A recombinant baculovirus, a preparing method thereof and applications in preparation of cancer vaccines

Abstract

The invention provides a bombyx mori recombinant baculovirus Bm-CMV-DLL4-DAF. The recombinant baculovirus adopts a recombinant baculovirus expression vector Pfastbacdual as a vector, and comprises a CMV-IE promoter, a DLL4 gene, a gp64 signal peptide gene, a DAF protein gene and a gp64 transmembrane domain gene. Preparation of the recombinant baculovirus includes steps of: subjecting the CMV-IE promoter sequence, the DLL4 protein bases, the gp64 signal peptide gene sequence, the gp64 transmembrane domain gene sequence and the DAF gene sequence to PCR amplification, constructing a Pfastbacdual-CMV-DLL4-DAF recombinant plasmid, and extracting viral genome. The recombinant baculovirus is good in stability, high in host infection rate, high in expression capability and capable of efficiently expressing DLL4 protein in mammal animal cells, thus provoking high and effective immunoreactions. The recombinant baculovirus is suitable for application in preparation of cancer vaccines.

Description

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AI Technical Summary

Benefits of technology

In this patented technical effect, the inventive technique described involves introducing an enzyme called cytoskeleton associated proteins (CNPs), like dikringin or kazalan, into live vector particles containing genes encoding various components involved in regulating different aspects of their function. These engineered molecules have improved properties compared to traditional methods mentioned earlier. They also enhance safety when administered safely while being less harmful due to its smaller size and reduced production costs. Additionally, they may provide better results because they help deliver drugs specifically towards targets within tumours rather than just inside them themselves. Overall, this process allows us to develop novel treatments based upon existing techniques alone.

Problems solved by technology

Technological Problem: Current Cancers Treatments (Clinics) Targeted Oncogene Pathways Layer 3 Ligase Signaling Pathway (LDPs), which are involved in controlling various aspects of biology including regulating different functions like cell division and proliferation. While some treatments aim at killing cells specifically targets these molecules without causing harm themselves, others use other methods to activate them effectively while avoiding their own effects. Therefore, finding ways to improve upon how they work may be crucial because current therapies often result in poorer outcomes than desired.

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