A kind of enterococcus faecium spray drying protective agent and spray drying method
A drying protectant, Enterococcus faecium technology, applied in the field of spray drying protective agent and spray drying of Enterococcus faecium, can solve the problems of high production cost, high price of trehalose, reduce the mortality rate of Enterococcus faecium, etc., and achieve low production cost , the effect of high survival rate
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Embodiment 1
[0013] The enterococcus faecium spray drying protection agent provided by the invention is composed of the following raw materials in parts by weight: 4-7 parts of soybean peptone, 5-7 parts of sorbitol, 3-6 parts of maltodextrin, and 1-3 parts of sucrose.
[0014] Enterococcus faecium spray drying method comprises the following steps:
[0015] ① Take the Enterococcus faecium concentrate prepared by liquid fermentation, add the above-mentioned Enterococcus faecium spray-dried protective agent, and mix evenly; the amount of the protective agent added is 13-23% of the mass of the bacterial concentrate;
[0016] ②Spray-dry the mixed bacterial liquid in step ①. The air inlet temperature in the spray dryer tower is 150°C~155°C, the temperature inside the tower is 80°C~95°C, the vacuum degree is -0.01 MPa ~-0.02MPa, and the outlet air temperature is 70°C~85°C.
[0017] The water content of the product after spray drying is less than 10%.
Embodiment 2
[0019] The enterococcus faecium spray drying protective agent provided by the invention is composed of the following raw materials in parts by weight: 50 kg of soybean peptone, 60 kg of sorbitol, 50 kg of maltodextrin, and 20 kg of sucrose.
[0020] Enterococcus faecium spray drying method comprises the following steps:
[0021] ①Separate 1,200 kg of liquid fermentation liquid containing Enterococcus faecium through a centrifuge to obtain 1,000 kg of bacterial concentrate; take a sample of 100 g to detect the content of live bacteria: 40 billion per gram;
[0022] ② Add 1000 kg of bacteria concentrate obtained in step ① to the mixing tank and add the above-mentioned Enterococcus faecium drying protectant and mix well;
[0023] ③Spray-dry the bacteria mixed liquid in step ②, the air inlet temperature in the spray dryer tower is 153°C, the tower inner temperature is 85°C, the vacuum degree is -0.02MPa, and the outlet air temperature is 80°C; 380 kg of spray-dried products are ob...
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