123 results about "Aquatic fauna" patented technology

The invention belongs to the technical field of gene engineering, and discloses a CRISPR / Cas9 delivery system and application thereof. The CRISPR / Cas9 delivery system is obtained by loading the CRISPR / Cas9 system with the fluorinated polyethyleneimine, and the fluorinated polyethyleneimine can deliver the CRISPR / Cas9 carrier into cells, has excellent transmembrane delivery efficiency, and can efficiently mediate the CRISPR / Cas9 system to enter target cells. The CRISPR / Cas9 delivery system is applied to large-scale breeding of aquatic animals, the use safety is high, the biocompatibility is excellent, and the delivery system has excellent gene editing efficiency and has wide application prospects in the field of gene editing breeding.

The invention provides a health monitoring method, device and system based on aquatic animal sequestration, and belongs to the technical field of data processing technology and aquatic product healthy cultivation, and the method comprises the steps: obtaining a physical feature parameter vector of to-be-monitored aquatic animal sequestration, the physical characteristic parameter vector is determined by at least one of particle size distribution information, hydrodynamic parameters, dissolution characteristics, color characteristics and unit time increment of the aquatic animal excrements to be monitored; respectively inputting the physical characteristic parameter vector obtained at each monitoring moment into a health monitoring model to obtain instantaneous health state information output by the model; forming a time sequence by a plurality of pieces of instantaneous health state information, performing feature analysis on the time sequence, and determining a final health state; and continuously monitoring, analyzing and displaying the health state of the aquatic animal to be monitored. The invention further provides a device and a system which are used for better implementing the method to obtain a more effective health monitoring result and improve the accuracy of aquatic animal health monitoring.

The invention discloses an aquatic ecosystem exposure device for semi-volatile organic pollutants, which comprises a light control thermal insulation cabin, an aquatic ecosystem is arranged in the light control thermal insulation cabin, the aquatic ecosystem is arranged in a glass fish tank in the light control thermal insulation cabin, and the aquatic ecosystem comprises an aquatic animal exposure area and an aquatic plant exposure area. A fish manure collecting system is arranged at the bottom of the aquatic animal exposure area, a sediment collecting system is arranged at the bottom in the light-control heat-preservation cabin body, and a water sample collecting system, a target object adding and acid-base regulating device, a gas collecting and purifying device and an air exposure system are arranged outside the light-control heat-preservation cabin body in a communicating mode. The problems that in the prior art, the exposure condition of semi-volatile organic pollutants in an aquatic ecosystem cannot be effectively simulated, multiple types of samples such as gas, water, sediments, organisms and organism excrement cannot be comprehensively collected, and experiment conditions cannot be accurately controlled are solved.

The invention discloses a three-dimensional water ecological restoration method based on an ecological system, and the method specifically comprises the following steps: S1, carrying out the construction of a temporary dam for a river channel of a construction area, and carrying out the water ecological operation and water level reduction for the construction area; impurity cleaning is conducted on the river channel area after the water level is lowered; s2, in-situ remediation of the bottom mud: scattering a bottom material modifier in the river channel area after the sundries are cleared to complete the in-situ remediation of the bottom mud; submerged plants are planted; s3, after the submerged plants are planted, an ecological water purification barrier and a biological grid are installed, and water purification is conducted; s4, emergent aquatic plant buffer zone planting, microorganism regulation and control and aquatic animal putting are conducted on the two sides of the river channel, a three-dimensional water ecological restoration network is constructed, and water ecological restoration is completed. Through the synergistic effect of the ecological water purification barrier and the biological grid, rapid and high-strength physical adsorption and microbial degradation of pollutants are achieved, meanwhile, ecological niche is built, and recovery of biological diversity is achieved.

The invention relates to the technical field of seawall ecological transformation, and discloses a slope type seawall inner slope ecological transformation method which comprises the following steps: selecting a seawall section suitable for ecological transformation according to three first-level indexes, namely a seawall shoreline foundation, hydrology and water quality conditions and surrounding environment conditions; constructing a pool in the selected seawall section according to the terrain; constructing a pool base; and constructing a plant community in the water pool according to the life type, the phenological period and the salt tolerance of the plant. According to the method, a proper transformation section can be selected in combination with various environmental factors of a transformation area, then a corresponding water pool is constructed in combination with the landform, then a proper ecological material is selected to construct a substrate, and different plant communities are constructed in the water pool according to the characteristics of different plants. A natural-imitated seawall ecological space is built, the flood prevention and tide blocking functions of the seawall are guaranteed, the hygrophytes, aquatic plant communities and planktons can be utilized, an inhabiting and foraging environment is built for aquatic animals and hydrophilic birds, and the ecological service function of the seawall is improved.

The invention belongs to the technical field of black and odorous water treatment, and particularly relates to a method for repairing and treating rural black and odorous water. According to the invention, aquatic plants, aquatic animals and microbial communities are configured in the system, and hydrodynamic optimization is combined, so that an underwater forest ecological system with self-maintenance capability is constructed, biological self-purification of the black and odorous water body and long-acting stability of the ecological system are realized, and the system is suitable for black and odorous water body treatment of rural rivers and ponds. The ecological balance of a water body is maintained through closed-loop feedback of excessive plankton, enhancement of fish ingestion, promotion of plant growth by excrement and absorption of excessive nutritive salt, meanwhile, oxygen is supplied through photosynthesis of submerged plants, and zero external energy consumption is achieved by combining stepped oxygen transfer without external aeration. In addition, the raw material of the carrier is derived from agricultural wastes, so that the treatment cost is reduced, and the recycling of rural solid wastes can be effectively promoted.

The invention provides an aquatic animal source trypanosomes visual detection primer probe set, a kit and a detection method, and belongs to the technical field of parasite detection. The invention provides a detection reagent for aquatic animal source trypanosomes. The detection reagent comprises a recombinase polymerase amplification primer pair and a probe which are designed according to an SSU rDNA gene conserved region of the aquatic animal source trypanosomes. Aiming at SSU rDNA sequence conserved regions of multiple aquatic animal trypanosomes, the invention develops a detection method and a kit based on recombinase polymerase amplification combined with a lateral flow test strip technology, and the detection method and the kit have the advantages of strong specificity, high sensitivity, short time consumption, result visualization and the like, and have low requirements on detection sites, instruments and personnel; the method is suitable for rapid detection of trypanosomes in basic laboratories and farms, and has important significance on prevention and control of trypanosomes of aquatic animals.

Apparatuses and systems suitable for separating aquatic animals, preferably large marine animals, from a fishing line, wire, and / or hook are disclosed herein. In an embodiment, the apparatus includes a base, which is affixable to an extender, having a return aperture and seat for receiving one or more blades to form a cutting edge along the return aperture; a cover rotatably fastened to the base; a locking point to affix the base and the cover; wherein the apparatus is configurable such that, if one or more straight-edge blades is inserted into the seat, no blade tip extends beyond a perimeter of the base and the cover. Also described are systems incorporating the apparatuses elsewhere described, further possessing an extender and one or more blades. Further, described herein are methods of using any of the apparatuses and / or systems elsewhere described to separate a marine animal from a line or hook.

The application discloses a kind of water ecological restoration plant community management method and system based on neural network, method includes: based on SOFM neural network, water body stratification detection is carried out to target water area, and stratification result is obtained;According to stratification result, dynamic hydrological data sampling is carried out to each water layer using water body monitoring equipment, and the sampling result of each water layer is obtained;According to aquatic plant distribution data and aquatic animal distribution data, corresponding biological information and water layer natural ecological environment data are called to construct target database;Based on BP neural network, according to target database, determine the dynamic change rule of target water area within a predetermined time;Judge whether the dynamic change rule meets the preset restoration target, according to the judgment result, based on ecological restoration principle, calculate the plant species and corresponding quantity required for each water layer, and obtain the calculation result;According to the calculation result, the plant community of target water area is managed using plant management equipment.

Provided are chitosan-RNA particles comprising low molecular weight chitosan and ssRNA or dsRNA, as well as compositions and cultured aquatic animals comprising the chitosan-RNA particles, and methods of use thereof in the treatment or prevention of microsporidia infection in aquaculture, the ssRNA or dsRNA is partially complementary, bound, or at least 90% identical to the mRNA target of a pathogenic microsporidium parasite in cultured fish or crustacean.

The invention relates to a device for analyzing and sorting animals and preferably aquatic animals, comprising: a housing (9); a tunnel (1) extending through the housing (9), comprising tunnel walls (4, 5), the tunnel (1) being positioned within the housing (9) and configured to guide an individual animal through the device, the tunnel (1) comprises: two or more image capturing units (6) positioned within the housing (1) configured to capture images of the tunnel (1), wherein each image capturing unit (6) comprises an image capturing device to capture images of the tunnel (1); configured for capturing images of at least one section of the tunnel (1), wherein said image capturing units (6) are provided laterally behind the tunnel walls (4, 5) on opposite lateral sides of the tunnel (1), said tunnel walls (4, 5) on the lateral sides being transparent; and illumination means (10) configured to provide illumination at said at least one section of the tunnel (1), said upper and / or lower tunnel wall (4, 5) being transparent.

The invention discloses and provides a combined box-type ecological anti-scour protection foot for river bank foot protection, the ecological anti-scour protection foot can improve the anti-scour capacity of a river bank foot and can replace vertical anti-scour measures such as adding concrete toe feet or sheet piles on the upstream face of the bank foot or a retaining wall, the appearance of the ecological anti-scour protection foot is in a box shape, and the ecological anti-scour protection foot is in a box shape. A peripheral box body frame is formed by combining reinforced concrete prefabricated parts, strip-shaped gaps are formed between the prefabricated parts, the box body is filled with small stone blocks, and the surfaces of the stone blocks are pressed by concrete prefabricated blocks. The prefabricated parts are spliced through embedded steel bars in the preformed holes and concrete in the pouring holes, and then the prefabricated parts, the filled small stone blocks and the surface layer ballasting prefabricated blocks jointly form a box-shaped porous river bank foot scour prevention system, and the ecological scour prevention protection foot can achieve the purposes of reinforcing the front toe of a river retaining wall and improving the scour resistance of the retaining wall. On the other hand, the ecological anti-scour protection foot is of a porous structure, a suitable habitat can be created for aquatic animals in the river channel, and the biodiversity of the river channel can be improved.

A process, system, and use of the system are disclosed, which enable the successive growth of aquatic animals and algae within the same aquaculture pond and optionally reduction of infestation species in ponds bottom solids of the aquaculture pond.

The invention relates to the technical field of aquatic animal breeding, in particular to a feeding device for shrimp breeding, which comprises a shell, a charging barrel is arranged at the top of the shell, a discharge port is formed in the bottom of the charging barrel, a scattering pipe is movably arranged on the shell, a connecting pipe is arranged between the discharge port and the scattering pipe, an air blower is arranged on the scattering pipe, and the connecting pipe is connected with the air blower. The steering mechanism is arranged between the shell and the material scattering pipe, and the angle adjusting mechanism is arranged between the shell and the material scattering pipe. Through integrated treatment of crushing, conveying and throwing, the whole flow of bait from storage to throwing is optimized, and the problems of caking and non-uniform throwing existing in a traditional throwing mode are effectively solved; the special motion control of the feed scattering pipe ensures the optimal distribution state of the bait in the water body, not only meets the ingestion requirements of prawn groups, but also avoids the deposition of residual bait to the maximum extent, effectively improves the utilization rate of the bait, and reduces the risk of water pollution.

The utility model relates to the technical field of slope protection structures, in particular to a slope type ecological slope protection structure which is characterized in that a base block and a cover block are oppositely arranged, guide beams are arranged between the base block and the cover block in parallel, and the two ends of each guide beam are fixedly connected with the base block and the cover block respectively; the adjacent guide beams, base blocks and cover blocks form a frame, the area, close to the base blocks, of the frame is an ecological area, and the area, close to the cover blocks, of the frame is a bank protection area. A plurality of ecological blocks are arranged in the ecological area from low to high, and a plurality of bank protection blocks are arranged in the bank protection area from low to high; the ecological block comprises an outer wall, the outer wall protrudes towards the side deviating from the guide beam, so that an ecological cavity is formed in the side, facing the guide beam, of the ecological block, an inlet hole is formed in the outer wall, opening holes are formed in the periphery of the inlet hole, and the inlet hole and the opening holes are communicated with the ecological cavity. The ecological blocks with the ecological cavities are designed, and the ecological blocks are communicated with the environment, so that the inhabitation space of aquatic animals in non-flood seasons is guaranteed, and the inhabitation space of aquatic animals in high water level in flood seasons is also guaranteed.

A feed additive pack includes an antioxidant component with astaxanthin dispersed within the antioxidant component. A fish feeds includes a base feed and a feed additive pack. The antioxidant component comprising particles having a particle size volume distribution with D80 in a range of 140 to 200 micrometers. Methods of making feed additive packs and fish feeds, and methods of feeding aquatic animals are also provided.

The objective is to provide formulations or feeds that improve the tolerance of aquatic animals to environmental stress, and to provide methods for raising aquatic animals using these. [Solution] An environmental stress tolerance enhancer for aquatic animals is provided, containing a predetermined nucleic acid as an active ingredient. The weight-average molecular weight of the nucleic acid is in the range of 5,000 to 50,000, and the absorbance of the nucleic acid at a wavelength of 420 nm is 3.0 or less. This environmental stress tolerance enhancer is incorporated into a feed for aquatic animals. These agents or feeds are administered to raise or manage the growth of aquatic animals.

The invention discloses a seasonal waterflooding beach arbor willow trunk insertion afforestation method, and belongs to the technical field of trunk insertion afforestation. Comprising the following steps: preparing an arbor willow trunk; selecting a planting area at the seasonal flooded beach land; soil preparation is conducted on the planting area to form planting ridges, and planting holes are punched in the planting ridges according to the preset planting density; preparing a sleeve, and inserting the sleeve and the tree willow trunk into the planting hole, so that the tree willow trunk is sleeved with the sleeve; in the dry season of the current year, the casing pipe is dismantled, and hilling and treading are conducted. According to the method, the sleeve is used as temporary physical protection, so that a contact channel between aquatic organisms and seedling bases is effectively blocked, and the situation that the bases of the tree willow trunks are gnawed by aquatic animals to cause plant death is avoided. In addition, by arranging the sleeve, the tree willow trunk can be prevented from being directly scoured by water flow, and lodging and breaking of the tree willow trunk are avoided. After the tree willows grow and are formed, the casing pipes are removed in time, and inhibition of long-term wrapping on tree trunk growth is avoided.

The present application belongs to the technical field of black and odorous water treatment, and particularly relates to a method for repairing and treating black and odorous water in rural areas. The present application configures aquatic plants, aquatic animals and microbial communities through a system, combines with water power optimization, and constructs an underwater forest ecological system with self-maintenance capability, so as to realize biological self-purification and long-term stability of the ecological system of the black and odorous water, and is suitable for the treatment of black and odorous water in rural rivers and ponds. Through the closed-loop feedback of excess plankton -> enhanced fish feeding -> excrement promoting plant growth -> absorbing excess nutrients, the ecological balance of the water body is maintained, and the oxygen supply of the photosynthesis of submerged plants is combined with the step-by-step oxygen transfer, so that external aeration is not needed, and zero external energy consumption is realized. In addition, the carrier raw material of the present application is derived from agricultural waste, which reduces the treatment cost and effectively promotes the resource utilization of rural solid waste.

The application provides a multi-stage adaptive fish activity intelligent regulation method, system and chip, and relates to the technical field of aquatic animal management. The method comprises the following steps: classifying the species and activity state of fish to be processed according to a preset knowledge base, and determining regulation parameters; setting a regulation environment according to the regulation parameters, and regulating the activity of the fish to be processed according to the regulation environment, and collecting activity signals of the fish to be processed, water temperature signals, water quality signals and gas signals of the regulation environment through a sensing chip during the regulation process; determining the activity state value of the fish to be processed during the regulation process through a processing chip according to the activity signals; and adjusting the regulation environment through a master control chip according to the activity state value. The application solves the problems of single regulation dimension, poor environmental adaptability and insufficient stage adaptability in the prior art.

This invention provides a tiered biological ecological filter bed purification system using functionalized porous media, relating to the field of environmental remediation technology. The system comprises a primary purification zone, a secondary purification zone, a tertiary purification zone, an underwater deep purification zone, and an aquatic animal habitat, sequentially connected along the water flow direction. Each purification zone is arranged in layers from top to bottom and is respectively equipped with soilless hydroponic plants, large-particle-size functionalized porous media, medium-particle-size adsorption-type functionalized porous media, small-particle-size catalytic-type functionalized porous media, a biological filter, and biomimetic aquatic plants. This allows the water flow to undergo interception, retention, adsorption, and deep biochemical purification processes at each stage, improving the graded purification capacity of non-point source pollution runoff and reducing the risk of clogging.

The invention discloses an aquatic animal toxicology analysis platform based on behavior simulation. Comprising the following steps: collecting aquatic animal videos and water quality data, extracting behavior characteristics and calculating a behavior deviation sequence, synchronously serializing water quality parameters, establishing a behavior-water quality correlation model by adopting a grey correlation method, constructing a dynamic characteristic set to train a GAN generation model, generating simulation characteristics, secondarily identifying abnormal behaviors based on the simulation characteristics, and finally identifying the abnormal behaviors. And generating a toxicological report by combining the water quality parameters. According to the method, the space-time correlation mechanism of the behavior deviation and the water quality parameters is established, dynamic simulation and prediction recognition of behavior characteristics are achieved through the GAN model, multi-dimensional quantitative evaluation of the water quality parameters is achieved, the toxicology evaluation level and efficiency are remarkably improved, and the method is suitable for the fields of ecological toxicology research and aquatic organism protection.

The present invention relates to the technical field of prevention and control of aquatic animal diseases, and specifically relates to a nanocomposite for a nervous necrosis virus, a preparation method therefor, and the use thereof. Provided in the present invention is a nanocomposite consisting of poly(lactic-co-glycolic acid), polyethyleneimine and a siRNA. Also provided in the present invention are a preparation method for the nanocomposite and the use thereof in the preparation of a drug for improving the survival rate of fish eggs infected with a nervous necrosis virus. The present invention constructs a vector for a capsid protein of the nervous necrosis virus by means of simulating the nervous necrosis virus and expresses same in cells, and screens out a siRNA that can effectively inhibit the expression of the viral capsid protein, thus constructing the nanocomposite for the nervous necrosis virus. The nanocomposite can block NNVs at the embryonic phase (fertilized eggs), thereby improving the survival rate of fish fry, and further opening up new possibilities for the treatment of early stage diseases in fish fry. The method is innovative in the field of artificial breeding of aquatic animals and provides new ideas for virus prevention and control in the field of aquaculture.

The present application belongs to the field of environmental remediation technology, and particularly relates to a method for ecological remediation of heavily polluted water body with bottom mud. The method for ecological remediation of heavily polluted water body with bottom mud comprises the following steps: S1: putting heavy metal remediation agent into the water body; S2: putting slow-release type bottom mud remediation agent into the water body; S3: sequentially putting algae-controlling microorganism, photosynthetic bacteria and EM bacteria, and plankton into the water body; S4: planting aquatic plants according to water depth; planting emergent plants in the area with water depth less than 0.5 m, and planting submerged plants in the area with water depth greater than or equal to 1.5 m; and S5: when the coverage rate of aquatic plants in the water body is greater than or equal to 80%, putting aquatic animals into the water body. In the present application, slow-release type bottom mud remediation agent and heavy metal remediation agent are used to reduce the pollution of bottom mud, organic matter, etc., the transparency of the water body is then improved after reduction, aquatic plants are then planted, and finally aquatic animals are put into the water body, so as to construct a water ecological system and achieve the purpose of ecological remediation of the water body.

In the aquatic organism invasion risk assessment system provided in the embodiments of the present application, the target risk factor required in the risk assessment process of the specific aquatic organism invading the specific water area is obtained through the assessment and calculation of the risk factor assessment terminal and the server, the risk assessment value of the specific aquatic organism invading the specific water area is obtained through the assessment and calculation of the target risk factor by the risk value assessment terminal and the server, and the risk degree of the specific aquatic organism invading the specific water area can be known through the risk assessment value. Therefore, the aquatic organism invasion risk assessment system provided in the embodiments of the present application can assess the risk degree of the specific aquatic organism invading the specific water area, and by analogy, the risk degree of the specific aquatic organism invading any water area other than the specific water area can also be assessed, and risk avoidance measures are taken according to the risk assessment result.

This invention provides a method for constructing an ecological lake wetland aquatic plant and animal community system. The method includes dividing the lake to be restored into two zones according to water flow direction and terrain. Then, bottom sediment improvement, planting of submerged plants, addition of amendments, and algae-controlling zooplankton are carried out in each zone. Ecological floating islands are constructed and emergent plants are planted. Aquatic animals are introduced, followed by routine management. Water purification devices containing EHBR membrane modules are installed near the lake inlet and in areas with higher terrain. This constructs an ecological lake wetland aquatic plant and animal community, achieving long-term ecological restoration of the lake. Furthermore, the introduction of EHBR membrane modules can reduce organic pollutants in the lake at lower temperatures, significantly extending the restoration time and ensuring that the lake's water quality remains stable for an extended period.

A self-contained underground growing apparatus (500) including a container (502) configured to be positioned underground, an opening into the apparatus configured to allow human access from a surface of the ground and including a releasable hatch (514), a fluid container (714) configured to contain an aqueous solution, multiple internal containers (530) configured to enable growing of at least one of aquatic animal life, plants, or fungi and configured to enable the aqueous solution to flow therethrough, a rebreather system, a heat transfer system (760), and a computerized control device (722).

The invention provides a lake water ecological system restoration method based on staged biological regulation and control, and belongs to the field of water environment treatment and water ecological restoration. According to the method, directional putting and regulation of aquatic animal communities are performed in three stages according to the recovery process of submerged plants: in the early regulation stage, carnivorous fishes are put to inhibit breeding of benthic omnivorous fishes; a middle-stage regulation and control stage: introducing benthonic animals to cooperatively control the quality of algae biomass and improve the transparency of the water body; in the later regulation and control stage, herbivorous fishes are put to promote plant tillering, lateral branch development and root system downward growth and expansion, and the substrate fixing capacity is enhanced; according to the method, the aquatic animals with the specific function class groups are sequentially put in stages, directional regulation and control of the aquatic animal community structure are effectively achieved, the planting success rate of the submerged plants is increased, growth of the aquatic plants is promoted, water transparency is improved, and a stable water ecosystem with the self-purification capacity is constructed.

The invention relates to the technical field of artificial lakes, and discloses a water resource six-in-one original ecological control integration which comprises an anti-seepage structure, and the artificial lake is provided with a lake bottom area, a revetment area and a shore top area; the anti-seepage structure comprises a bearing base layer, a waterproof blanket and a backfill soil layer. Aquatic animals, net algae biological populations and aquatic plants are arranged in the artificial lake, and the lake bottom area is provided with an iron wire cage and is filled with a planting soil layer and a plurality of stones; concrete blocks are arranged in the revetment area, and a plurality of pores and inhabiting cavities for aquatic animals to inhabit are formed in the concrete blocks; the inhabiting cavities penetrate through the peripheries of the concrete blocks to form a plurality of side openings, the side openings of the adjacent concrete blocks are aligned and communicated, and cross elastic nets are arranged in the inhabiting cavities; a plurality of trees are planted on the bank top area; on the basis of effectively preventing leakage of water resources, a complete aquatic ecosystem is constructed, so that ecological prevention and control of the water resources are realized.