Compositions and methods for improving the health of aquatic animals

A technology for aquatic animals and compositions, applied in the directions of microorganism-based methods, biochemical equipment and methods, methods using bacteria, etc., which can solve the problems of aquatic animal death, infection of aquatic animals, yield loss, etc.

Inactive Publication Date: 2015-12-02
NOVOZYMES AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another major challenge across the industry is yield loss due to pathogenic microorganisms that infect and cause death or disease in aquatic animals.

Method used

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  • Compositions and methods for improving the health of aquatic animals
  • Compositions and methods for improving the health of aquatic animals
  • Compositions and methods for improving the health of aquatic animals

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0296] Example 1: Inhibition potential in vitro

[0297] Competitive inhibition of a panel of Bacillus candidate strains was evaluated against a range of targeted pathogenic bacterial strains including Serratia rubidaea, Vibrioharveyi, Fischeri Vibriofischeri, Vibrio alginolyticus, and Aeromonas hydrophila. Selected Bacillus strains were quantitatively evaluated by their ability to inhibit the growth of "lawns" of target bacterial pathogens (as mentioned in Table 1) growing on petri dishes. This is best quantified by using defined "wells" (holes) inserted into the agar into which a defined amount of viable culture of the test Bacillus strain is placed.

[0298] Diffusion assays were performed when the test strain Bacillus candidates were grown in nutrient broth that was autoclaved and cooled prior to strain inoculation. For tests involving inhibition of Vibrio species, the nutrient broth was supplemented with 1.5% (w / v) sodium chloride. Bacillus strains were grown in nutrient...

example 2

[0303] Example 2: Quorum Sensing Inhibition

[0304] Vibrio fischeri (MJ-1) was used as the indicator because its coloration depends on an intact quorum sensing pathway. The greater the effect of the QSI on V. fischeri, the less fluorescence was observed. Quorum-sensing compounds allow bacteria to "communicate" and affect phenotypes such as colouration, motility, pathogenicity, and Vibrio colonization in shrimp gills or gut. Thus, quorum sensing inhibition is, for example, the mode of action against Vibrio colonization in the gut.

[0305] The Bacillus candidate and indicator bacterium Vibriofischeri (MJ-1) were grown according to the method described in Example 1. The results are provided in Table 2. Inhibition of pigmented areas, but not total cell growth, was scored as QSI positive and its major diameter was measured for semi-quantitative results. Total growth inhibition of V. fischeri is expressed as "VC" for vibriocidal drugs.

[0306] Table 2: Relative areas of QS...

example 3

[0308] Example 3: Survival of spores in extruded fish feed pellets

[0309] For a Bacillus strain to be effective in aquafeed itself, the inoculated spores must survive at effective levels during the pelleting process. Typically, optimal fish feed values ​​are obtained when feed pellets are passed through nozzles at high temperatures (100°C to 120°C) before drying and subsequent use in feeding, while other animal feed types are well formed at 85°C-95°C .

[0310] Microbial spores were assessed for viability potential during the standard fish food pelleting process. Commercial fish feed is generally produced using a high temperature extrusion process, in which a feed meal is extruded through a heated nozzle under a defined temperature and pressure profile. The tests were carried out using a Werner & Pfleiderer 37 screw extruder through a 2.7 mm dye nozzle with horizontal mixing at the temperatures described in Table 3.

[0311] Prior to extrusion, the microorganisms were add...

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Abstract

The present invention relates to compositions and methods for increasing the health of aquatic animals comprising one or more selected bacterial strains.

Description

field of invention [0001] The present application discloses methods and compounds involving one or more bacteria for improving the well-being, general condition or health of aquatic animals. Background of the invention [0002] Commercial fishing (the practice of harvesting fish and other seafood from wild fisheries for commercial profit) has raised concerns about the sustainability of wild fish stocks. As such, aquaculture has become a viable option for large-scale commercial fishing. Aquaculture, or aquaculture, is the cultivation of freshwater and saltwater stocks under controlled conditions and has been found to be beneficial because they reduce the pressure on commercial fishing from wild fisheries and also reduce human intake of toxins such as , heavy metals such as mercury), these toxins are often found in wild-caught fish such as tuna. Despite the positive effects of aquaculture on wild fisheries, challenges remain for the aquaculture sector. In particular, mainta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18C12N1/20
CPCC12R1/07A23K1/003A23K1/009A23K1/188C12N1/20C12R1/09C12R1/10C12R1/11C12R1/12C12R1/125A23K10/18A23K50/80A23K40/25C12R2001/09C12N1/205C12R2001/12C12R2001/11C12R2001/10C12R2001/07C12R2001/125A23K40/20A61K9/0056A61K35/741A61K35/742
Inventor D·德拉霍斯C·蒙克L·V·科弗德
Owner NOVOZYMES AS
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