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Preparation method and application of total saponins from flos hosta ventricosa

A technology of total saponins and purple hosta, which is applied in the field of medicine, can solve the problems of low purity and low yield of saponins, and achieve the effect of simple process, simple method and accelerated fracture

Active Publication Date: 2015-08-26
HARBIN UNIV OF COMMERCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the technical problems that the existing methods for extracting total saponins of Hosta japonica have less yield and the purity of saponins is not high

Method used

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  • Preparation method and application of total saponins from flos hosta ventricosa
  • Preparation method and application of total saponins from flos hosta ventricosa

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specific Embodiment approach 1

[0018] Specific embodiment one: the preparation method of the total saponins of Jaspa japonica in this embodiment is carried out according to the following steps:

[0019] Step 1. Take fresh Japa japonica, wash it and put it in a refiner, add ethanol with a volume fraction greater than or equal to 85% according to the mass ratio of Japa jasmine and ethanol at a ratio of 1:10 to 15, and heat up to 50°C for grinding 10min-15min, then filter or centrifuge, extract 3 times, combine the ethanol extracts, concentrate to obtain the extract. The extract is dispersed into a real solution with hot water, and then extracted with 1 to 3 times of water-saturated n-butanol for 5 to 8 times, combined with the n-butanol extract, and concentrated to obtain the n-butanol extract.

[0020] Step 2. Pretreat the AB8 macroporous resin, and then load it into a resin column. The n-butanol extract obtained in step 1 is dispersed into a true solution with hot water and loaded on the sample. First, it i...

specific Embodiment approach 2

[0034] Embodiment 2: This embodiment differs from Embodiment 1 in that: the AB8 macroporous resin described in step 2 is replaced with HPD826 macroporous resin. Other steps and parameters are the same as in the first embodiment.

specific Embodiment approach 3

[0035] Embodiment 3: This embodiment differs from Embodiment 1 in that: the AB8 macroporous resin described in step 2 is replaced with D101 macroporous resin. Other steps and parameters are the same as in the first embodiment.

[0036] Adopt following experiment verification invention effect

[0037] Weigh each 15g of pretreated macroporous adsorption resins of AB-8, D101, HPD100, H103, HPD826, and NKA-96 types, put them in a 100mL beaker, and add 40mL of total saponins solution (total saponins content of 6.26mg / mL), put it in a shaker and vibrate for 8 hours, then filter with suction, collect the filtrate, and measure the quality of saponin in the filtrate with ultraviolet light, which is M residue. Then put the resin into a chromatographic column (with an inner diameter of 1.5cm and a height of 20cm), with a diameter-to-height ratio of 1:9, and remove impurities with 3BV distilled water at a flow rate of 2-3BV / h, collect the water, and measure the saponin quality, which is ...

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Abstract

The invention discloses a preparation method and application of total saponins from flos hosta ventricosa, belongs to the technical field of medicine, and aims to solve the technical problems that the existing method for extracting total saponins from flos hosta ventricosa is low in yield and purity of total saponins. The preparation method comprises the following steps: Step one, taking fresh flos hosta ventricosa to be cleaned, placing the cleaned flos hosta ventricosa into a refining machine, adding ethanol to obtain a mixture I, heating the mixture I to 50 DEG C, grinding the mixture I, filtering or centrifuging the mixture I to volatilize ethanol and obtain a solution I, and concentrating the solution I to obtain an extract; Step two, pre-treating an AB8 macroporous resin, filling the pretreated resin into a resin column, loading a sample, eluting the sample with distilled water until Molish reaction is negative, eluting the sample with 20% ethanol to remove impurities, eluting the sample with 50%-70% ethanol, recovering a solvent after collecting ethanol eluent, and evaporating the ethanol eluent to dryness. According to the invention, the total saponins from flos hosta ventricosa are applied to the preparation of an antitumor drug and are applied to health care food and cosmetics as a natural antioxidant; the purity of the total saponins from flos hosta ventricosa is higher than 60%.

Description

technical field [0001] The invention belongs to the technical field of medicine; in particular, it relates to a new method for preparing total saponins of hosta japonica. Background technique [0002] Hosta ventricosa Stearn (Hosta ventricosa Stearn) is a perennial herb of the Liliaceae Hosta genus. "National Compilation of Traditional Chinese Medicine" records that purple jade hairpin has the effects of dispelling blood stasis, relieving pain, and detoxifying, and can treat bruises, stomach pain, sore throat and other diseases. The main chemical components are steroidal saponins, flavonoids (kaempferol glycosides), phenylpropanoids (7-hydroxycoumarin) and so on. Our previous research found that the purple calyx hosta flower has anti-tumor effect. After tracking and screening by MTT method, it was found that the total steroidal saponins are the anti-tumor active parts of Hosta japonica, and have a significant anti-proliferation effect; at the same time, it was found that i...

Claims

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Application Information

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IPC IPC(8): A61K36/896A61K8/97A23L1/30A61P35/00A61P39/06A61Q19/08
Inventor 邹翔曲中原
Owner HARBIN UNIV OF COMMERCE
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