Tissue engineering endometrium and preparation method thereof

An endometrial and tissue engineering technology, applied in the field of tissue engineering medical biomaterials, can solve problems such as difficult regeneration, and achieve the effects of promoting proliferation and maturation, promoting fusion, improving cell activity and microenvironment

Inactive Publication Date: 2015-12-23
广东芙金干细胞再生医学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to regenerate the basal layer of the endometrium after injury, and the clinic is helpless for such patients

Method used

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  • Tissue engineering endometrium and preparation method thereof
  • Tissue engineering endometrium and preparation method thereof
  • Tissue engineering endometrium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Step 1. Preparation of intimal basal layer: take the third generation bone marrow mesenchymal stem cells and press 10 5 / cm 2 The density of BMSCs was seeded in the cell culture plate, and after the cells adhered, the culture medium A was replaced and cultured for 20 days, so that the bone marrow mesenchymal stem cells were induced to differentiate into intimal basal cells and formed a membranous structure; the composition of culture medium A was: Commercial high-glucose DMEM medium contains, fetal bovine serum 100ml / L, dexamethasone 10 -9 mol / L, β-glycerophosphate sodium 5mmol / L and ascorbic acid 37.5μg / mL;

[0027] Step 2. Preparation of endometrial functional layer: The fourth generation umbilical cord mesenchymal stem cells were prepared at 5 × 10 5 / cm 2 The density of umbilical cord mesenchymal stem cells was inoculated in the cell culture well plate, and after the cells adhered to the wall, the culture medium B was replaced to induce culture for 13 days, so th...

Embodiment 2

[0031] Step 1. Preparation of intimal basal layer: take the fourth generation bone marrow mesenchymal stem cells and press 5 × 10 5 / cm 2 The density of BMSCs was seeded in the cell culture plate, and after the cells adhered, the culture medium A was replaced and cultured for 10 days, so that the bone marrow mesenchymal stem cells were induced to differentiate into endometrial basal cells and form a membranous structure; the composition of culture medium A was: Commercial high-glucose DMEM medium contains, fetal bovine serum 100ml / L, dexamethasone 10 -8 mol / L, sodium β-glycerophosphate 18.5mmol / L and ascorbic acid 85μg / mL;

[0032] Step 2. Preparation of intimal functional layer: The third-generation umbilical cord mesenchymal stem cells were prepared at 2 × 10 5 / cm 2 The density of umbilical cord mesenchymal stem cells was inoculated in the cell culture plate, and after the cells adhered to the wall, the culture medium B was replaced to induce culture for 10 days, so that...

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Abstract

The invention provides a tissue engineering endometrium and a preparation method thereof. The tissue engineering endometrium comprises a functional layer and a basal layer. Compared with the prior art, the tissue engineering endometrium provided by the invention has the advantages that functional cells formed through performing induction culture on mesenchymal stem cells are adopted to respectively prepare cell sheets, the cell density is high, autocrine extracellular matrix is taken as a support, and respective biological properties can be played to a greatest extent. After recombination with collagen or hyaluronic acid, through induction culture of various hormone in vitro, the tissue engineering endometrium with a natural endometrium double-layer structure is formed, and can show characteristics of regular thickening, falling off, regenerating and the like under periodic hormone concentration variation after being implanted into a body, and can achieve functions of a normal endometrium.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering medical biomaterials, and in particular relates to a tissue-engineered endometrium with endometrial function and a preparation method thereof. Background technique [0002] Endometrium (uterine endome-trium) refers to the layer that constitutes the lining of the mammalian uterus. The endometrium responds to both estrogen and progesterone and therefore can change significantly with the sexual cycle (estrous cycle, menstrual cycle). Estrogen can cause uterine hypertrophy, and progesterone can induce special changes in the endometrium in early pregnancy, or change the nature of the endometrium, so that it has the ability to produce decidua. The endometrium covers the mucosa and consists of a mucosal epithelium with an underlying lamina propria. The mucosal epithelium is columnar epithelium, cuboidal epithelium or stratified columnar epithelium. When estrogen is secreted, each epithelial ...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/36A61L27/24A61L27/20
Inventor姚瑶
Owner广东芙金干细胞再生医学有限公司