Pretreatment method of mesenchymal stem cells and preparation obtained from mesenchymal stem cells

A mesenchymal stem cell and pretreatment technology, applied in the field of biotechnology, can solve the problems of inability to meet the needs of clinical intervention treatment, inability to exert the characteristics of mesenchymal stem cell disease intervention and treatment, and low survival rate of mesenchymal stem cells

Inactive Publication Date: 2015-09-30
龚伟
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Problems solved by technology

However, long-term studies have also found that there are several defects in this technical solution, which are highlighted in that the adjustment of oxygen concentration is too aggressive, which makes the survival rate of mesenchymal stem cells during the culture process too low; more importantly, the pretreatment method adjusts the The biological characteristics of mesenchymal stem cells make it impossible to exert the disease intervention and treatment characteristics of normal mesenchymal stem cells in the human environment under normal diseases
Due to the existence of the above defects, the program has been in the research stage for many years and cannot meet the needs of clinical intervention and treatment.

Method used

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  • Pretreatment method of mesenchymal stem cells and preparation obtained from mesenchymal stem cells

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Embodiment 1

[0019] Embodiment 1: the pretreatment method disclosed by the present invention is preferably carried out by the following process:

[0020] Mesenchymal stem cells were cultured with basal medium and supplements. In this example, 500 ml of DMEM was chosen as the base medium, and the supplements included 25 ml of fetal (small) bovine serum, 5 ml of growth factors, and 3 ml of amino acids. This is not limiting, and those skilled in the art can select other suitable types and components as needed.

[0021] At the 3rd to 4th passage, collect the mesenchymal stem cells that adhere to the wall, transfer them to a new culture bottle, transfer them to a hypoxic incubator, and cultivate them for 48 hours: the mesenchymal stem cells transfer into the hypoxic incubator. Reduce the oxygen concentration by 1% in the oxygen incubator, and then reduce the oxygen concentration in the hypoxic incubator by 1% every 4 hours until the final oxygen concentration drops to 9% (the carbon dioxide co...

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Abstract

The invention discloses a pretreatment method of mesenchymal stem cells and a preparation obtained from the mesenchymal stem cells. The pretreatment method comprises the steps that the mesenchymal stem cells are cultured in a culture medium in a low oxygen environment, and the oxygen concentration of the adopted low oxygen environment is gradually reduced from 21 percent along with culture time. The stem cell preparation cultured through the pretreatment method can effectively adapt to the low-oxygen state of adipose tissue of a human body, and the prevention and treatment effect on diabetes can be improved.

Description

technical field [0001] The invention relates to a pretreatment method for culturing mesenchymal stem cells in a hypoxic environment, belonging to the field of biotechnology. Background technique [0002] Mesenchymal stem cells (Mesenchymal Stem Cells, MSCs) are stem cells derived from the mesoderm and ectoderm in the early stages of development. Mesenchymal stem cells have the functions of supporting hematopoiesis and regulating immunity, and have effects that traditional chemical drugs cannot achieve in hematopoietic reconstruction, tissue repair, and immunotherapy, so they have received extensive attention in medical and clinical research. [0003] At present, the way of culturing mesenchymal stem cells is to separate them from the source tissue, culture them in an atmospheric environment, and pass through 4 to 6 generations to expand them to a certain number before they can be used in diseases. intervene. Studies have found that the oxygen concentration in the healthy h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K35/28A61P3/10
Inventor 龚伟
Owner 龚伟
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