Antistreptolysin O immunological turbidimetry reagent and preparation method thereof

A streptolysin and immunoturbidimetric technology, which is applied in biological testing, material inspection products, measuring devices, etc., can solve the problems of reagent storage, reagent transportation or storage, and the laboratory cannot provide enough refrigeration space, etc. , to achieve the effect of reducing operation steps and operation time

Inactive Publication Date: 2015-11-11
NANJING PERLONG MEDICAL EQUIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the antistreptolysin O detection kits used in the domestic and foreign markets for turbidimetric detection are liquid double reagents; since freezing and thawing will affect the performance of R2 in the reagent, the product will be inactivated, and there is no There are freeze-dried reagents, so the transportation or storage of reagents in the northern region in winter has caused major problems; at the same time, more and more professional departments of hospitals or primary hospitals hope that manufacturers can provide POCT reagents and instruments, so that they can be tested as soon as possible timely diagnosis and treatment of patients; among them, the ready-to-use POCT reagents are especially favored by doctors, but the ready-to-use immunoturbidimetric reagents occupy a large space, and the laboratory cannot provide enough refrigerated space to store the reagents, so clinical It is urgent to solve the transportation and storage problems of immunoturbidimetric reagents
[0005] At the same time, due to the mutual interference between reagents, double reagents have been used clinically, and the problem of single reagent has not been solved; single reagent will make the operation steps simpler and reduce time

Method used

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  • Antistreptolysin O immunological turbidimetry reagent and preparation method thereof
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  • Antistreptolysin O immunological turbidimetry reagent and preparation method thereof

Examples

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Embodiment 1

[0028] A preparation method of an anti-streptolysin O immunoturbidimetric kit, the preparation steps of which are:

[0029] 1) Adding 20% ​​sucrose and 0.1% glycine to the liquid R1 and R2 reagents respectively; wherein, the R1 reagent is a PEG buffer, and the R2 reagent is latex coupled with a human anti-streptolysin O polyclonal antibody;

[0030] 2) Mix the above-mentioned liquid reagents R1 and R2 evenly and put them in the freezer overnight, then turn on the freeze-vacuum dryer to freeze-dry for 20 hours, seal the film and store at -20°C for 1 month in a container.

[0031] Then the above-mentioned anti-streptolysin O immunoturbidimetric kit is detected, and the detection steps are as follows:

[0032] 1. Turn on the instrument to warm up for 15 minutes. Before measuring the sample, place the reagent calibration card in the designated area of ​​the specific protein analyzer, follow the calibration operation instructions of the instrument, and then perform the sample test ...

Embodiment 2

[0047] A preparation method of an anti-streptolysin O immunoturbidimetric kit, the preparation steps of which are:

[0048] 1) Add 10% trehalose to the liquid R1 reagent, add 2% mannitol and 0.1% PVP mixture to the liquid R2, wherein the liquid R1 reagent is PEG buffer, R2 reagent is streptolysin O coupled colloidal gold;

[0049] 2) Freeze the above-mentioned liquid R1 and R2 reagents respectively, take them out and freeze them, turn on the freeze vacuum dryer, freeze them for 20 hours, seal them, and place the membranes at room temperature for 6 months;

[0050] 3) Finally, pack and fix in the container.

[0051] Then the above-mentioned anti-streptolysin O immunoturbidimetric kit is detected, and the detection steps are as follows:

[0052] 1. Turn on the instrument to warm up for 15 minutes. Before measuring the sample, place the reagent calibration card in the designated area of ​​the specific protein analyzer, follow the calibration operation instructions of the instru...

Embodiment 3

[0066] A preparation method of an anti-streptolysin O immunoturbidimetric kit, the preparation steps of which are:

[0067] 1) Mix the liquid R1 reagent and the liquid R2 evenly, wherein the liquid R1 reagent is a PEG buffer, and the R2 reagent is colloidal gold coupled with streptolysin O;

[0068] 2) Add 10% glycerol and fructose mixture to the above-mentioned liquid reagents R1 and R2 to form and freeze, turn on the freeze vacuum dryer, freeze for 10 hours, seal the film, and place it in a 37-degree refrigerator for 15 days;

[0069] 3) Finally, pack and fix in the container.

[0070] Then the above-mentioned anti-streptolysin O immunoturbidimetric kit is detected, and the detection steps are as follows:

[0071] 1. Turn on the instrument to warm up for 15 minutes. Before measuring the sample, place the reagent calibration card in the designated area of ​​the specific protein analyzer, follow the calibration operation instructions of the instrument, and then perform the sa...

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Abstract

The invention discloses an antistreptolysin O immunological turbidimetry reagent. The reagent comprises a freeze-dried reagent R1 and R2, which are fixed in a same container. The freeze-dried reagent R1 and R2 are prepared by adding liquid reagent R1 and R2 into an excipient and a protective agent and then freeze-drying the mixture; wherein the liquid reagent R1 is a buffering solution containing PEG, the liquid reagent R2 is latex coupled with antistreptolysin O or colloidal gold coupled with antistreptolysin O. The operation of the provided reagent for detection is convenient, time is saved, at the same time, the reagent is convenient for transportation and preservation, moreover, the reagent can be transported in cold areas, and the reagent stability is improved.

Description

technical field [0001] The invention relates to the field of immune turbidimetry, in particular to an anti-streptolysin O immune turbidimetric reagent and a preparation process thereof. Background technique [0002] A metabolite produced by hemolytic streptococcus can dissolve red blood cells, so this product is named "O" hemolysin (SLO). After the human body is infected with group A hemolytic streptococci, it will produce specific antibodies. Anti-streptolysin (ASO) is a specific immunoglobulin present in serum. [0003] The determination of streptolysin antibody (ASO) is one of the important indicators for the diagnosis of group A hemolytic streptococcus (A chain) infection. After the body is infected with group A streptococci due to pharyngitis, tonsillitis, scarlet fever, erysipelas, pyoderma, rheumatic fever, etc., it can produce streptolysin O antibodies, namely "Anti-Streptolysin O (ASO)". A significant increase in the level of this antibody is closely related to ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/569
CPCG01N33/6854G01N33/56944
Inventor 张春东孙珊黄宝福谷万俊韩东成淳林
Owner NANJING PERLONG MEDICAL EQUIP
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