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Polysaccharides in tree lucidum and its extraction method and application

An extraction method and polysaccharide technology, which is applied in chemical instruments and methods, oligosaccharides, sugar derivatives, etc., can solve the problems of insufficient research on polysaccharides from Reedia chinensis, and achieve remarkable results, simple preparation process, and controllable quality

Active Publication Date: 2017-06-06
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, domestic and foreign research on Bozhizhi mainly focuses on the pharmacological activity of Bozhizhi glycopeptides, and the research on Bozhizhi polysaccharides is not deep enough. At the same time, there are few reports on the research on the polysaccharides of Bozhizhi fermented products.

Method used

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  • Polysaccharides in tree lucidum and its extraction method and application
  • Polysaccharides in tree lucidum and its extraction method and application
  • Polysaccharides in tree lucidum and its extraction method and application

Examples

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Effect test

Embodiment 1

[0073] Example 1 The extraction method of polysaccharides in Boga tree lucidum

[0074] 1) Degreasing: Degrease 3.8kg of Bolus baccatum fungus powder with 5 times the volume of petroleum ether, and then dry it;

[0075] 2) Water extraction: extract the degreased A. bolus mushroom powder with 10 times the volume of hot water (60-100°C) for 2 hours, collect the extract, and dry the residue.

[0076] 3) Fractional alcohol precipitation: After the extract was concentrated in vacuo at 60°C, ethanol was added to make the ethanol volume concentration 40%, and after standing at room temperature for 24 hours, centrifuged, collected the precipitate, weighed 981.16g, and it was the crude polysaccharide Pa; collected the supernatant After concentrating in vacuo at 60°C, add ethanol to make the ethanol volume concentration 70%, let it stand at room temperature for 24 hours, centrifuge, collect the precipitate, weigh 158.35g, it is crude polysaccharide Pb, repeat the same operation as above...

Embodiment 2

[0079] Example 2 Extraction method of polysaccharide GCP-5

[0080] 1) Degreasing: Degrease 3.8kg of Bolus baccatum fungus powder with 5 times the volume of petroleum ether, and then dry it;

[0081] 2) Water extraction: extract the degreased A. bolus mushroom powder with 10 times the volume of hot water (60-100°C) for 2 hours, collect the extract, and dry the residue.

[0082] 3) Alkaline extraction: Submerge the residue after water extraction in 15 times the volume of 0.3M NaOH solution, leave it at room temperature for 2 hours, neutralize the supernatant with 0.5M HCl to make it PH=6~8, take the supernatant Carry out centrifugation (5000r / min, 10min), take the supernatant, concentrate in vacuo at 60°C, then add ethanol to make the volume concentration of ethanol 70%, let it stand for 24h, then centrifuge, collect the precipitate, weigh 93.87g, this is alkaline extraction Crude polysaccharide PB.

[0083] 4) Purification: The crude polysaccharide PB was deproteinized by th...

Embodiment 3

[0088] The method for extracting the polysaccharide GCP-4 of embodiment 3

[0089] The extraction method of GCP-4 is the same as the extraction method of GCP-5 in Example 2, except that in step 6) when molecular sieve gel chromatography is carried out, the peak one polysaccharide sample in step 5) is used, and the Sephadex G75 gel During the column purification process, elution with water resulted in two peaks, and the eluate from peak two was collected, concentrated and freeze-dried to obtain GCP-4. Others are all the same as embodiment 2.

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Abstract

The present invention discloses polysaccharides in ganoderma capense, an extraction method and applications thereof. According to the extraction method, ganoderma capense hypha powder is adopted as a raw material, and combination of waster extraction alcohol precipitation extraction and alkali extraction, Sevag method deproteining, DEAE cellulose column chromatography, Sephadex column chromatography and the like are performed to obtain the polysaccharide pure product, wherein the structure analysis results show that the ganoderma capense polysaccharides obtained through the method comprise heteropolysaccharide and homopolysaccharide and the molecular weight range is 1000-200000 Da; and the extraction method is performed under relatively mild conditions, the polysaccharide components are well retained, and the obtained polysaccharides have characteristics of clear structure and controllable quality. According to the present invention, the ganoderma capense polysaccharides provide significant effects in the field of immunity enhancing and anti-tumor; and the anti-oxidation experiment results show that obtained polysaccharide pure product has the DPPH. and OH. Free radical scavenging activity so as to provide basis for application in the fields of food, cosmetics , health products, medicine and the like.

Description

technical field [0001] The invention belongs to the technical field of medicine and health food, and specifically relates to plant crude polysaccharides, pure polysaccharides and their preparation methods and uses. Background technique [0002] Boshuzhi ( Ganoderma Capense (Lloyd) Teng) is the mycelium of the subgenus Ganoderma lucidum, also known as Ganoderma lucidum and Bozhi, and it is a fungus of the genus Ganoderma in the Polyporaceae family. Slightly sweet and cold in nature, it can clear away heat and reduce inflammation, and treat mainly hepatitis and tumors. It is widely distributed in China, Japan, South Korea and other Asian countries. [0003] According to reports, the chemical components of Boshuzhi include purine, pyrimidine, D-mannitol, lectin, alkaloid, ergosterol, ergosterol palmitate, ergoster-7,22-dien-(3)one, ergosterol- 7. Steroidal compounds such as 22-diene-3β-ol, 5α-stigmasterane-(3,6)dione, β-sitosterol, bean saponin-B, and arachidic acid, stearic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02C07H1/08C07H3/06A61P37/04A61P35/00A61P39/06
Inventor 严春艳黄雅婷黎乃生
Owner GUANGDONG PHARMA UNIV
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