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A method for the mineralization of heavy metal ions using microbial degradation of glyphosate

A heavy metal ion and microbial degradation technology, applied in the direction of chemical instruments and methods, applications, fertilizer mixtures, etc., can solve problems such as high energy consumption, secondary pollution, complex operation, etc., achieve stable mineral properties, strong durability, and slow down the greenhouse effect of effect

Active Publication Date: 2017-03-15
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Traditional restoration methods mainly use physical methods and chemical methods, but they are not suitable for large-scale pollution restoration due to high energy consumption, large investment, complicated operation, and easy to produce secondary pollution.

Method used

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  • A method for the mineralization of heavy metal ions using microbial degradation of glyphosate
  • A method for the mineralization of heavy metal ions using microbial degradation of glyphosate
  • A method for the mineralization of heavy metal ions using microbial degradation of glyphosate

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example

[0036] (1) Weigh 6g of peptone, 4g of beef extract, 5g of NaCl, 18g of agar, MgSO 4 Dissolve 0.5g in 1000ml of deionized water, and control the pH to 7, sterilize at 125°C for 30 minutes, take it out and wait for cooling; inoculate Bacillus subtilis into the cooling medium solution, and culture it with shaking at 37°C, the shaking frequency is 170r / min, culture time 24h; centrifuge the cultivated bacterial liquid at high speed for 10min, the speed of the centrifuge is 8000rpm, the temperature is 4°C, remove the nutrients in the upper medium, add 100mL of deionized water to make concentrated bacterial liquid.

[0037] (2) Weigh 18g of agar and dissolve it in 1000ml of wort at 5°C, sterilize at 125°C for 30 minutes, take it out and wait for cooling; inoculate the yeast into the cooling medium solution, and culture at 30°C with shaking at a frequency of 170r / min, culture time 24h; Centrifuge the cultured bacterial solution at high speed for 10min, the speed of the centrifuge is...

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Abstract

The invention discloses a microbial remediation method capable of mineralizing heavy metal ions based on glyphosate degradation. The microbial remediation method comprises the following steps of: separately inoculating bacillus subtilis, saccharomycetes and bacillus mucilaginosus into corresponding culture mediums for cultivation so as to prepare concentrated bacterial liquids with the cell concentration of 10<6>-10<7> / mL; uniformly mixing concentrated bacillus subtilis bacterial liquid, concentrated saccharomycete bacterial liquid and concentrated bacillus mucilaginosus bacterial liquid in proportion so as to prepare compound concentrated bacterial liquid; and adding the compound concentrated bacterial liquid to a solution containing glyphosate and heavy metal ions, uniformly stirring the mixture, allowing the mixture to stand for 4-5 hours to generate precipitates, and washing, filtering and drying the precipitates to obtain minerals which are phosphate and carbonate. The result shows that the compound concentrated bacterial liquid is capable of effectively degrading the glyphosate and mineralizing the heavy metals to form the stable phosphate and carbonate minerals.

Description

technical field [0001] The invention belongs to the interdisciplinary science and technology in the fields of microbiology and environmental science, and relates to a microbial method for mineralization and restoration of heavy metal ions based on glyphosate degradation. Background technique [0002] In recent years, heavy metal pollution has become increasingly serious, the area of ​​pollution has continued to expand, the degree of pollution has continued to deepen, and the degree of harm has continued to increase, causing economic losses to increase year by year. At the same time, the extensive use of the pesticide glyphosate, and the accumulation of glyphosate residues year by year, have caused secondary pollution of soil and lakes contaminated by heavy metals, posing a huge safety hazard. At present, 26.67 million hectares of arable land in China have been polluted by heavy metals and glyphosate residues to varying degrees, and the polluted arable land accounts for 20% o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10C09K17/00C09K101/00
Inventor 钱春香詹其伟
Owner SOUTHEAST UNIV
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