Breeding method for glutinous coix
A waxy coix and waxy technology, which is applied in the fields of botanical equipment and methods, seed and rhizome treatment, horticulture, etc., can solve the problems of increased difficulty in germplasm purification and rejuvenation, mixed varieties, slow variety breeding and renewal process, etc. Achieve the effect of purification and rejuvenation, single plant breeding, speeding up the breeding process, and simple and easy technical operation.
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Embodiment 1
[0018] Select coix germplasm from different sources, select 100-300 mature and plump seeds for each germplasm, peel off the shell and cut the endosperm at the end of the embryo to expose the cross-section of the endosperm, and use iodine reagent (the composition is potassium iodide (KI) 20 mg / mL, elemental iodine (I 2 ) 2 mg / mL) to dye the endosperm section for 5 minutes, wash with water for 3-5 minutes, wash off the floating color, observe the color of the section with the naked eye, and select the seeds with brownish red section for germination and seedling cultivation. The culture substrate is composed of humus and perlite, and the volume ratio is humus: perlite = 6:3, which is uniformly mixed and prepared; after the substrate is mixed, fill it into a plastic seedling tray, fill it to 2 / 3, and use Water the carbendazim solution with a mass fraction of 0.3% to sterilize the matrix, sow 1 seed in each hole and mark it, then sprinkle a thin layer of dry matrix on top of the se...
Embodiment 2
[0020] Select coix germplasm from different sources, select 100-300 mature and plump seeds for each germplasm, peel off the shell and cut the endosperm at the far embryo end, use iodine reagent (the composition is potassium iodide (KI) 20 mg / mL, iodine Simple substance (I 2 ) 2 mg / mL) to dye the endosperm section for 30 minutes, wash it with water for 3-5 minutes, wash off the floating color, observe the color of the section with the naked eye, and select the seeds with a brownish red section for germination and seedling cultivation. The culture substrate is composed of humus and perlite, and the volume ratio is humus: perlite = 5:1, which is uniformly mixed and prepared; after the substrate is mixed, fill it into a plastic seedling tray, fill it to 2 / 3, and use Water the carbendazim solution with a mass fraction of 3% to sterilize the matrix, sow 1 seed in each hole and mark it, then sprinkle a thin layer of dry matrix on top of the seeds (just cover the seeds), add a small a...
Embodiment 3
[0022] Select coix germplasm from different sources, select 100-300 mature and plump seeds for each germplasm, peel off the shell and cut the endosperm at the far embryo end, use iodine reagent (the composition is potassium iodide (KI) 20 mg / mL, iodine Simple substance (I 2 ) 2 mg / mL) to dye the section of endosperm for 10 minutes, wash it with water for 3-5 minutes, wash off the floating color, observe the color of the section with the naked eye, and select the seeds with a brownish-red section for germination and seedling cultivation. The culture substrate is composed of humus and perlite, and the volume ratio is humus: perlite = 6:3, which is uniformly mixed and prepared; after the substrate is mixed, fill it into a plastic seedling tray, fill it to 2 / 3, and use Water the carbendazim solution with a mass fraction of 0.3% to sterilize the matrix, sow 1 seed in each hole and mark it, then sprinkle a thin layer of dry matrix on top of the seeds (just cover the seeds), add a sm...
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