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Method For Fluorescence Microscopy Of A Sample

A technique of fluorescence microscopy and microscopy, which is used in the field of observing samples by fluorescence microscopy, and can solve the problems of accommodation, poor signal-to-noise ratio, etc.

Inactive Publication Date: 2015-09-23
CARL ZEISS MICROSCOPY GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This can either be compensated by extending the camera capture time, or a worse signal-to-noise ratio has to be accommodated

Method used

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  • Method For Fluorescence Microscopy Of A Sample
  • Method For Fluorescence Microscopy Of A Sample
  • Method For Fluorescence Microscopy Of A Sample

Examples

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Embodiment Construction

[0029] figure 1 A microscope 1 for the fluorescence microscopy observation of a sample P lying in the sample plane 2 is shown schematically. The sample is imaged along the optical axis OA into the camera chip plane 3 in which the camera chip of the microscope camera is located. The microscope 1 is designed such that the microscope camera can be exchanged, that is to say that different camera chips can be arranged in the camera chip plane 3 , which in particular have different extents and / or azimuth ratios.

[0030] The imaging of the sample P along the optical axis OA in the sample plane 2 takes place via an imaging beam path 4 which has, in particular, an objective lens 5 and a tube lens 6 . The illumination beam path 7 for incident light illumination of the sample P is reflected by means of a beam splitter 8 into the imaging beam path 4 . Light is directed from the light source 9 to the sample P through the imaging path 4 . The imaging beam path 4 and the illumination beam ...

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Abstract

A method for fluorescence microscopy of a sample. The method includes illuminating the sample with excitation radiation of a microscope through an illumination beam path and imaging along an optical axis to an image field on a camera chip, the illumination beam path having an adjustable diaphragm for selective illumination of a target area; selecting an area on the sample as a target area; and adjusting an adjustable diaphragm for selective illumination of the target area such that parts of the sample lying outside of the selected area are not illuminated with excitation radiation.

Description

technical field [0001] The invention relates to a method for observing a sample by fluorescence microscopy, in which a microscope is used which illuminates the sample passing through the illumination path with an excitation beam and images it along the optical axis onto a camera chip , wherein the illumination path has an adjustable diaphragm for selectively illuminating the target area, an area on the sample is selected as the target area, and the adjustable diaphragm for selectively illuminating the target area is The adjustment is such that parts of the sample lying outside the selected area are not illuminated by the excitation beam. Background technique [0002] In the field of biological sciences, microscopes play an important role. Biological samples can be present on various sample carriers, for example between slides and coverslips, in Petri dishes or microtiter plates. The biological sample can be alive or fixed as a specimen, unstained or stained. Usually, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/06G02B21/36
CPCG02B21/0076G02B21/06G02B21/082G02B21/16G02B21/365G01N21/6458
Inventor 彼得·舒恩托尔斯滕·库斯塞巴斯蒂安·罗德
Owner CARL ZEISS MICROSCOPY GMBH
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