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A method for knocking down trim5alpha gene in cynomolgus monkeys

A technology of cynomolgus monkeys and genes, applied in the direction of genetic engineering, plant genetic improvement, botany equipment and methods, etc., to achieve excellent knockdown effect

Active Publication Date: 2019-11-08
广州高新区生物研究和实验发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • A method for knocking down trim5alpha gene in cynomolgus monkeys
  • A method for knocking down trim5alpha gene in cynomolgus monkeys
  • A method for knocking down trim5alpha gene in cynomolgus monkeys

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Embodiment 1

[0033] The invention provides a method for knocking down the TRIM5alpha gene in cynomolgus monkeys, the specific steps are as follows:

[0034]1)针对食蟹猴TRIM5alpha基因的mRNA确立靶序列;根据食蟹猴TRIM5alpha基因的mRNA序列(atggcttctggaatcctgcttaatgtaaaggaggaggtgacctgtcccatctgcctggaactcctgacagaacccctgagtctgcactgcggccacagcttctgccaagcgtgcatcactgcgaaccacaagaagtccatgctatacaaagaaggagagagaagctgccctgtgtgccggatcagttaccagcctgagaacatacagcctaatcggcatgtagccaacatagtggagaagctcagggaggtcaagttgagcccagaagaggggcagaaggttgatcactgtgcacgccatggagagaaactcctactcttctgtcaggaggacagcaaggtcatttgctggctttgtgagcggtctcaggagcaccgtggtcaccacactttcctcatggaggaggttgcccaggagtaccatgtgaagctccagacagctctggagatgctgaggcagaagcagcaggaagctgaaaagttggaagctgacatcagagaagagaaagcttcctggaagattcaaatagaccacgacaaaaccaacgtcttggcagattttgagcaactgagagagatcctggaccgggaggagagcaatgagctgcagaacctggagaaggagaaagaagacattctgaaaagtcttacaaagtctgaaacgaagatggtgcagcagacccagtacgtgagagagctcatctcagatctggagcatcggttgcaggggtcaatgatggagctgctgcagggtgtggatggcatcattaaaaggattgagaacatgaccttgaaga...

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Abstract

The invention discloses a method for knocking down TRIM 5 alpha (tripartite motif protein 5 alpha) genes of macaca fascicularis and belongs to the field of biotechnology. The method for knocking down TRIM 5 alpha genes of macaca fascicularis comprises steps as follows: 1), a target sequence targeted at mRNA of the TRIM 5 alpha genes of the macaca fascicularis is determined; 2), corresponding shRNA at each site is synthesized; 3), corresponding lentivirus is packaged; 4), embryonic fibroblast cells of the macaca fascicularis are transfected, and cells with the TRIM 5 alpha genes of the macaca fascicularis knocked down are obtained. The knockdown effect detection shows that the knockdown rate is 90% or above, and a solid foundation is laid for establishment of an animal model for AIDS (acquired immune deficiency syndromes).

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for knocking down TRIM5alpha gene in cynomolgus monkeys. Background technique [0002] TRIM5alpha (tripartite motif protein 5 alpha) is a member of the TRIM family and is an important restriction factor in mammalian cells. They restrict multiple viruses including HIV-1 (Humanimmunodeficiency virus type 1) in a species-dependent manner. A retrovirus replicates. The mechanism of TRIM5 restricting HIV-1 provides an important scientific basis for TRIM5-based biological therapy, drug development and vaccine design, and is also of great significance for the establishment of a more ideal non-human primate AIDS model. TRIM5 has four domains, including RING, B-BOX, Coiled-Coil and SPRY; among them, if RING is destroyed, the restrictive effect of TRIM5 on HIV can be partially eliminated, and if B-BOX is destroyed, the restrictive effect of TRIM5 on HIV can be completely e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/073C12N15/866
Inventor 杨世华马瑞瑞黄群山
Owner 广州高新区生物研究和实验发展有限公司
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