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Serratia marcescens strain and application thereof
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A Serratia marcescens, plant technology, applied in the field of microorganisms, to achieve good adsorption effect and good control effect
Active Publication Date: 2015-09-30
天津沃太生物科技有限公司
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Problems solved by technology
[0004] In addition, at present, there are different degrees of lead pollution in the soil of various regions in my country.
Method used
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Embodiment 1
[0030] The isolation and identification of embodiment one Serratia marcescens bacterial strain Sa
[0031] 1. Isolation of Serratia marcescens strain Sa
[0032] Isolate and obtain from cucumber rhizosphere soil in Xiqing District, Tianjin City by a method comprising the following steps:
[0034] (2) The dilution in the drawing step (1) is 1:10 2 , 1:10 3 , 1:10 4 , 1:10 5 , 1:10 6 100 μL of the soil dilution solution was applied to LB solid medium containing 400, 600, 800, 1000, 1200 and 1400 mg / l lead nitrate, respectively, and cultured at 28°C for 1 day;
[0035] (3) Pick a single colony grown after 1 day of cultivation, and then culture it purely on an LB medium plate to obtain strain Sa.
[0036] 2. Morpho...
Embodiment 2
[0055] Determination of the control ability of embodiment two Serratia marcescens strain Sa to cucumber wilt
[0056] 1. Preparation of spore suspension of Fusarium wilt of cucumber: Take the Fusarium wilt of cucumber cultured on PDA medium by a conventional culture method, inoculate it in PD medium, and cultivate it at 25°C and 100r / min for 7 days. Then, the culture solution was filtered and placed in a centrifuge for 10 minutes at 4000r / min, the supernatant was discarded, diluted with sterile water, the amount of spores was measured by a hemocytometer, examined under a microscope, and diluted to 5.8×10 6 CFU / ml;
[0057] 2. Preparation of strain Sa fermentation broth: inoculate a single colony of strain Sa in 5 mL of LB liquid medium after streak culture in LB solid medium for 24 hours at 28°C, and then inoculate at 28°C and 200r / min Shake culture under the conditions for 12 hours, which is the strain Sa seed liquid, then take 100 μL of the strain Sa seed liquid into 100 mL...
Embodiment 3
[0070] Determination of the growth-promoting effect and colonization ability of embodiment three Serratia marcescens strain Sa to cucumber seedlings
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Abstract
The invention relates to a Serratia marcescens strain of which the collection number in China General Microbiological Culture Collection Center is CGMCC No.10711. The strain Sa is separated from soil. The strain Sa is identified to be Serratia marcescens through the observation on morphological features of the strain Sa, analysis on physiological biochemical characteristics and homology analysis on the 16Sr DNA (deoxyribonucleic acid) sequence. The Serratia marcescens strain provided by the invention has favorable control effect on blight of plants. The Serratia marcescens strain can be colonized in plant seedlings, and has obvious growth promoting actions on the seedlings. Besides, the determination of concentration changes of Pb<2+> in the culture medium by an atomic absorptiometry indicates that the Serratia marcescens strain can adsorb Pb<2+> in the culture medium and has favorable adsorbing effect.
Description
technical field [0001] The invention relates to the field of microbes, and more specifically to a Serratia marcescens capable of promoting growth and bioremediating soil and its application. Background technique [0002] PlantGrowth PromotingRhizobacteria (PGPR) refers to a class of bacteria living freely in the soil or attached to plant roots that can promote plant growth and its absorption and utilization of mineral nutrients, and can inhibit the beneficial effects of harmful organisms. fungus. Applying PGPR microorganisms when a disease occurs can disturb the plant-pathogen interaction and create a new balance that benefits the plant. [0003] The mechanism of PGPR microbial disease prevention and growth promotion is the result of the comprehensive action of various methods, including PGPR microbial transformation of nutrients that plants cannot directly use in the soil, production of phytohormones that regulate plant growth, production of volatile gases, and productio...
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