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Application of bee pollen extract in preparation of blood pressure-lowering or liver-protecting medicines and health-care products

A technology for bee pollen and extract, applied in the biological field, can solve the problems of loss of active components of bee pollen, inability to reflect the omnipotent nutrient bank of bee pollen, etc., and achieve the effects of improving bioavailability and good application prospects

Inactive Publication Date: 2016-01-27
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the extracted components have good activity, other active components in bee pollen are lost during the extraction process, so that bee pollen only becomes a raw material for certain components, and cannot reflect the "universal nutrition bank" of bee pollen. features

Method used

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  • Application of bee pollen extract in preparation of blood pressure-lowering or liver-protecting medicines and health-care products
  • Application of bee pollen extract in preparation of blood pressure-lowering or liver-protecting medicines and health-care products
  • Application of bee pollen extract in preparation of blood pressure-lowering or liver-protecting medicines and health-care products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] One, the preparation method of bee pollen extract

[0022] Rapeseed pollen was used as raw material to prepare bee pollen extract according to the following process:

[0023] Take 1,000 grams of dried bee pollen, add 5L of ethanol with a volume concentration of 70%, and extract at 20°C for 10 hours. After the extraction is completed, filter and separate the extract from the solids for processing:

[0024] (1) The processing method of extract: the extract is concentrated and dried in vacuo, and the concentrated sample is dissolved with 20 milliliters of volume concentration 70% ethanol and then goes to the macroporous resin column, first rinses with deionized water of 5 times the column volume, and then uses The column was washed with 70% ethanol at a volume concentration of 3 times the column volume and fractions were collected, and the fraction washed with 70% ethanol was concentrated and dried in vacuo to obtain fraction 1.

[0025] (2) Solid matter treatment method:...

Embodiment 2

[0077] Using tea bee pollen as raw material, the bee pollen extract is prepared according to the following process:

[0078] Take 1,000 grams of dried tea bee pollen, add 50 L of ethyl acetate, and extract at 50°C for 2 hours. After the extraction is completed, filter and separate the extract from the solids for processing:

[0079] (1) The treatment method of the extract: the extract is concentrated and dried in vacuo, and the concentrated sample is dissolved in 50 milliliters of 70% ethanol and then put on a macroporous resin column. The column volume was washed with 70% ethanol and fractions were collected, and the fraction washed with 70% ethanol was concentrated and dried in vacuo to obtain fraction 1.

[0080] (2) Solid matter treatment method: solid matter is added 0.01M Tris-HCl buffer solution according to the ratio of raw material: solution=1kg:2L, and 3% bromelain of raw material mass is added, and enzymolysis is performed at 50°C for 1 hour; enzymolysis After comp...

Embodiment 3

[0084] Using corn bee pollen as raw material, the corn bee pollen extract is prepared according to the following process:

[0085] Take 1,000 grams of dried corn bee pollen, add 20 L of n-hexane, and extract at 30°C for 5 hours. After the extraction, filter and separate the extract from the solids for processing:

[0086] (1) The treatment method of the extract: the extract is concentrated and dried in vacuo, and the concentrated sample is dissolved in 30 ml of 70% ethanol and then put on a macroporous resin column, first rinsed with deionized water of 10 times the column volume, and then washed with 2 times of column volume The column volume was washed with 50% ethanol and the fractions were collected. The fractions washed with 50% ethanol were combined and then concentrated and dried in vacuo to obtain fraction 1.

[0087] (2) Solid matter treatment method: solid matter is added 0.05M phosphate buffer according to the ratio of raw material: solution = 1kg: 5L, then add 0.1% ...

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Abstract

The invention relates to a bee pollen extract, specifically relates to an application of the bee pollen extract in preparation of blood pressure-lowering and liver-protecting medicines and health-care products; the bee pollen extract as an active ingredient has the blood pressure-lowering and liver-protecting functions, can be made into various oral and externally-applied medicines or injections, and has a good application prospect in the fields of medicines and health-care products.

Description

technical field [0001] The invention relates to a bee pollen extract, and the bee pollen extract prepared by the invention has the functions of lowering blood pressure and protecting the liver. The technology belongs to the field of biotechnology. Background technique [0002] Bee pollen is the pollen grains collected by honey from flowering plants, which are formed by adding honey's own glandular secretions, saliva and nectar during the collection process. Bee pollen is one of the main sources of nutrition for the growth and reproduction of bee colonies, and has high nutritional value. Bee pollen contains protein, carbohydrates, flavonoids, trace elements, enzymes and other nutrients, so bee pollen has always been known as a "natural micro-nutrient bank". Studies on the biological activity of bee pollen have shown that bee pollen has the effects of improving cardiovascular disease, enhancing immunity, lowering blood sugar, anti-oxidation, anti-aging, and inhibiting prosta...

Claims

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Application Information

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IPC IPC(8): A61K35/64A61P9/12A61P1/16A23L1/29A23L1/076
Inventor 邹汉法靳艳
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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