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Pinus armandii Franch rosin extract, cosmetic adopting same as active ingredient and application of Pinus armandii Franch rosin extract

A technology of pine resin and extract, which is applied in Huashan pine resin extract and cosmetics and application fields using it as an active ingredient, can solve the problems of no activity, etc., and achieve the goal of improving resource utilization value, improving utilization function and added value Effect

Inactive Publication Date: 2015-12-30
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yet, do not see in the prior art from the report of Huashan pine resin extract and its activity in cosmetics or medicine

Method used

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  • Pinus armandii Franch rosin extract, cosmetic adopting same as active ingredient and application of Pinus armandii Franch rosin extract
  • Pinus armandii Franch rosin extract, cosmetic adopting same as active ingredient and application of Pinus armandii Franch rosin extract
  • Pinus armandii Franch rosin extract, cosmetic adopting same as active ingredient and application of Pinus armandii Franch rosin extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of Huashan pine resin extract:

[0026]Take 10 g of Pinus armandii Franch rosin, grind the sample into powder, soak in 25 times the amount of 85% ethanol (301-302 mL) for 24 hours, then collect the filtrate with a Buchner funnel, and repeat 3 times. The filtrate was distilled under reduced pressure with a rotary evaporator, the filtrate was concentrated, and the concentrated filtrate was dried at low temperature to obtain a crude extract. The crude extract was adsorbed and decolorized by macroporous resin (D101), and eluted with 95% ethanol. The eluate was concentrated, evaporated to dryness, and freeze-dried at low temperature to obtain 2.3 g of Huashan pine resin extract, with an extraction rate of 23%.

[0027] Alternatively, weigh 10 g of Huashan pine resin sample, grind the sample into powder, cold-soak with 20 times the amount of 95% ethanol for 18 hours, then collect the filtrate with a Buchner funnel, and repeat 5 times. The filtrate was distilled u...

Embodiment 2

[0029] Cytotoxicity test of Huashan pine resin extract: by MTS colorimetry, the CC of the sample was determined 50 (50%cytotoxicconcentration), that is, the drug concentration when producing toxicity to 50% of the cells, thereby determining the safe sample concentration for the following activity experiments.

[0030] Experimental method: on a 96-well cell culture plate, mix B16 cells with different concentrations of the drug solution to be tested, set 3 replicate wells, and set a blank control without drug at the same time, 37 ° C, 5% CO 2 After culturing for 24 hours, the cytotoxicity was detected by the MTS colorimetric method, and the OD value was measured by a microplate reader at a wavelength of 490 nm. Calculated to get CC 50 value.

[0031] Cell survival rate (%) = experimental well OD 490nm / blank well OD 490nm ×100%

[0032] Experimental results: The cell survival rate of Huashan pine resin extract is 100.760%. It can be clearly seen that Huashan pine resin extr...

Embodiment 3

[0034] HDFa collagen secretion promotion experiment: HDFa cells were mixed with different concentrations of drug solutions to be tested on a 12-well cell culture plate, and a blank control without drugs and a TGF-β positive control were set. 37°C, 5% CO 2 After culturing for 3 days, collect the cell culture supernatant and store it at -80°C; add MTS, and use the MTS colorimetric method to detect the OD value at 490nm; detect the secretion of collagen according to the method provided in the collagen ELISA kit, and measure it with a microplate reader OD value, the detection wavelength is 450nm. The increase rate of collagen secretion was calculated.

[0035] Cell survival rate (%) = experimental well OD 490nm / blank well OD 490nm ×100%

[0036] Collagen secretion increase rate (%)=(experimental well OD 450nm / cell viability / blank well OD 450nm -1)×100%

[0037] Experimental results: In the co-culture with human fibroblasts, Huashan pine resin extract (EC 50 =65.562μg / mL)...

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Abstract

The invention discloses Pinus armandii Franch rosin extract, a cosmetic additive adopting the same for realizing skin whitening, moisturizing and antioxidant activity and an application of the Pinus armandii Franch rosin extract in cosmetics. The extract is ethanol extract of Pinus armandii Franch rosin, has good moistening and moisture preserving functions as well as a skin metabolism improvement function and a skin barrier function and can be taken as a collagen secretion promoting activator for skin cosmetics. The Pinus armandii Franch rosin extract breaks through limitation of a traditional utilization mode of Pinus armandii Franch rosin and is a good raw material for herbal cosmetics.

Description

technical field [0001] The invention belongs to the field of cosmetics and medicine, and specifically relates to extracting a natural active substance from a widely used chemical raw material Huashan pine resin—Huashan pine resin extract, cosmetics or medicines using it as an active ingredient, and the preparation of Huashan pine extract It can be used in various skin cosmetics such as moisturizing, skin rejuvenation, anti-wrinkle, sunscreen, and whitening, as well as in the preparation of drugs for treating or preventing skin diseases. technical background [0002] Huashan pine (Pinusarmandii Franch), also known as white pine, five-beard pine, and fruit pine, is a plant in the genus Pinaceae. The young tree bark is gray-green or light gray, smooth, and cracks into square or rectangular thick pieces when it is old. The cones are green when young and light yellowish brown when mature; the apex of the seed scales is not recurved or slightly recurved; the navel of the scales is...

Claims

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Application Information

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IPC IPC(8): A61K36/15A61K8/97A61P17/00A61P23/00A61P29/00A61P31/10A61P39/06A61Q17/04A61Q19/00A61Q19/02A61Q19/08
Inventor 杨立新许刚刘爱忠裴盛基
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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