Terpene-rich mycelium culture medium of Antrodia antrodia and its application
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A culture medium and technology of terpene-rich bacteria, applied in the field of biotechnology, can solve the problems of short supply and high price of fruiting bodies of Antrodia camphorata
Active Publication Date: 2018-07-13
FUJIAN AGRI & FORESTRY UNIV
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[0003] In order to solve the current situation that Antrodia antrodia fruiting bodies are expensive and in short supply in the market, the present invention uses glucose, maltose, peptone, KH 2 PO 4 , MgSO 4 , V B1 , Juncao Ganoderma lucidum water extract, Juncao Ganoderma lucidum alcohol extract and Juncao Ganoderma lucidum lees water extract were selected as the screening factors, and the medium formula and application that can significantly increase the content of triterpenoids in Antrodia antrodia mycelia were selected
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Embodiment 1
[0039] The Antrodia camphorata mycelium culture medium of this embodiment is prepared according to the following ratio: glucose 10 g, maltose 4.2 g, peptone 15 g, MgSO 4 1g, KH 2 PO 4 0.6 g and 0.5 g of water extract of Ganoderma lucidum were dissolved in 1000 mL of water to adjust the pH value to 5.5, and each 100 mL was dispensed into 250 mL Erlenmeyer flasks, sterilized at 121°C for 20 min, and cooled for later use.
[0040] Culture method: culture at 28°C, 120 rpm, and dark for 15 days.
[0041] After measurement, the content of triterpenes in the mycelium was 35.67 mg·g -1 .
Embodiment 2
[0043] The Antrodia camphorata mycelium culture medium of the present embodiment is prepared according to the following proportions: glucose 100 g, maltose 42 g, peptone 15 g, MgSO 4 10g, KH 2 PO 4 6 g, 5 g of water extract of Ganoderma lucidum were distilled in 10 L of water, and the pH value was adjusted to 5.5. Each 1 L was dispensed into Erlenmeyer flasks, sterilized at 121°C for 20 min, and cooled for later use.
[0044] Culture method: culture at 28°C, 120 rpm, and dark for 15 days.
[0045] After measurement, the content of triterpenes in the mycelium was 38.98 mg·g -1 .
Embodiment 3
[0047] The Antrodia camphorata mycelium culture medium of the present embodiment is prepared according to the following proportions: glucose 500 g, maltose 210 g, peptone 75 g, MgSO 4 50g, KH 2 PO 4 30 g, 25 g of Ganoderma lucidum water extract in 50 L of water, adjust the pH value to 5.5, pack each 5 L into a small fermenter, sterilize at 121°C for 20 min, stir and ventilate internally after inoculation for later use.
[0048] Culture method: culture at 28°C, 120 rpm, for 15 days.
[0049] After measurement, the content of triterpenes in the mycelium was 43.98 mg·g -1 .
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Abstract
The invention provides an antrodia cinnamomea terpene-rich mycelium culture medium and an application and belongs to the field of biological science and technology. The best antrodia cinnamonea mycelium culture medium formula is prepared through Placket-Burman design, climbing experiment design and response surface design and optimization and comprises components as follows: 10.32 g*L<-1> of glucose, 42.58 g*L<-1> of maltose, 15.37 g*L<-1> of peptone, 1.12 g*L<-1> of MgSO4, 0.63 g*L<-1> of KH2PO4 and 0.55 g*L<-1> of a lucid ganoderma water extract, the inoculum quantity is 3%, and after antrodia cinnamonea mycelium is cultured for 15 days, the content of triterpene in the mycelium reaches 36.84 mg*g<-1>. Accordingly, the antrodia cinnamomea terpene-rich mycelium culture medium can be used for increasing the content of triterpene in the antrodia cinnamonea mycelium in the fluid culture medium in practical industrial production.
Description
technical field [0001] The invention relates to a terpene-rich mycelium culture medium of Antrodia camphorata and its application, belonging to the field of biotechnology. Background technique [0002] Fungi are the second largest group of organisms in the world, mainly divided into two categories: microorganisms and macrofungi (food and medicinal fungi). Antrodia Cinnamomea ( Antrodia cinnamomea ) is a rare medicinal fungus species unique to Taiwan, which belongs to Basidiomycota, Polyporales, Fomitopsidaceae, and Thinpora ( Antrodia ), first found growing on rotting hollow cinnamon trees ( Cinnamomum kanehirai ) inner wall, the early camphor tree was considered to be the only host of Antrodia camphorata. Antrodia camphorata tastes bitter, and studies have confirmed that it contains a large amount of triterpenoids, phenols, polysaccharides and other substances, which can be used to treat allergies, anti-inflammation, anti-oxidation, anti-tumor, liver protection, and im...
Claims
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