is conducted, infectious clone plasmids containing a QX type avian infectious bronchitis virus whole genome are constructed through a yeast homologous recombination technology, and the infectious clone plasmids are subjected to a PCR amplification to obtain the QX type avian infectious bronchitis virus recombinant attenuated strain. And co-transfecting a host cell with a recombinant plasmid carrying the QX type avian infectious bronchitis virus N protein gene and a 3'untranslated region, and then carrying out genetic rescue. The QX type avian infectious bronchitis virus recombinant attenuated strain disclosed by the invention is good in in-vitro proliferation characteristic and stable in genome heredity; the feed additive is good in safety and has no pathogenic risk to SPF chicken; the immune protection efficacy is prominent, and chicken flocks can obtain 100% protection against QX virulent virus attack after being immunized for 14 days; the method can be used for preparing vaccines.

The invention relates to a bimonoclonal antibody for broad-spectrum detection of an infectious bronchitis virus (IBV), an ELISA kit and application thereof, and aims to solve the problems of insufficient broad spectrum, poor specificity, complex operation and long time consumption of the existing IBV detection technology. According to the invention, double monoclonal antibodies (14C4 and 6B9) targeting different epitopes are adopted to synergistically coat a solid phase carrier and an enzyme label, and key parameters such as a coating solution, a diluent, a sealing condition and reaction time are optimized, so that a sandwich detection system capable of identifying Mass type, QX type, 793 / B type and GI-28 type strains is formed; according to the present invention, the rapid (1.5 h), specific, sensitive (the lowest detection limit of the viral genome is 3.168 copies / [mu] L, and the recombinant N protein is 3.906 ng / mL) and stable IBV antigen detection is achieved, the coincidence rate with qPCR is more than 96%, and the method is suitable for base layer and large-scale screening.

The application discloses Clostridium butyricum and application thereof, the Clostridium butyricum is isolated from the fresh content of the intestine of chicken resistant to IBV infection, has the effect of resisting broad-spectrum avian infectious bronchitis virus infection, has good protective efficacy to the infection of avian infectious bronchitis virus QX type strain and Mass genotype strain, and simultaneously, the application shows through experiments that the isolated Clostridium butyricum can activate chicken I-type interferon expression, realizes good protection effect to the infection of different serotypes of strains by improving I-type interferon expression.The Clostridium butyricum of the application solves the problem of low cross-protection efficacy of vaccine, greatly shortens the response time when the breeding industry encounters sudden IBV disease outbreak, and simultaneously, IBV prevention and control can be carried out by adding in drinking water, so that the use convenience is greatly improved, and the prevention and control work efficiency is increased.

The present application relates to the technical field of auscultation equipment, and discloses a wearable closed bronchiolitis auscultation detector, which comprises a binding belt and a display controller, further comprises a detector fixedly connected with the binding belt, a clamping sleeve clamped above the detector, four sound collectors arranged on the outer side of the detector, a receiver for collecting connecting lines, a splicing sleeve comprising a set position, when the splicing sleeve is not located at the set position, the two sides of the splicing sleeve are slidingly connected in the interior of the slot shell, when the splicing sleeve is located at the set position, the receiver adjusts the length of the connecting line through the splicing sleeve and the adjusting piece. Through line dynamic adjustment, the rotator of the receiver automatically collects and releases the connecting line with the activity of children, the sleeve block is slidingly arranged outside the straight rod, so that line winding or breakage is avoided, when the splicing sleeve slides to the set position, the sleeve block is connected with the arc block, the length of the line is accurately fine-adjusted through the adjusting piece, different children's chest circumferences are adapted, the skin is neither tightly compressed nor relaxed to cause the displacement of the collector.

The invention discloses a SOCS1 antagonistic peptide for preventing and treating avian infectious bronchitis and application of the SOCS1 antagonistic peptide, the amino acid sequence of the SOCS1 antagonistic peptide is LPQDKEYYKVKEP, and Y is phosphorylated tyrosine. Reasonable design is carried out on the basis of a molecular mechanism of interaction of a JAK2 activation ring and the SOCS1 protein, a molecular docking result shows that the SOCS1 antagonistic peptide can be precisely combined to KIR and SH2 functional domains of the chicken SOCS1 protein through a specific phosphorylated tyrosine residue of the SOCS1 antagonistic peptide, and the combination can competitively block combination of SOCS1 and endogenous JAK2 of cells, so that the chicken SOCS1 antagonistic peptide can be used for preparing the chicken SOCS1 antagonistic peptide. Therefore, the inhibition effect of SOCS1 on a JAK-STAT signal channel is relieved, the I-type interferon antiviral immune response of a host is recovered, and virus replication is inhibited fundamentally.

This invention discloses a duck-derived recombinant infectious bronchitis virus and its applications, belonging to the field of viral isolation and application. The virus was deposited at the China Center for Type Culture Collection on December 26, 2024, with accession number CCTCC NO: V202503. The duck-derived recombinant infectious bronchitis virus of this invention is capable of infecting CEF and DEF cells, is convenient for in vitro culture, and is suitable for the production of high-quality inactivated vaccines with high antigen titers. The duck-derived recombinant infectious bronchitis virus DIBVSD2401 of this invention has excellent immunogenicity, and the inactivated vaccine prepared using the duck-derived recombinant infectious bronchitis virus DIBVSD2401 of this invention has good safety and excellent protective effect.

This invention relates to the field of biomedical technology, specifically to the application of forsythoside in the preparation of drugs for the prevention and treatment of infectious bronchitis in chickens. Immunofluorescence and Western blotting results show that forsythoside can significantly inhibit the expression of viral nucleocapsid proteins in CEK cells and reduce the viral load in CEK cells. Furthermore, forsythoside can protect against IBV infection in broiler chickens. Quantitative fluorescence results indicate a significant reduction in viral replication in the tracheal tissue of broilers. Clinical symptoms and histopathological results show that forsythoside can significantly alleviate IBV infection-induced slow weight gain, respiratory distress, and tracheal tissue damage in broilers. This invention demonstrates that forsythoside has low cytotoxicity, can effectively prevent infectious bronchitis in chickens, and has advantages such as being green and safe, leaving no residue, having few toxic side effects, and being unlikely to induce drug resistance.

The invention relates to the technical field of auscultation equipment, and discloses a wearable occlusive bronchiolitis auscultation detector which comprises a bandage and a display controller and further comprises a detector fixedly connected with the bandage, a clamping sleeve connected to the upper portion of the detector in a clamped mode, four sound collectors located on the outer side of the detector and a storage device used for collecting connecting wires. The splicing sleeve comprises a set position, when the splicing sleeve is not located at the set position, the two sides of the splicing sleeve are slidably connected to the interior of the notch shell, and when the splicing sleeve is located at the set position, the storage device adjusts the length of the connecting line through the splicing sleeve and the adjusting piece. The rotator of the storage device automatically gathers and releases the connecting line along with the activity of the child through dynamic adjustment of the line, the sleeve block slidably sleeves the straight rod to avoid winding or breakage of the line, when the splicing sleeve slides to a set position, the sleeve block is connected with the arc-shaped block, the length of the line is accurately and finely adjusted through the adjusting piece to adapt to different chest perimeters of the child, and the skin is not tightly pressed; and the displacement of the collector caused by looseness is avoided.

The invention relates to the field of animal health. The present invention provides an attenuated infectious bronchitis virus (IBV). The attenuated infectious bronchitis virus can protect chickens from IBV infection.

The invention provides a general type avian infectious bronchitis virus whole genome deep sequencing method and application, and the method realizes efficient amplification of whole genome long fragments of IBV epidemic strains with different genotypes by designing and applying a set of combination containing 24 pairs of specific primers. The specific sequences of the primer combination are shown as SEQ NO: 1 to SEQ NO: 48. Compared with a traditional sequencing method, the method has the advantage that the sequencing cost is remarkably reduced. According to the method, the IBV full-length genome sequence exceeding 27 kb can be successfully obtained only through two PCR reactions, a more economical and efficient solution is provided for IBV related scientific research and molecular epidemiological monitoring, and scientific research and development in related fields are promoted.