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A semi-bionic enzyme method for extracting total flavonoids from persimmon leaves and its application

A technology of semi-bionic enzyme method and total flavonoids, which is applied in the field of extracting total flavonoids from persimmon leaves by semi-bionic enzyme method, can solve the problems of environmental pollution, large consumption of organic solvents, etc., and achieve high extraction rate, improved extraction rate, and mild extraction conditions Effect

Active Publication Date: 2021-12-28
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the extraction methods of flavonoids include hot water extraction, enzymatic extraction, ultrasonic-assisted extraction, etc., but there are problems such as large consumption of organic solvents and environmental pollution.
The semi-bionic enzyme method is a new type of environmentally friendly and efficient extraction method, and there is no report on the extraction of flavonoids from persimmon leaves by this method

Method used

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  • A semi-bionic enzyme method for extracting total flavonoids from persimmon leaves and its application
  • A semi-bionic enzyme method for extracting total flavonoids from persimmon leaves and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The method for extracting the total flavonoids of persimmon leaves by semi-bionic enzyme method (selection of extraction compound enzyme) comprises the following steps:

[0026] 1) Take the crushed persimmon leaves, add disodium hydrogen phosphate-citric acid buffer solution as the leaching agent according to the ratio of 1g:8mL of solid to liquid, adjust the pH to 2.0, fully shake evenly, and filter in a water bath at 70°C for 2 hours.

[0027] 2) Add disodium hydrogen phosphate-citric acid buffer solution to the filter residue in step 1), adjust the pH to 3.5, add the extraction complex enzyme, shake fully and evenly, and filter after 2 hours in a water bath at 60°C; the amount of the enzyme is the mass of persimmon leaves 0.6%.

[0028] The extraction compound enzyme was purchased from Ningxia Hersbit Biotechnology Co., Ltd., including β-glucanase, cellulase, xylanase and pectinase, wherein the activities of various enzymes are: β-glucanase Carbohydrase≧3 million u / ...

Embodiment 2

[0033] The method for extracting the total flavonoids of persimmon leaves by semi-bionic enzyme method (selecting pectinase) comprises the following steps:

[0034] 1) Take the crushed persimmon leaves, add disodium hydrogen phosphate-citric acid buffer solution as the leaching agent according to the ratio of solid to liquid 1g:10mL, adjust the pH to 2.0, shake fully and evenly, and filter in a water bath at 70°C for 2 hours.

[0035] 2) Add disodium hydrogen phosphate-citric acid buffer solution to the filter residue in step 1), adjust the pH to 3.5, add an enzyme that destroys plant cell walls, shake fully, and filter after 2 hours in a water bath at 60°C; the amount of the enzyme is 0.6% of mass.

[0036] 3) Add disodium hydrogen phosphate-citric acid buffer solution successively to the filter residue in step 2) as the extracting solution, adjust the pH value to 7.0, mix well, reflux extraction at 70° C. for 2 hours, and filter.

[0037] 4) Add disodium hydrogen phosphate-...

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Abstract

The invention discloses a method and application for extracting total flavonoids from persimmon leaves by a semi-bionic enzyme method, belonging to the technical field of biomedicine. The invention combines the semi-bionic method and biological enzymes to extract the total flavonoids in persimmon leaves, and sets different pH gradients to achieve the purpose of simulating the operation and absorption of medicines in the body. Compared with the semi-bionic method or enzymatic method alone, the method has the advantages of high extraction rate, mild extraction conditions, simple operation, friendly environment, and is suitable for large-scale industrial production. The total flavonoids from persimmon leaves extracted by the method has good α-glucosidase inhibitory activity.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method and application of extracting total flavonoids from persimmon leaves by a semi-bionic enzymatic method. Background technique [0002] Persimmon (Diospyros kaki Linn.f) is also known as vermilion fruit. It belongs to the persimmon family. Originally produced in the Yangtze River Basin, it is now widely cultivated in the south of the Yellow River. At present, my country is the country that produces the most persimmons in the world. Persimmon leaves are the fresh or dried leaves of the persimmon tree. Materia Medica newly records it: "bitter in taste, cold in nature, non-toxic", "specially enters the lung meridian". Persimmon leaves can be used to make tea, and Japanese people have the habit of drinking persimmon leaf tea. It is a kind of fitness drink, which contains tannins similar to tea leaves. It is fragrant after brewing. Persimmon leaf tea is a n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30A61K36/44A61P3/10
Inventor 郭增军薛璇玑赵雅韵陈子章陈建王希颖
Owner XI AN JIAOTONG UNIV
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