A quantitative method for the proportion of fetal free dna in the peripheral blood of pregnant women

A technology for the peripheral blood and fetus of pregnant women, applied in the field of DNA quantification, can solve the problems of low accuracy and easy deviation, and achieve the effect of improving accuracy

Active Publication Date: 2017-06-16
XIAMEN VANGENES BIOTECHNOLOGY CO LTD
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Problems solved by technology

[0011] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a method for quantifying the proportion of fetal free DNA in the peripheral blood of pregnant women, aiming to solve the problems of low accuracy and easy deviation in the existing methods for quantifying DNA in the peripheral blood of pregnant women

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  • A quantitative method for the proportion of fetal free dna in the peripheral blood of pregnant women

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specific Embodiment

[0091] The present invention introduces the DNA high-throughput sequencing method into the technology for measuring the proportion of fetal free DNA in pregnant women's free DNA, and the flow of the specific application process is further described in conjunction with specific examples.

[0092] 1. Materials

[0093] The samples provided were 4 cases of peripheral blood of pregnant women with unknown fetal cell-free DNA concentration, including 2 cases of male fetuses and 2 cases of female fetuses. DNA concentrations were compared.

[0094] High-throughput sequencing reagents are mainly composed of 3 kits, library construction kit (amplification library construction kit and ligation library construction kit); sequencing template preparation kit and sequencing kit.

[0095] 2. Method

[0096] The four samples were built and processed on the computer according to the above experimental steps to obtain sample data, and then data analysis was performed.

[0097] 1) Data process...

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Abstract

The invention discloses a quantitative method for the proportion of fetal free DNA in the peripheral blood of pregnant women. In the present invention, the designed primers are used to extract the DNA containing the designed SNP in the plasma, and after a series of treatments, the DNA is put on the machine, and the sequence product is compared to the human genome sequence to determine the base of each SNP site. The fetal cell-free DNA concentration was quantified by analyzing the ratio of each base in the SNP. The present invention can also correct the introduced error through a plurality of SNPs, and simultaneously calculate a plurality of SNPs with a statistical method, so as to improve the accuracy of the calculated fetal free DNA concentration.

Description

technical field [0001] The invention relates to the field of DNA quantification, in particular to a quantitative method for the proportion of fetal free DNA in the peripheral blood of pregnant women. Background technique [0002] Non-invasive prenatal genetic testing is to collect the peripheral blood (5ml) of pregnant women, extract the free DNA, use high-throughput sequencing technology, and combine biological information analysis to find that the fetus is suffering from chromosomal aneuploidy (21-trisomy, also known as Tang syndrome, 18-trisomy, 13-trisomy). Studies have found that from the 4th week of pregnancy, fetal cell-free DNA can be detected in the peripheral blood of pregnant women. As the gestational age increases, the content of fetal cell-free DNA also increases. After 12 weeks of pregnancy, by drawing the peripheral blood of the pregnant woman and extracting the fetal cell-free DNA, using the next-generation gene sequencing technology combined with bioinform...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 陈洪亮郑海灵祝兴强段利朋
Owner XIAMEN VANGENES BIOTECHNOLOGY CO LTD
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