Construction method and applications of Shanxi mature vinegar liquid-phase fingerprint

A fingerprint spectrum and construction method technology, which is applied to the fingerprint spectrum construction method of Shanxi aged mature vinegar and its application field in quality control, can solve the problems of narrow detection range, cumbersome processing process, unable to fully reflect the inherent quality of Shanxi aged mature vinegar, and the like, To achieve the effect of short time-consuming and simple pre-processing

Active Publication Date: 2015-12-16
阳泉市裕盛源农产品开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing studies have either focused on its aroma, that is, volatile components, or on its organic acid components. Not only is the sample pretreatment process cumbersome, but more importantly, the detection range is narrow, which cannot fully reflect the internal quality of Shanxi mature vinegar.

Method used

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  • Construction method and applications of Shanxi mature vinegar liquid-phase fingerprint
  • Construction method and applications of Shanxi mature vinegar liquid-phase fingerprint
  • Construction method and applications of Shanxi mature vinegar liquid-phase fingerprint

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Take 1ml of Shanxi mature vinegar sample (sample No. 1), filter it with a 0.22 μm microporous membrane, and take the subsequent filtrate to obtain the test solution. The hydrophilic chromatographic column Synrgi4uHydro-RP80A (250×4.60mm) was used as the stationary phase, and the test was carried out according to the following liquid phase conditions:

[0033] Flow rate: 0.8mL / min; detection wavelength: 254nm; column temperature: 26°C; injection volume: 5μL; linear gradient elution; mobile phase: mobile phase A is acetonitrile, mobile phase B is formic acid with a volume fraction of 0.1% Aqueous solution, elution program: 0~5min, 0% phase A; 5~21min, 0%~6.4% phase A; 21~59min, 6.4%~30% phase A; 59~62min, 30%~0% phase A , 62 ~ 65min, 0% A phase.

Embodiment 2

[0035] Take 1ml of Shanxi mature vinegar sample (sample No. 1), filter it with a 0.22 μm microporous membrane, and take the subsequent filtrate to obtain the test solution. The hydrophilic column Synrgi4uHydro-RP80A (250×4.60mm) was used as the stationary phase, and the test was carried out according to the following liquid phase conditions:

[0036] Flow rate: 0.5mL / min; detection wavelength: 260nm; column temperature: 26°C; injection volume: 5μL; linear gradient elution; mobile phase: mobile phase A is acetonitrile, mobile phase B is formic acid with a volume fraction of 0.1% Aqueous solution, elution program: 0~5min, 0% phase A; 5~21min, 0%~6.4% phase A; 21~59min, 6.4%~30% phase A; 59~62min, 30%~0% phase A , 62 ~ 65min, 0% A phase.

Embodiment 3

[0038] Take 1ml of Shanxi mature vinegar sample (sample No. 1), filter it with a 0.22 μm microporous membrane, and take the subsequent filtrate to obtain the test solution. The hydrophilic column Synrgi4uHydro-RP80A (250×4.60mm) was used as the stationary phase, and the test was carried out according to the following liquid phase conditions:

[0039] Flow rate: 0.8mL / min; detection wavelength: 240nm; column temperature: 30°C; injection volume: 5μL; linear gradient elution; mobile phase: mobile phase A is acetonitrile, mobile phase B is phosphoric acid with a volume fraction of 0.1% Aqueous solution, elution program: 0~5min, 0% phase A; 5~21min, 0%~6.4% phase A; 21~59min, 6.4%~30% phase A; 59~62min, 30%~0% phase A , 62 ~ 65min, 0% A phase.

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Abstract

The present invention discloses a construction method and applications of a Shanxi mature vinegar liquid-phase fingerprint, and relates to the technical field of fingerprint construction. The construction method comprises: (1) taking 1 mL of Shanxi mature vinegar, and filtering by using a 0.22 [mu]m micro-pore filtration membrane for spare; and (2) sucking the filtered Shanxi mature vinegar, and determining through a high-pressure liquid chromatograph to obtain the chemical fingerprint. According to the present invention, the sample pre-treatment is simple and easy, the chemical components and the quality characteristics of the Shanxi mature vinegar can be completely reflected, the Shanxi mature vinegar liquid-phase fingerprint can be used for the quality control of the Shanxi mature vinegar and can further provide technical support of the protection of the Shanxi mature vinegar having the origin production region and the geographical indication.

Description

technical field [0001] The invention relates to the construction of fingerprints, in particular to a method for constructing fingerprints of Shanxi mature vinegar based on liquid chromatography technology and its application in quality control. Background technique [0002] Shanxi mature vinegar is a characteristic industry in Shanxi Province with a long history. Its unique five-character process of "steaming, fermenting, smoking, pouring and drying" makes it have a unique flavor of sour, mellow, fragrant and sweet. With the development of various analytical methods, identification methods and instruments, the systematic research on the chemical components of vinegar has gradually deepened. It has been reported that vinegar contains sugars, amino acids, acids, alcohols, aldehydes, esters, polyphenols, etc. Element. [0003] In recent years, the content of ligustrazine and total flavonoids has been added to the quality standard of Shanxi mature vinegar. However, the composi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 李震宇范玛莉邢婕张丽增秦雪梅
Owner 阳泉市裕盛源农产品开发有限公司
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