Knoxia valerianoides tissue culture plant cultivation method

Abstract

The invention discloses a knoxia valerianoides tissue culture plant cultivation method. The method comprises the following steps: 1, preparation of a plantation furrow, i.e., successively laying a mixed plantation matrix with a thickness of 5 to 10cm and fine river sand with a thickness of 3 to 5cm on a furrow surface from bottom to top to form the plantation furrow, wherein the mixed plantation matrix is prepared by the following steps: spraying an A liquid on an organic waste for uniform mixing , carrying out complete fermentation and composting, and then spraying a B liquid for full uniform mixing; and 2), transplantation of tissue culture plants. Compared to the prior art, the transplanting survival rate and the final plant survival rate of the Knoxia valerianoides tissue culture plants obtained by use of the cultivation method provided by the invention are more than 95%, and the bottleneck problem of low artificial large-scale survival rate of Knoxia valerianoides tissue culture plants is effectively solved.

Description

technical field [0001] The invention relates to a method for cultivating red euphorbia tissue culture seedlings. More specifically, the present invention relates to a method for cultivating Euphorbia euphorbia tissue cultured seedlings which greatly improves the survival rate. Background technique [0002] Red Euphorbia (Knoxiavalerianoides Thorel.) is a perennial herbaceous plant of the genus Knoxiavalerianoides Thorel in the Rubiaceae family. It is distributed in the southern provinces and regions of my country. [0003] The medicinal part of Euphorbia euphorbia is the root, which has the effects of diuresis, detumescence, and dissipating stagnation. Bud Euphorbia's ecological characteristics), reuse can also treat mad syndrome (Yu Huimin, reusing red bud Euphorbia to treat 12 cases of mad syndrome); not only the main drug of Chinese patent medicine Zijinding, but also the production of Guilin Tianhe Pharmaceutical Co., Ltd. The main ingredient of the flagship product "T...

AI Technical Summary

Problems solved by technology

[0004] Over the years, the required medicinal materials have been obtained from wild resources. However, due to the vegetation degradation and artificial overharvesting and digging in the original place of the red euphorbia, the number of wild resources has decreased sharply, and the number of wild resources has a low seed setting rate and seed germination rate. The growth rate is slow, and wild medicinal materials are far from meeting people's needs. At present, the price has risen to 300 yuan per kilogram

[0005] As early as 1989, Pan Jichang introduced and cultivated by seeds and split-root propagation (Pan Jichang, Introduction and Cultivation Technique of Euphorbia euphorbia); after 1993, many researchers made great breakthroughs in the propagation of Euphorbia euphorbia by means of biotechnology (Huang euphorbia Hao, research on tissue culture of medicinal plant Euphorbia red bud; Ling Zhengzhu et al., Regenerated plants obtained from tissue culture of red Euphorbia; Chen Fangqing et al. Tissue culture of medicinal plant Euphorbia red bud; Qiu Anjing et al. tissue culture), but the transplanting and cultivation survival rate of tissue cultured seedlings is still a bottleneck that limits artificial planting, and the highest transplanting survival rate only reaches 88.36% (Huang Hao et al. Research)

[0006] At present, the tissue-cultured seedlings of Euphorbia euphorbia are similar to most other tissue-cultured seedlings. They are first transplanted into a loose substrate with ventilation and water retention for cultivation. After the tissue-cultured seedlings are fully adapted to the external atmospheric environment, they are then transplanted to the field for planting; This multi-transplantation planting method has little effect on the growth of medicinal plants with strong roots and buds, but it does not affect the growth of red euphorbia which is weak and can easily damage roots and buds during transplantation. Tissue culture seedlings have a greater impact, virtually reducing the survival rate of tissue culture seedlings

[0007] In addition, since the red euphorbia is a perennial medicinal plant, every winter, all the above-ground parts are downturned. In order to ensure the yield, it is often necessary to dig out the potato roots in winter during artificial planting, and bury them indoors or in a kiln in a well-ventilated, moist place. False planting in the sand or sawdust, and replanting the potato roots in the next year, the workload of planting management and maintenance is relatively large