A strain of bacteriophage that can enter the blood through oral administration and its application

Abstract

The invention belongs to the field of bioengineering, and provides a bacteriophage capable of entering blood through oral administration and application of the bacteriophage. The bacteriophage monomer is a colibacteriophage; the Latin name is E.coli phage; the bacteriophage is named as Bp4; the bacteriophage has broad-spectrum sterilization capability on escherichia coli; the bacteriophage Bp4 is preserved in the general microbiology center of China Committee for Culture Collection of Microorganisms; the preservation date is 10th June, 2014; the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.9248. The bacteriophage has the strong cracking effect on the escherichia coli; bacteriophage sources are provided for industrial bacteriophage production, chicken colibacillosis prevention and control and environment sterilization, so that the severe escherichia coli viruses in a chicken farm are controlled. The bacteriophage can be used for industrial production, and the host bacterial escherichia coli specific amplification is realized. The bacteriophage can be used as a safe biological sterilization agent for environment sterilization. The bacteriophage can be used as biological antibiotic substitute to be applied to disease control in the chicken farm.

Description

Technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a phage that can be taken orally into the blood and its application. Background technique [0002] Bacteriophage is a type of virus that infects bacteria, which can be isolated from natural environmental samples such as sewage or feces. Bacteriophages have strict host specificity. Escherichia coli bacteriophages only invade E. coli, and have no infection effect on human, animal, and plant cells. Therefore, bacteriophages are not infectious to humans, animals and plants, and they will not pollute the environment, and have high safety. After the phage comes into contact with the corresponding host bacteria, it will trigger the adsorption and intracellular proliferation process of the phage, and release more phage through the lysis of the host bacteria. Bacteriophages can gain a large amount of their own amplification by killing specific host bacteria in the environment through...

AI Technical Summary

Problems solved by technology

[0003] Many broiler farms adopt the free-range breeding mode on the ground. Colibacillosis often occurs when broilers are raised to about 20 days old. Antibiotic treatment can only temporarily control the symptoms and cannot completely cure them. Continuously occur in the middle, bring great economic loss to the breeding industry

Antibiotic treatment often faces the problem of bacterial drug resistance. Many clinical isolates of Escherichia coli are resistant to multiple antibacterial drugs at the same time; broiler chickens with colibacillosis will excrete pathogenic Escherichia coli through feces, contaminate litter, and increase The limitation of ground disinfection during the free-range breeding of broilers on the ground makes E. coli a large number in the environment and increases the chance of broiler chickens being infected with E. coli disease

[0004] The phage disinfection test has been reported, but the phage that can enter the blood and persist for 14 hours after oral administration, and the phage can also have obvious disinfection effect on the total number of bacteria and E. coli in chicken manure has not been reported.

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