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Method for increasing cross compatibility and seed setting rate of tobacco and N.alata

A technology for cultivating tobacco and tobacco, applied in the field of agricultural biology, can solve the problems of inability to enter the ovary and inability to obtain seeds, and achieve the effects of easy promotion, low cost and simple operation

Inactive Publication Date: 2015-10-28
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Our research shows that: ♀Nicotiana tabacum(gla.)S In the process of alata hybridization, when the female parent flower is in the beginning stage of pollination, the pollen tube growth of N.alata is blocked and stays at the base of the female parent style 0.81±0.10cm away from the ovary, unable to enter the ovary, let alone obtain seeds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The concrete steps of this method are as follows:

[0013] 1. Delayed pollination overcomes hybrid incompatibility

[0014] a. Select 150 flowers of cultivated tobacco, that is, the female parent Nta (gla.) SK326) at the beginning of flowering, and put them on the list. The timing from the time of listing is 0h after the beginning, and 50 of them will be counted at 72h, 96h and 120h after the listing. Pollination of the female stigma with the mature pollen of Nicotiana tabacum, the male parent N.alata, and isolation with nylon mesh bags after pollination;

[0015] b. After 48 hours of pollination, remove the nylon mesh bag, peel off the pistil, store it in FAA fixative solution, place it at 4°C for more than 2 hours; soften it with NaoH for 120 minutes, stain it with 0.1% water-soluble aniline blue for more than 2 hours, and press it under fluorescence The growth status of the pollen tubes was observed under a microscope, and the pollen tube growth hindering parts in 2...

Embodiment 2

[0022] The method of delaying pollination 120h after the beginning of the seeding rate of hybridization is the same as that in Example 1, the difference lies in the ratio of the stigma grind and the pollen of the male parent.

[0023] a. Select cultivated tobacco, that is, the female parent Nta (gla.) SK326) at the beginning stage of flowering, strip off the pistil and cut the stigma, dry the stigma in the room for 2 days, crush the stigma into powder, and the flower tobacco, the male parent N .alata mature pollen and stigma powder are mixed in a weight ratio of 1:20 and 1:50;

[0024] b. use the pollen mixed in a weight ratio of 1:20, 1:50 to pollinate the flowers of the cultivated tobacco, that is, the female parent Nta (gla.) SK326) 120h after the beginning period, and the follow-up treatment is the same as that of delayed pollination in Example 1 to overcome hybridization Steps b and c in the incompatibility step. The results showed that the mixed pollen of 1:5 and 1:10 w...

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Abstract

The invention provides a method for increasing the cross compatibility and the seed setting rate of tobacco and N.alata, and belongs to the technical field of agricultural biology. The method includes the steps of starting hanging female parent flowers, wherein the hanging time is the flowering start time, and the 72nd hour, the 96th hour and the 120th hour after hanging are the aging stages after the flowering start time; pollinating female parent stigmas with male parent mature pollen during the three time periods, conducting sampling 48 hours after pollination, counting the number of pollen tubes and the seed setting rate through callose fluorescence, and comparing influences of pollination on the cross compatibility; selecting flowers, at the flowering start time, of female parents, stripping pistils, clipping stigmas which are smashed into powder after being naturally aired indoors, and mixing the male parent mature pollen with sigma powder according to the weight ratio between 1 to 5 and 1 to 50; pollinating the flowers with mixed pollen 120 hours after the flowering start time of the female parents, conducting sampling 48 hours after pollination, counting the number of pollen tubes and the seed setting rate through callose fluorescence, and comparing influences of pollination on the cross compatibility; cultivating tobacco as the female parents, and planting N.alata as the male parents. The method has the advantages that operation is simple, cost is low, popularization is easy, and reference is provided for obtaining of plant inter-species coeno-species.

Description

Technical field: [0001] The invention relates to a method for improving the hybrid affinity and seed setting rate of cultivated tobacco and Nicotiana tabacum, belonging to the field of agricultural biotechnology. Background technique: [0002] Distant hybridization between plant species can break the boundaries between species to a certain extent, and recombine the beneficial traits accumulated by two species through long-term evolution to form new yield, quality traits and resistance to diseases, insects, cold, drought, Waterlogging and other stress resistance (tolerance), etc. Distant hybridization has played an important role in the production and breeding of plants such as wheat, cotton, rice and tomato. However, in many crop cross-breeding combinations, interspecific genetic background differences and reproductive isolation often lead to hybrid incompatibility. This phenomenon is also commonly found in Nicotiana plants, including hybrid incompatibility before fertiliz...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 陈穗云廖菊够李永平马文广
Owner YUNNAN UNIV
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