Pretreatment method of mesenchymal stem cells and preparation obtained from mesenchymal stem cells

Abstract

The invention discloses a pretreatment method of mesenchymal stem cells and a preparation obtained from the mesenchymal stem cells. The pretreatment method comprises the steps that the mesenchymal stem cells are cultured in a culture medium in a low oxygen environment, and the oxygen concentration of the adopted low oxygen environment is gradually reduced from 21 percent along with culture time. The stem cell preparation cultured through the pretreatment method can effectively adapt to the low-oxygen state of adipose tissue of a human body, and the prevention and treatment effect on diabetes can be improved.

Description

technical field [0001] The invention relates to a pretreatment method for culturing mesenchymal stem cells in a hypoxic environment, belonging to the field of biotechnology. Background technique [0002] Mesenchymal stem cells (Mesenchymal Stem Cells, MSCs) are stem cells derived from the mesoderm and ectoderm in the early stages of development. Mesenchymal stem cells have the functions of supporting hematopoiesis and regulating immunity, and have effects that traditional chemical drugs cannot achieve in hematopoietic reconstruction, tissue repair, and immunotherapy, so they have received extensive attention in medical and clinical research. [0003] At present, the way of culturing mesenchymal stem cells is to separate them from the source tissue, culture them in an atmospheric environment, and pass through 4 to 6 generations to expand them to a certain number before they can be used in diseases. intervene. Studies have found that the oxygen concentration in the healthy h...

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Problems solved by technology

However, long-term studies have also found that there are several defects in this technical solution, which are highlighted in that the adjustment of oxygen concentration is too aggressive, which makes the survival rate of mesenchymal stem cells during the culture process too low; more importantly, the pretreatment method adjusts the The biological characteristics of mesenchymal stem cells make it impossible to exert the disease intervention and treatment characteristics of normal mesenchymal stem cells in the human environment under normal diseases

Due to the existence of the above defects, the program has been in the research stage for many years and cannot meet the needs of clinical intervention and treatment.

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