Preparation method and application of Liuwei Dihuang pills

A technology of Liuwei Dihuang Pills and Dihuang, which is applied in the field of preparation of Liuwei Dihuang Pills, can solve the problems of large dosage for patients, affecting application, rough process, etc.

Active Publication Date: 2015-11-18
NANJING TONGRENTANG PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no report on the use of resin technology in the extraction and preparation of Liuwei Dihuang Pills. However, the method of decocting in water is rough, backward, and has many impurities, which leads to excessive dosage for patients and is inconvenient to take, which seriously affects the quality of the medicine. products in clinical application

Method used

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  • Preparation method and application of Liuwei Dihuang pills

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Take Rehmannia glutinosa 120g, Cornus officinalis 60g, Cortex paeonol 45g, Chinese yam 60g, Poria cocos 45g, Alisma 45g, add 8 times the amount of distilled water to extract twice, each time for 1.5 hours, combine the extracts, concentrate to 0.4ml / g Crude drug, add ethanol to make the alcohol content reach 80%, let it stand for 8 hours, filter with suction, take the supernatant, pass the medicinal liquid through a D1010 type macroporous adsorption resin column, wash with water until colorless, elute with 90% ethanol, collect and wash Remove 10BV of liquid and evaporate to dryness to obtain part A; Concentrate the washing liquid after passing through the macroporous resin, adjust the pH value of the medicinal liquid to 2, then apply 732 type cation exchange resin, wash with water until colorless, and use 40% ammonia water containing 3% Elution with ethanol, collect the eluent, and evaporate to dryness to obtain part B; finally, concentrate the washing solution...

Embodiment 2

[0011] Example 2: Liuwei Dihuang Pills inhibit the proliferation of mouse embryonic fibroblast NIH3T3 experimental research data

[0012] 1 Experimental materials

[0013] 1.1 The cell line used in the experiment: mouse embryonic fibroblast NIH3T3, the laboratory cell bank of Nanjing Tongrentang Pharmaceutical Co., Ltd., DMEM+10%FBS conventional culture.

[0014] 1.2 Experimental drug: Research drug: Liuwei Dihuang Wan of the present invention: prepared according to the method of Example 1. Medicinal solution stock solution: Weigh 100 mg of Liuwei Dihuang Pills, dissolve in 5 ml of absolute ethanol, filter through a 0.2 μm filter, aliquot into 500 μldoff tubes, store at -20°C, and filter absolute ethanol through a 0.2 μm filter for use in the control group.

[0015] 1.3 Experimental reagents: DMEM (GIBCO Cat.No.12100-061Lot.No.758137); fetal bovine serum (Zhejiang Tianhang Biotechnology Co., Ltd. Lot.No.100419); NaHCO3 (Shanghai Jiuyi Chemical Reagent Co., Ltd. Cat. No.11810...

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Abstract

The invention belongs to the field of traditional Chinese medicine preparations and particularly relates to a preparation method of Liuwei Dihuang pills. The Liuwei Dihuang pills are made from 120g of prepared rehmannia root, 60g of common Macrocarpium fruit, 45g of root-bark of tree peony, 60g of common yam rhizome, 45g of Indian bread and 45g of rhizome of oriental water plantain. The Liuwei Dihuang pills are prepared with macroporous resin, cation exchange resin and anion exchange resin, so that contents are greatly increased and less usage is required. The invention further provides application of the Liuwei Dihuang pills in the preparation of medicaments for inhibiting proliferation of mouse embryonic fibroblast NIH3T3.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine preparations, in particular to a preparation method and application of Liuwei Dihuang Pills. Background technique [0002] Liuwei Dihuang Wan is recorded in the standard of the Ministry of Health. It is made from 120g Rehmannia glutinosa, 60g Cornus officinalis, 45g paeonol, 60g Chinese yam, 45g Poria cocos, and 45g Alisma. Nourishing yin and tonifying kidney. For deficiency of kidney yin, dizziness and tinnitus, soreness of the waist and knees, hot flashes due to bone steaming, night sweat and nocturnal emission. In the prior art, there is no report on the use of resin technology in the extraction and preparation of Liuwei Dihuang Pills, but the method of decocting in water is rough, backward, and has many impurities, which leads to excessive dosage for patients and is inconvenient to take, which seriously affects the quality of the medicine. products in clinical applicatio...

Claims

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Application Information

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IPC IPC(8): A61K36/8945A61K9/20A61P1/14A61P35/00
Inventor 周勤文陈家进
Owner NANJING TONGRENTANG PHARM CO LTD
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