Microbial method for remedying aromatic hydrocarbon-heavy metal ion complex pollution

A heavy metal ion and complex pollution technology, applied in the field of microbiology and environmental science, can solve the problems of high energy consumption, unsuitable for large areas, complicated operation, etc., achieve stable mineral properties, significant repair effect, and slow down the greenhouse effect. Effect

Active Publication Date: 2015-11-11
SOUTHEAST UNIV
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Problems solved by technology

Traditional restoration methods mainly use physical methods and chemical methods, but they are not suitable for large-scale pollu

Method used

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  • Microbial method for remedying aromatic hydrocarbon-heavy metal ion complex pollution
  • Microbial method for remedying aromatic hydrocarbon-heavy metal ion complex pollution
  • Microbial method for remedying aromatic hydrocarbon-heavy metal ion complex pollution

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[0036] (1) Weigh 5g of peptone, 3g of beef extract, 5g of NaCl, 15g of agar, MgSO 4 Dissolve 0.4g in 1000ml of deionized water, and control the pH to 7, sterilize at 125°C for 30 minutes, take it out and wait for cooling; inoculate Pseudomonas putida into the cooling medium solution, shake culture at 30°C, shake The frequency is 170r / min, and the culture time is 24h; centrifuge the cultivated bacterial liquid at high speed for 10min, the speed of the centrifuge is 8000rpm, and the temperature is 4°C. Remove the nutrients in the upper medium, and add 100mL of deionized water to make a concentrated bacterial liquid.

[0037] (2) Weigh 18g of agar and dissolve it in 1000ml of wort at 5°C, sterilize at 125°C for 30 minutes, take it out and wait for cooling; inoculate the yeast into the cooling medium solution, and culture at 30°C with shaking at a frequency of 170r / min, culture time 24h; Centrifuge the cultured bacterial solution at high speed for 10min, the speed of the centrifu...

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Abstract

The invention discloses a microbial method for remedying aromatic hydrocarbon-heavy metal ion complex pollution. The method comprises the steps: respectively inoculating Pseudomonas putida, saccharomycete and Bacillus mucilaginosus to corresponding culture media for culture, so as to prepare concentrated microbial solutions with the thallus concentration of 10<6> to 10<7>/mL; uniformly mixing the Pseudomonas putida concentrated microbial solution, the saccharomycete concentrated microbial solution and the Bacillus mucilaginosus concentrated microbial solution proportionally, so as to prepare a compounded concentrated microbial solution; and adding the compounded concentrated microbial solution into a solution containing aromatic hydrocarbons and heavy metal ions, uniformly stirring the compounded concentrated microbial solution and the solution, carrying out standing for 4-5 hours, and washing, filtrating and drying precipitates after the precipitates are produced, thereby obtaining carbonate Zn(CO3)(OH)6.H2O. Proven by results, the compounded concentrated microbial solution can be used for effectively degrading the aromatic hydrocarbons and mineralizing the heavy metal ions so as to form stable mineral carbonates. Compared with physical methods and chemical methods, the method disclosed by the invention has the advantages of low cost, remarkable effect, environment-friendliness, no secondary pollution and good ecological compatibility.

Description

technical field [0001] The invention belongs to the interdisciplinary science and technology in the fields of microbiology and environmental science, and relates to a microbial method for repairing aromatic hydrocarbon-heavy metal ion compound pollution. Background technique [0002] In recent years, with the transformation and development of cities and the adjustment of industrial structure, the existence of chemical parks in cities and suburbs has seriously restricted the development of cities, and the overall relocation of chemical parks has become a current hot issue. After the relocation of the chemical industry park, the soil and water bodies polluted by multiple pollution sources need to be restored ecologically, and aromatic hydrocarbon-heavy metal compound pollution is one of the problems. Traditional remediation methods mainly use physical and chemical methods, but they are not suitable for large-scale pollution remediation due to high energy consumption, large inv...

Claims

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Application Information

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IPC IPC(8): C02F3/34B09C1/10
Inventor 钱春香詹其伟
Owner SOUTHEAST UNIV
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