Application of TIMELESS genes used as targets to preparing medicines for treating cervical cancer

Abstract

The invention belongs to the field of biomedical technologies, provides a function of DNA (deoxyribonucleic acid) damage repair related genes for cervical cancer, and further relates to application of TIMELESS siRNA [small interfering RNA (ribonucleic acid)] and medicine compositions to treating cervical cancer. The medicine compositions comprise effective dose of siRNA nucleotide fragments and pharmaceutically acceptable excipients. The application has the advantages that obvious correlation between the gene TIMELESS expression level and the progress of the cervical cancer is confirmed by the aid of molecular biology means for the first time, and the TIMELESS genes are hardly expressed in normal cervical tissues; the siRNA with the TIMELESS genes used as targets at gene level is screened, and siRNA sequences capable of efficiently and specifically knocking down the TIMELESS expression are provided; the proliferation and clone formation ability of cervical cancer cells can be obviously inhibited by the TIMELESS siRNA, apoptosis and senescence of the cervical cancer cells can be promoted, and the cisplatin sensitivity of the SiHA (human cervical squamous carcinoma) cells can be promoted; expression of Cleaved Caspase-3 and p21 can be up-regulated by the siRNA, and accordingly the apoptosis and senescence of the cervical cancer cells can be ultimately promoted.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to the application of the TIMELESS gene as a target in the preparation of drugs for treating cervical cancer. Background technique [0002] Cervical cancer is the second most common malignancy in women and the fourth leading cause of cancer-related death in women worldwide. With the popularization of cervical cancer screening, the incidence of advanced cervical cancer has been significantly reduced. However, due to the large population base in China, there are still a large number of cervical cancer patients who need radical radiotherapy or supplementary radiotherapy and chemotherapy after surgery. Advanced cervical cancer and recurrence The prognosis of patients with cervical cancer is not ideal. It is very important to improve the curative effect of cervical cancer and improve the prognosis of patients by finding effective targeted therapy molecules to enhance the sensitivity o...

AI Technical Summary

Problems solved by technology

[0006] However, so far there is no literature reporting the role of abnormally high expression of TIMELESS in the occurrence and progression of cervical cancer, and TIMELESS siRNA can significantly inhibit the proliferation of cervical cancer cells, promote their senescence and apoptosis, and promote their sensitivity to cisplatin

No siRNA targeting TIMELESS has been used in the treatment of cervical cancer

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