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A kind of production method and application of 10,11-dehydrocurvularcin

A technology of curvularycin and curvularia, applied in the fields of application, botany equipment and methods, medical preparations containing active ingredients, etc., can solve the problems of unseen microbial fermentation and refining process, and achieve strong adaptability Effect

Active Publication Date: 2019-02-19
CHINA THREE GORGES UNIV
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Problems solved by technology

More reports focus on the isolation and identification of compounds, and there is no microbial fermentation and refining process for such compounds

Method used

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  • A kind of production method and application of 10,11-dehydrocurvularcin
  • A kind of production method and application of 10,11-dehydrocurvularcin
  • A kind of production method and application of 10,11-dehydrocurvularcin

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Embodiment Construction

[0022] The following tests and examples are used to further illustrate but not limit the present invention.

[0023] A method for producing 10,11-dehydrocurvularcin by microbial fermentation, the process of which is as follows:

[0024] (1) Preparation of shake flask seed liquid: Inoculate the slant strains into a PDA solid culture dish for activation, and cultivate them at 30°C for 72 hours. The growth of the bacteria was observed by the naked eye, and there was no contamination by bacteria ( figure 1 ). Under the aseptic operating environment, use an inoculation needle to draw a 0.5cm×0.5cm bacterial block and inoculate it in a seed shaker flask for cultivation. The seed culture shaker flask was placed in a constant temperature shaker with a rotational speed of 120 r / min, and the culture temperature was maintained at 28° C., and cultured for 7 days to obtain liquid strains.

[0025] (2) Fermentation culture: The culture liquid was prepared according to the formula of the s...

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Abstract

The invention belongs to the field of microbial fermentation and particularly relates to a method for producing 10, 11-dehydrogenated curvularin through a microbiological fermentation method. The method is characterized in that the liquid fermentation technology is used for obtaining a fermentation substrate, a filtering method is utilized for removing mycelia, then extraction is carried out through ethyl acetate, decompressing condensing is carried out so that concrete is obtained, the ethyl acetate re-crystallization technology is utilized for obtaining a refined 10, 11-dehydrogenated curvularin product, and the purity is over 99%. Strains are high in adaptability to fermentation raw materials, and the separation yield of the 10, 11-dehydrogenated curvularin product reaches 28.8%. The 10, 11-dehydrogenated curvularin product has multiple pharmacological activities such as weed prevention and removal and tumor resistance, and is widely applied to the fields of medicine, biological pesticide and the like.

Description

technical field [0001] The invention relates to a method for producing and refining 10,11-dehydrocurvularycin by microbial fermentation, which belongs to the field of microbial fermentation. Background technique [0002] 10,11-dehydrocurvularin (10,11-dehydrocurvularin) is an excellent natural herbicide, in addition, it also has a variety of biological activities, such as toxicity to human lung fibroblasts, TGF-β inhibitory activity, Antioxidant activity, and antibacterial activity. Turner W.B. (Fungal Metabolites, 1971, Academic Press, London) isolated 10,11-dehydrocurvularin from the metabolites of the fungus Curvularia sp. for the first time, and no yield was reported. More reports focus on the isolation and identification of compounds, but there is no microbial fermentation and refining process for such compounds. Contents of the invention [0003] The purpose of the present invention is to provide a method for producing high-purity 10,11-dehydrocurvularycin by micro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/08A01N43/22A01P13/00A61K31/365A61P35/00
Inventor 邓张双陈良立郭志勇龚大春汪鋆植邹坤
Owner CHINA THREE GORGES UNIV
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