A method for preparing bacteriophage Bdellovibrio preparation by enrichment culture

A technology of phage Vibrio and enrichment culture, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of limited lysis ability and insignificant application effect of various pathogenic bacteria

Active Publication Date: 2021-09-28
福建九为生物技术有限公司
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Problems solved by technology

The above-mentioned Bdellovibrio preparations used in the production of Bdellovibrio preparations are all single Bdellovibrio strains obtained after purification as bacterial species, and there are usually multiple pathogenic bacteria in the aquaculture water body at the same time, and a single Bdellovibrio strain is effective against a variety of pathogenic bacteria. The cracking ability of germs is limited, so there are problems such as the application effect is not obvious

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  • A method for preparing bacteriophage Bdellovibrio preparation by enrichment culture

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Embodiment 1

[0028] A kind of enrichment culture method prepares the method for phage Bdellovibrio preparation, concrete steps are as follows:

[0029] Step 1: Isolation of host bacteria: Take 0.1 ml of water 20 cm below the water surface of seawater shrimp ponds in the middle stage of culture, inoculate it into TCBS Vibrio selective medium, spread it evenly, and place it in a constant temperature incubator at 35°C for upside-down culture 24 hours; the blue-green colonies that appear are Vibrio parahaemolyticus; pick a single colony of Vibrio parahaemolyticus and inoculate it continuously on a TCBS plate for 3 to 5 times, until the microscopic observation shows that the bacteria have the same shape and the same colony shape Bacterial colonies, inoculated in NB test tubes and cultured for 24 hours, are the host bacterial strains of Vibrio parahaemolyticus in this preparation;

[0030] The cultivation conditions of the TCBS Vibrio selective medium are as follows: the controlled temperature i...

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Abstract

The invention relates to a method for preparing a phage Bdellovibrio preparation by an enrichment culture method, which belongs to the field of biotechnology. The invention relates to the enrichment culture of various Bdellovibrio species, the culture of host bacteria and the production method of biological preparations. It is characterized in that the Bdellovibrio system naturally formed in the aquaculture water body cultivated by the enrichment culture method is the source of the Bdellovibrio species of the biological preparation, and the pathogenic Vibrio in the aquaculture water body is purified, cultivated, centrifuged, and inactivated Afterwards, it is used as a source of host bacteria for the production of Bdellovibrio preparations to produce a biological preparation containing a variety of Bdellovibrio. The Bdellovibrio preparation produced by this method has the characteristics of high activity, rapid action, wide cracking of pathogenic bacteria in the water body and the like because it contains a variety of Bdellovibrio.

Description

technical field [0001] The invention belongs to the technical field of microbial sterilization, and in particular relates to a method for preparing a bacteriophage Bdellovibrio preparation by an enrichment culture method. Background technique [0002] At present, the method of eliminating pathogenic bacteria in aquaculture water mainly uses various disinfectants and antibiotics to disinfect and purify the water body. This method can quickly kill pathogenic bacteria in the water body, but also kills beneficial microorganisms in the water body. Destroy the micro-ecological system of the water body. The long-term use of disinfectants and antibiotics has led to an increase in the resistance of pathogenic bacteria year by year, and the use of disinfectants and antibiotics cannot effectively control pathogenic bacteria in the end. [0003] In recent years, the use of beneficial microorganisms to control pathogenic microorganisms in aquaculture has attracted more and more attentio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/63
CPCC12N1/20
Inventor 文传敬谢荔朋
Owner 福建九为生物技术有限公司
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