Micro-fluidic chip for multi-object quantitative detection based on magnetic particle chemiluminescence

A microfluidic chip and quantitative detection technology, applied in the direction of chemiluminescence/bioluminescence, chemical instruments and methods, and analysis through chemical reactions of materials, etc., can solve the high cross-contamination rate of reagents and the large consumption of reagents and samples , low sensitivity and repeatability

Active Publication Date: 2016-01-20
SHENZHEN HUAMAIXINGWEI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these products use colloidal gold immunochromatography, which can achieve simultaneous detection of multiple targets to a certain extent, but these methods consume a lot of reagents and samples, have a high degree of signal interference, and have a high cross-contamination rate of reagents. The sensitivity is low, and the detection range needs to be further widened

Method used

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  • Micro-fluidic chip for multi-object quantitative detection based on magnetic particle chemiluminescence
  • Micro-fluidic chip for multi-object quantitative detection based on magnetic particle chemiluminescence
  • Micro-fluidic chip for multi-object quantitative detection based on magnetic particle chemiluminescence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1. Troponin I (cTnI), myoglobin (Myo), creatine kinase isoenzyme (CK-MB) joint detection method one

[0053] Three items of myocardial infarction, ie troponin I (cTnI), myoglobin (Myo) and creatine kinase isoenzyme (CK-MB), are detected simultaneously by using the method of the present invention. To complete the project test, the specific steps are as follows:

[0054] 1. Encoding of Magnetic Particle Groups

[0055] Three kinds of magnetic particles with a particle size of 2.0 μm and a magnetic content of 0.5%, 5%, and 30% were selected, respectively labeled 1, 2, and 3, which were respectively used for the detection of cTnI, Myo, and CK-MB.

[0056] 2. Antibody labeling:

[0057] 1) Enzyme-labeled antibody: Weigh 5 mg of HRP and dissolve it in 1 ml of distilled water to make a 5 mg / ml HRP solution, add 0.2 ml of newly prepared 0.1M NaIO to the solution 4 The solution was stirred at room temperature in the dark for 20 minutes; the solution was put into a ...

Embodiment 2

[0068] Example 2. Combined detection method for troponin I (cTnI), myoglobin (Myo), and creatine kinase isoenzyme (CK-MB)

[0069] Three items of myocardial infarction, ie troponin I (cTnI), myoglobin (Myo) and creatine kinase isoenzyme (CK-MB), are detected simultaneously by using the method of the present invention. Troponin I (cTnI), myoglobin (Myo) and creatine kinase isozyme (CK-MB). To complete the project test, the specific steps are as follows:

[0070] 1. Encoding of Magnetic Particle Groups

[0071] Select three kinds of magnetic particles whose magnetic content is 10%, but the particle size of the magnetic particles is 0.5 μm, 2.0 μm, and 5.0 μm, respectively, labeled 1, 2, and 3, respectively, corresponding to the detection of cTnI, Myo, and CK-MB .

[0072] 2. Antibody labeling:

[0073] 1) Enzyme-labeled antibody: Weigh 5 mg of HRP and dissolve it in 1 ml of distilled water to make a 5 mg / ml HRP solution, add 0.2 ml of newly prepared 0.1M NaIO to the solution...

Embodiment 3

[0084] Example 3: Magnetic Particle Size Screening

[0085] The particle size of magnetic microspheres is small, the specific surface area is large, and the surface contains active groups, so the coupling capacity is large, but the size of magnetic particles is too small to be conducive to magnet collection, so the magnetic particle size screening is carried out.

[0086] For other experimental conditions, refer to Example 2. The content of magnetic particles is 10%, and the size is determined according to the following scheme.

[0087] Six kinds of magnetic particle labels with particle sizes of 0.1 μm, 0.5 μm, 1 μm, 2 μm, 3 μm and 10 μm were selected to mark anti-troponin I (cTnI), myoglobin (Myo), creatine kinase isoenzyme (CK) -MB) antibodies for separate determinations of the three targets. The permanent magnet whose magnetic size has been optimized is used for detection, and the height and movement speed of the magnet are fixed.

[0088] The results of the separate det...

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Abstract

The invention relates to a micro-fluidic chip for multi-object quantitative detection based on magnetic particle chemiluminescence. The micro-fluidic chip is of a double-layer structure and comprises a cover plate (1) and a bottom plate (11), wherein an air pump (3), an air flow micro-channel (5), a sample adding hole (2), a sample liquid flowing channel (6), a first biomarker storage pool (4), a micro-mixer (7) and a transition area (10) on the cover plate (1) are connected in sequence. A filter (12), a reaction chamber (13), a cleaning area (14), a detection chamber (15) and a solution release channel (18) on the bottom plate (11) are connected in sequence, and the detection chamber (15) is connected with a luminous liquid storage pool (16) and a cleaning fluid storage pool (17) respectively through the solution release channel (18).

Description

technical field [0001] The invention relates to a microfluidic chip for quantitative detection of multiple targets based on magnetic particle chemiluminescence. The chip combines microfluidic technology and magnetic microsphere technology to quickly and conveniently realize multiple targets in the microfluidic chip. Quantitative detection of substances. The technical solution belongs to the field of biological analysis and detection. technical background [0002] In recent years, the field of bioanalysis technology has developed rapidly, and many important research directions have emerged. Microfluidic chip analysis technology is one of the most active ones, and has received extensive attention in both scientific research and application fields. As a new type of analysis and detection platform, microfluidic chips have the advantages of high throughput, integration, portability, easy operation, and low cost, and have been widely used in many fields. [0003] Magnetic micro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76B01L3/00
Inventor 范玉霞李泉
Owner SHENZHEN HUAMAIXINGWEI MEDICAL TECH CO LTD
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