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Method for testing disease resistant performance of wheat species through fungi bed germination and applications thereof

A disease resistance, wheat technology, applied in application, seed and rhizome treatment, agriculture, etc., can solve the problems of quality decline, wheat production reduction, etc., and achieve the effect of intuitive results, small investment, and easy operation

Active Publication Date: 2015-08-12
单桂玉 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tragic loss of wheat scab in 2010 has not yet been made up. In 2012, wheat scab occurred again in the winter wheat area in the southern part of the Yellow River and Huaihe River, resulting in a significant reduction in wheat production and quality.
[0008] In this field, we have not yet found a direct, simple and accurate detection and screening method that integrates the strengths of each family and overcomes the shortcomings of each family.

Method used

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  • Method for testing disease resistant performance of wheat species through fungi bed germination and applications thereof
  • Method for testing disease resistant performance of wheat species through fungi bed germination and applications thereof
  • Method for testing disease resistant performance of wheat species through fungi bed germination and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Testing wheat seeds for resistance to head blight:

[0084] (1) Wheat varieties to be tested: Four wheat varieties, Yannong 19 (ck), Jimai 22, Suyumai No. 1 and Xinmai No. 8 were selected respectively, and several samples were taken, each with 100 grains.

[0085] (2) Tools and materials: 12×12cm×5cm germination box, bud bed paper, tweezers, measuring cup, dropper, thermometer, head blight bacteria solution, water, etc.

[0086] (3) Test method:

[0087] 1. Obtain the source of germs:

[0088] At the mature stage of wheat, collect the ears of scab plants, and collect pink or purple scab kernels (such as figure 1 shown), bagged and stored.

[0089] 2. Prepare culture medium:

[0090] Mix wheat flour or corn flour with water and heat until the transparency is 65%, boil for 20 minutes every 3 hours to sterilize once, sterilize three times in total, then cool to room temperature, put it in a plastic or glass container, and seal the lid to get the product culture medium...

Embodiment 2

[0112] Testing wheat seeds for resistance to head blight at different temperatures:

[0113] (1) Wheat varieties to be tested: 3 batches of 100 seeds of different individual plants of Kaimai 18 were selected as samples.

[0114] (2) Tools and materials: 12cm×12cm×5cm germination box, bud bed paper, tweezers, measuring cup, dropper, thermometer, head blight liquid, water, etc.

[0115] (3) Test method:

[0116] 1. Obtain the source of germs:

[0117] At the mature stage of wheat, collect the ears of scab-affected plants, collect pink or purple-red scab-affected wheat kernels, and store them in bags.

[0118] 2. Prepare culture medium:

[0119] Mix wheat flour or corn flour with water and heat to 75% transparency, then boil for 20 minutes every 3 hours to sterilize once, sterilize three times in total, then cool to room temperature, put it into a plastic or glass container, and seal the lid. culture medium.

[0120] 3. Preparation of mother bacteria liquid or primary bacter...

Embodiment 3

[0142] Test the resistance of new strains to sheath blight with the basic dosage of sheath blight bacteria solution:

[0143] (1) Optional new strains of wheat to be tested: 1. Kaimai 18 anti-freeze line III6, 2. Radio No. 1 (Junmai 99-7-1 series), 3. Radio No. 2 (L01), 4. Kaimai Wheat 18I, 5, 99-7-2, CK New Wheat 21

[0144] (2) Test tools and materials: 12cm×12cm×5cm germination box, bud bed paper, tweezers, measuring cup, dropper, thermometer, sheath blight bacteria solution, water, etc.

[0145] (3) Test method

[0146] 1. Obtain the source of germs:

[0147] Collect the stalk base of the sheath blight plant, cut the part with obvious brown and black sheath blight spots into sections, and obtain the sheath blight straw section, which is stored in bags;

[0148] 2. Prepare culture medium:

[0149] Mix wheat flour or corn flour with water and heat to 70% transparency, then boil for 20 minutes every 3 hours to sterilize once, sterilize three times in total, then cool to r...

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Abstract

The invention relates to a novel method for testing the disease resistant performance of wheat species, namely a fungi bed germination testing method. The method comprises the following steps: (1) bacterium liquid processing: adding a bacterium liquid that can test the disease resistant performance of wheat into a germination box, and adding a little amount of water to prepare a fungi bed; then adding wheat grains to be tested into the germination box, and supplementing bacterium liquid until the embryo parts of wheat grains are soaked in the bacterium liquid; (2) water contrast treatment: placing the wheat grains on the paper bed of the germination box, and adding water until the water volume is equal to the total bacterium liquid volume; (3) calculating the bacterium hazard ratio; (4) judging the disease resistant performance: judging the disease resistant performance of wheat according to the national or provincial standards on the basis of the bacterium hazard ratio. The provided method has the advantages that the operation is simple and convenient, the results are direct and precise; the operation is easy, thus the error is reduced; the testing and screening periods are shortened; and the method is easy to promote.

Description

technical field [0001] The invention belongs to the technical field of wheat disease-resistant breeding, and in particular relates to a method for testing disease resistance of wheat varieties and screening disease-resistant materials and varieties (lines). Background technique [0002] Wheat head blight is also known as wheat ear dry, rotten wheat head, red wheat head. It mainly causes seedling rot, stem base rot, stalk rot and ear rot, which can be damaged from seedlings to heading. Among them, the most serious impact is ear rot. The tragic loss of wheat scab in 2010 has not yet been made up. In 2012, wheat scab occurred again in the winter wheat area in the south of the Yellow River and Huaihe River, resulting in a significant reduction in wheat production and quality. Susceptible varieties suffered particularly heavy losses, with yield reductions of more than 30%. Heavy losses of 70% to no harvest. The call for selection and breeding of disease-resistant varieties is...

Claims

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Application Information

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IPC IPC(8): A01C1/00
Inventor 单桂玉单永蕙
Owner 单桂玉
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