Primers, probes and methods for accurate detection of specific quantitative PCR of transgenic maize mon88017 strain
A technology of genetically modified corn and detection methods, which is applied in the directions of biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. To achieve the effect of protecting the right to know and the right to choose, high amplification efficiency and high accuracy
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[0035] The method for accurate detection of transgenic maize MON88017 strain-specific quantitative PCR mainly includes the following steps:
[0036] (1) Synthesize the following primers and fluorescent probes used in conjunction with the primers,
[0037] Upstream primer sequence: Event MON88017-Forward: 5'-CGCTAGCAGCTCTCCTCAA-3';
[0038] Downstream primer sequence: Event MON88017-Reverse: 5'-CCGGACATGAAGCCATTTACA-3';
[0039] Fluorescent Probe Sequence:
[0040] Event MON88017-Probe: 5'-FAM-CTTTTTTGCCGGAGTATGACGGTGACG-3'.
[0041] The synthesis concentrations of the primers and fluorescent probes in this example were both 10 μmol / l.
[0042] The nucleotide sequences of the above primers and fluorescent probes are designed for a specific site specific to the line of transgenic maize MON88017 and its products, that is, the target gene and the flanking site of the recipient maize genome; through this design, the transgenic maize can be accurately detected The transformation...
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