A kind of Pseudomonas putida p.pao‑1 with phosphorus removal function and its domestication and cultivation method

A technology for domestication and cultivation of Pseudomonas putida, which is applied in the methods of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., and can solve problems such as limiting the application of phosphorus accumulating bacteria and the effect of phosphorus removal strains on phosphorus removal ability.

Inactive Publication Date: 2017-06-30
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long proliferating process of bacteria needs to be accompanied by a high concentration of carbon source and a long time of microorganism / wastewater contact time, which is difficult to achieve in actual wastewater treatment projects. Application of Phosphorus Removal in Wastewater with Low Organic Concentration
However, if the traditional culture mode is changed, the enrichment-acclimation two-stage culture mode is adopted to cultivate the strains, that is, the first stage adopts enrichment culture, and the bacteria amount is amplified under the condition of high carbon source and nutritional balance, and the second stage adopts the condition of nutritional imbalance. Domestication and cultivation of the bacteria will induce the pre-synthesis and accumulation of PHB in the bacteria [5] , and a large amount of PHB pre-reserve in the bacteria will have a greater impact on the phosphorus removal ability of potential phosphorus removal strains

Method used

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  • A kind of Pseudomonas putida p.pao‑1 with phosphorus removal function and its domestication and cultivation method
  • A kind of Pseudomonas putida p.pao‑1 with phosphorus removal function and its domestication and cultivation method
  • A kind of Pseudomonas putida p.pao‑1 with phosphorus removal function and its domestication and cultivation method

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Experimental program
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Effect test

Embodiment 1

[0031] Pseudomonas putida strain P.PAO-1 forms a large amount of PHB primary accumulation in vivo and its ability to remove phosphorus under aerobic conditions:

[0032] Pseudomonas putida strain P.PAO-1 (preserved on August 26, 2013 in the General Microbiology Center of China Committee for Microorganism Culture Collection (CGMCC for short), the preservation number is CGMCC No.8073, the same below) was inoculated in 5ml sterilized enrichment medium (each L contains 3g beef extract, 10g peptone, 5g NaCl, 0.02g KH 2 PO 4 , Trace elements: each L contains 5g EDTA, 5gFeSO 4 ·7H 2 O, 1.6gCuSO 4 ·5H 2 O, 5g MnCl 2 4H 2 O, 50mg (NH 4 ) 6 Mo 7 o 24 4H 2 O, 50mgH 3 BO 3 , 10mgKI and 50mg CoCl 2 ·6H 2 (2, the same below) pre-cultured, and then inoculated into ten 100mL sterilized enrichment medium (250ml sterilized Erlenmeyer flask), respectively, at 30°C, 120r min -1 Under the conditions of shaking culture for 24 hours, washed with normal saline for 3 times, centrifuged...

Embodiment 2

[0038] Effect of adding Pseudomonas putida strain P.PAO-1 on the phosphorus removal ability of activated sludge after domestication culture

[0039] Under the same culture conditions, equal amounts of domesticated Pseudomonas putida strain P.PAO-1, ​​activated sludge, and activated sludge + domesticated Pseudomonas putida strain P. PAO-1 (the addition ratio is 1:1) is used for anaerobic / aerobic alternate culture, in which anaerobic 5h, aerobic 3h, respectively take 5ml of bacterial liquid at the end of anaerobic culture and during aerobic culture, centrifuge, and measure the bacteria Phosphorus release, phosphorus uptake and COD in the centrifugate.

[0040] The activated sludge collected from the return sludge well of the sewage plant was used for the addition experiment of Pseudomonas putida strain P.PAO-1. Since the sewage plant adopts the anoxic / anaerobic / aerobic (A / A / O) activated sludge process, the sludge contains a large number of active phosphorus-accumulating bacteri...

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Abstract

The present invention relates to a Pseudomonas putida P.PAO-1 with the function of removing phosphorus and a method for acclimating it. The preservation number of the Pseudomonas P.PAO-1 is: CGMCC No.8073, nucleoside The acid sequence is shown in SEQ ID NO.1. Cultivation: Inoculate Pseudomonas putida P.PAO-1 strains into 5-10ml sterilized enrichment medium for pre-culture, and then inoculate into 100-200ml sterilized enrichment medium, at 30°C , shake culture for 12‑24 hours, wash, centrifuge, and resuspend to form a bacterial liquid; then carry out anaerobic and aerobic alternate cultivation and domestication to obtain domesticated bacteria. The present invention adopts enrichment-domestication two-stage culture mode culture to form initial accumulation of polyhydroxybutyric acid in the P.PAO-1 strain, and is applied to the treatment of low-concentration carbon-containing organic wastewater to achieve the purpose of efficient phosphorus removal.

Description

technical field [0001] The invention belongs to the field of phosphorus-removing functional bacteria and a domestication and cultivation method thereof, and in particular relates to a Pseudomonas putida P.PAO-1 with phosphorus removal function and a domestication and cultivation method thereof. Background technique [0002] In recent years, the eutrophication of water bodies in my country has become increasingly serious; studies have shown that excessive discharge of phosphorus in sewage from a large number of urban sewage treatment plants is an important cause of eutrophication in water bodies [1] . In the biological treatment process for nitrogen and phosphorus removal adopted by sewage treatment plants in my country at this stage, the low biological phosphorus removal ability of activated sludge is an important problem to be solved urgently. [0003] Phosphorus accumulating bacteria (PAOs) are the main contributors to the biological phosphorus removal method. They can de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/36C12R1/40
CPCC12N1/36C12N1/205C12R2001/40
Inventor 田晴朱艳彬谢学辉杨波李方秦华星张成王康伟黄娜庄林杰孟丹杰王琦
Owner DONGHUA UNIV
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