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Method for testing content of monosaccharide in polysaccharide in pear peel tissue

A method of determination and peeling technology, which is applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve the problems of less and less extraction of crude polysaccharides, and achieve the effect of improving the extraction rate, good repeatability, and high accuracy

Inactive Publication Date: 2015-06-17
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

At present, the determination of monosaccharide components in sample polysaccharides requires more test materials. If there are fewer samples, the amount of extracted crude polysaccharides will be less and subsequent work cannot be carried out.

Method used

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  • Method for testing content of monosaccharide in polysaccharide in pear peel tissue
  • Method for testing content of monosaccharide in polysaccharide in pear peel tissue

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Experimental program
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Embodiment 1

[0023] 1 Instruments and samples

[0024] 1.1 Instrument: Agilent 1260 HPLC system (Agilent Technologies, USA). Chromatographic column: ZORBAX SB-C18 column (4.6m*150mm*5μm, Agilent, USA).

[0025] Sample: the pericarp of 'Dangshansu pear' (green pear) and its brown-skinned buds changed to 'rustsu' (brown-skinned pear) 25d, 50d, 75d, 100d, 125d and 150d after flowering.

[0026] 2 Methods and results

[0027] 2.1 Weigh 2g of pear peels in different periods, extract with 6 times the volume of petroleum ether at 70°C for 2 hours, filter, collect the filter residue, heat the residue with 10 times the volume of deionized water for 3 times, the temperature of the water bath is 80°C, and the extraction time for each time 2h. The extract was centrifuged, and the supernatants were combined for 3 times, then concentrated in a rotary evaporator at 50°C under reduced pressure to 1 / 3 of the initial volume, added 4 times the volume of the concentrated solution in absolute ethanol, mixed...

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Abstract

The invention relates to a method for testing the content of monosaccharide in polysaccharide in pear peel tissue. The method comprises the following steps: preparing a sample to be tested, namely, weighing a fixed amount of pear peel, extracting with petroleum ether, performing heating extraction to the residues by using deionized water, filtering by using a membrane of 0.45mu m, putting the filtrate into an ampoule bottle, adding trifluoroacetic acid, introducing N2, sealing the tube to react for 0.5-5 hours, adding a NaOH solution and a PMP methanol solution, and uniformly mixing to react; preparing a standard monosaccharide solution, and quantitatively analyzing the content of different monosaccharide components in polysaccharide in the pear peel by using a high performance liquid chromatography method. The change rules of the content of the different monosaccharide components in polysaccharide in the peel in different periods of fruits can be compared. The method is small in sample amount, capable of effectively increasing the extraction rate of the content of the monosaccharide components in polysaccharide, and high in accuracy and repeatability.

Description

technical field [0001] The invention relates to a method for measuring sugar content, in particular to a method for measuring monosaccharide content in polysaccharides in pear peel tissue. Background technique [0002] At present, the methods and technologies for the determination of monosaccharide components in plant polysaccharides are mainly concentrated on the pulp of angelica, cactus, wolfberry, tremella and longan, but there are few methods for the determination of monosaccharide content in polysaccharides in pericarp tissue. Moreover, these methods require a large amount of samples, cumbersome steps and many sample raw materials, and it takes time and labor to obtain the pericarp samples produced at the young fruit stage of pears. Difficulty arises from the regularity of changes in the fruit and its role in the structure and function of the pericarp. [0003] Pears are the third largest fruit industry in my country and one of the most popular fruits for consumers. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/88
CPCG01N30/02
Inventor 衡伟朱立武程召阳叶振风贾兵刘莉刘普
Owner ANHUI AGRICULTURAL UNIVERSITY
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