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Construction method of Pothos chinensis HPLC fingerprint of and HPLC fingerprint

A fingerprint and construction method technology, applied in the field of quality inspection of traditional Chinese medicine, can solve problems such as weak quality control of shikanzi medicinal materials, lack of quality evaluation system, difficulty in achieving effective quality control of shikanzi, and achieve the effect of ensuring stability

Active Publication Date: 2015-11-25
GUANGDONG SECOND TRADITIONAL CHINESE MEDICINE HOSPITAL (GUANGDONG PROVINCE ENG TECH RES INST OF TCM) +1
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Problems solved by technology

[0003] But at present, there is no quality standard for the medicinal materials of shikanzi, and the quality control of the medicinal materials of shikanzi is very weak. It only includes routine inspections such as microscopic identification, lacks a perfect quality evaluation system, and it is difficult to achieve effective control of the quality of shikanzi.

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  • Construction method of Pothos chinensis HPLC fingerprint of and HPLC fingerprint
  • Construction method of Pothos chinensis HPLC fingerprint of and HPLC fingerprint
  • Construction method of Pothos chinensis HPLC fingerprint of and HPLC fingerprint

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[0033] see Figure 1 ~ Figure 2 , the present invention will be further elaborated below by specific embodiment.

[0034] Waterse2695-2489 high performance liquid chromatography (USA): PAD detector, quaternary gradient pump, Empower2 data processing software system; MilliporeAdvantageA10 ultrapure water system (USA); MettlerToledoXS205DU electronic analytical balance (Switzerland); KQ5200DE numerical control Ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); HH-8 digital display constant temperature water bath (Jintan Keyan Instrument Co., Ltd.).

[0035] Use feruloyl tyramide (self-made, purity ≥ 99.5%), syringic acid (self-made: purity ≥ 99.0%), vanillic acid (batch number: X-030-140801, purchased from Chengdu Ruifensi Biotechnology Co., Ltd.); for liquid phase Methanol was chromatographically pure (Merck); liquid phase water was ultrapure water; extraction water was purified water made by the preparation room of Guangdong Second Hospital of Traditional Chinese M...

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Abstract

The present invention discloses a construction method of Pothos chinensis HPLC fingerprint of and an HPLC fingerprint based on high-performance liquid chromatography. The fingerprint has 16 common peaks with the following relative retention time tg: 0.178min, 0.268min, 0.309min, 0.354min, 0.411min, 0.488min, 0.507min, 0.633min, 0.730min, 0.922min, 1.000min, 1.318min, 1.326min, 1.525min, 1.544min, and 1.579min. Through the presence or absence of common peaks in the fingerprint, a comprehensive quality evaluation of Pothos chinensis can be carried out, so as to ensure the stability of medicinal quality and effectiveness and safety of clinical medication.

Description

technical field [0001] The invention relates to the technical field of quality detection of traditional Chinese medicines, in particular to a method for constructing an HPLC fingerprint of ponzu and its HPLC fingerprint. Background technique [0002] Mandarin is the dried whole herb of Pothoschinensis (Raf.) Merr., a plant of the genus Pothoschinensis (Raf.) Merr. in the family Araceae. Lime mandarin is flat in nature and light in taste. It has the effects of dispelling wind and dampness, promoting blood circulation and dispersing blood stasis, eliminating stagnation and relieving cough. It is often used in the Yao area to treat bruises and rheumatic bone pain. The curative effect is remarkable. Due to the complex composition of traditional Chinese medicine, it is difficult to accurately evaluate the quality of traditional Chinese medicine only by a single index. Traditional Chinese medicine fingerprint refers to the chromatogram or spectral spectrum of a certain type or se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 孙冬梅罗文汇钟鸣毕晓黎刘布鸣邱宏聪陈昭赖茂祥胥爱丽江洁怡
Owner GUANGDONG SECOND TRADITIONAL CHINESE MEDICINE HOSPITAL (GUANGDONG PROVINCE ENG TECH RES INST OF TCM)
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