Tree peony bark extract and application thereof to preparation of medicines for treating pulmonary fibrosis

A technology of Moutan Cortex extract and pulmonary fibrosis, applied in the field of medicine, can solve problems such as pulmonary fibrosis in the effective part of Moutan Cortex

Active Publication Date: 2015-10-21
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, up to now, there is no relevant research report on the prevention and treatment

Method used

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  • Tree peony bark extract and application thereof to preparation of medicines for treating pulmonary fibrosis
  • Tree peony bark extract and application thereof to preparation of medicines for treating pulmonary fibrosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Cortex Moutan extract of the present invention is extracted by the following method:

[0045] a. Take Moutan Cortex as raw material, add pectinase aqueous solution accounting for 10 times the weight of Moutan Cortex raw material, then place it in a flash extractor, and extract at room temperature for 3 minutes; add hydrochloric acid to the extracted medicinal material and the solution to adjust the obtained extraction The pH value of the solution was adjusted to 4.5, and after adjustment, the water bath was heated to 45°C for enzymolysis, and the enzymolysis was carried out for 2 hours to obtain the enzymolysis solution;

[0046] B, adopt the steam distillation method to collect the distillate accounting for 1 / 3 of the total weight of the enzymolysis solution, the gained distillate is refrigerated and placed for 24 hours at 4°C, and filtered under this condition, the gained crystal is paeonol (paeonol) Phenol purity ≥ 90%);

[0047] c. The remaining extract and dregs a...

Embodiment 2

[0052] Cortex Moutan extract of the present invention is extracted by the following method:

[0053] a. Take Moutan Cortex as raw material, add pectinase aqueous solution accounting for 15 times the weight of Moutan Cortex raw material, then place it in a flash extractor, and extract at room temperature for 2 minutes; add sulfuric acid to the extracted medicinal material and solution to adjust the obtained extraction The pH value of the solution was adjusted to 4.0, and after adjustment, the water bath was heated to 40°C for enzymolysis, and the enzymolysis was carried out for 1.5 hours to obtain the enzymolysis solution;

[0054] B, adopt the steam distillation method to collect the distillate accounting for 1 / 2 of the total weight of the enzymolysis solution, the gained distillate is refrigerated and placed for 24 hours at 4°C, and filtered under this condition, the gained crystal is paeonol (paeonol) Phenol purity ≥ 90%);

[0055] c. The remaining extract and dregs after d...

Embodiment 3

[0060] Cortex Moutan extract of the present invention is extracted by the following method:

[0061] a. Take Moutan Cortex as raw material, add pectinase aqueous solution accounting for 6 times the weight of Moutan Cortex raw material, then place it in a flash extractor, and extract at room temperature for 5 minutes; add sulfuric acid to the extracted medicinal material and solution to adjust the obtained extraction The pH value of the solution is adjusted to 3.8, and after adjustment, the water bath is heated to 60°C for enzymolysis, and the enzymolysis is carried out for 1 hour to obtain the enzymolysis solution;

[0062] B, adopt the steam distillation method to collect the distillate accounting for 1 / 4 of the total weight of the enzymolysis solution, and the gained distillate is refrigerated and placed at 4°C for 24 hours, and filtered under this condition, the gained crystal is paeonol (paeonol) Phenol purity ≥ 90%);

[0063] c. The remaining extract and dregs after dist...

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Abstract

The invention discloses a tree peony bark extract and application thereof to preparation of medicines for treating pulmonary fibrosis. At first, tree peony bark is extracted, and after that, pH value adjustment and heating for enzymolysis are carried out; after enzymolysis, the distillate is collected by the steam distillation method, left to stand and filtered to obtain crystal paeonol; the residual extracting solution and the leaves are filtered, and the herb residues are left for later use; the filtrate is subjected to concentration under reduced pressure and deproteinization, then ethanol is added, the mixture is left to stand, the ethanol solution is separated and collected for later use, and the sediment is washed to obtain cortex moutan polysaccharide; the stand-by ethanol solution is added into the stand-by herb residues for soaking and filtering, and the filtrate is subjected to concentration under reduced pressure and purification to obtain total glucosides of cortex moutan; the paeonol, the polysaccharide and the total glucosides are mixed to obtain the tree peony bark extract. Experimental results show that the tree peony bark extract can effectively inhibit epithelial-mesenchymal transition and lung fibroblast activation of alveolar epithelial cells so as to prevent pulmonary fibrosis from formation and development, and can be applied to preparation of medicines for treating pulmonary fibrosis.

Description

1. Technical field: [0001] The invention relates to the technical field of medicine, in particular to an extract of Cortex Moutan and its use in preparing medicines for preventing and treating pulmonary fibrosis. 2. Background technology: [0002] Pulmonary fibrosis is a malignant disease that seriously threatens human health and life. The average survival time of pulmonary fibrosis is 3 years after diagnosis, or 5 years after the onset of clinical symptoms. In the terminal stage of pulmonary fibrosis, lung transplantation is the only effective treatment. However, the lack of donors and high medical expenses limit the application of lung transplantation. Improving the efficacy of drugs has always been the goal pursued by medical workers. With the deepening of research on the pathogenesis of pulmonary fibrosis, it has been confirmed that the excessive accumulation of collagen, elastic fibers and protein sugars in the extracellular matrix (ECM) is its characteristic histopath...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61P11/00
Inventor 李建生余海滨沈俊岭任周新李俊凯
Owner HENAN UNIV OF CHINESE MEDICINE
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