Normal temperature preservation solution and preservation method of ovary

Abstract

The invention relates to ovary storing liquid and an ovary storing method, in particular to ovary constant-temperature storing liquid and an ovary constant-temperature storing method. The problems that at present, constant-temperature ovary storing time is over short, and the quantity and the quality of oocytes are affected are solved. The ovary constant-temperature storing liquid comprises phosphate buffer, glucose and tea polyphenols. The ovary constant-temperature storing method comprises the following steps of (1) removing adipose tissues surrounding an ovary and then rinsing the ovary by using normal saline; and (2) placing the ovary which is cleaned in the step (1) in the ovary constant-temperature storing liquid. The ovary constant-temperature storing liquid has the advantages of high efficiency, low cost, high convenience and zero toxicity on cells. The ovary constant-temperature storing method is simple and is easy to operate; other refrigerating equipment is not required; and the ovary constant-temperature storing liquid and the ovary constant-temperature storing method are particularly suitable for collecting ovaries of wild animals in fields; and the collected ovaries of the animals can be conveyed at constant temperature by a long distance.

Description

technical field

[0001] The invention relates to an ovary preservation solution and an ovary preservation method. Background technique

[0002] With the in-depth research of reproductive biotechnology (such as embryo engineering technologies such as nuclear transfer and transgenic), as well as the development of embryo production in vitro and commercialization of embryo transfer, how to obtain a larger number of high-quality oocytes has become a top priority. The collection and transportation of ovaries generally takes a long time. For example, when wild animal ovaries are obtained from the wild, female animal germ cells are transported between cities or internationally, and livestock ovaries are obtained from slaughterhouses, etc., the ovaries will be placed outside the body for a long time, resulting in the loss of oocytes. Decreased cell development.

[0003] Although the in vitro production of embryos by cryopreservation of ovaries has been successful, the maturation rat...

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