A single spore propagation method for Puccinia maize

A multi-pump rust fungus and corn technology, which is applied in the methods of using spores, biochemical equipment and methods, fungi, etc., can solve the problems of high cost, inability to carry out, and limit a large number of sample research, and achieves high survival rate, Short time, the effect of shortening the breeding time

Inactive Publication Date: 2019-02-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, other studies are also limited by the lack of this method and cannot be carried out
There are also studies using single spores, but kits are required and the cost is high, which limits the research on a large number of samples
[0005] Many studies on maize southern rust need to be carried out based on pure strains. This fungus is a transsexual parasite that can only survive and reproduce on live corn plants. It is difficult to propagate monospora. Proliferation of Puccinia monospora

Method used

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  • A single spore propagation method for Puccinia maize
  • A single spore propagation method for Puccinia maize

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 is used to illustrate the method of the present invention

[0043] 1. Seedlings

[0044] 1) Choose a small flowerpot with a diameter of 100mm, mix the soil and substrate at a ratio of 1:1, and fill the flowerpot.

[0045] 2) Select plump seeds, soak the seeds with 0.1% hydrogen peroxide at room temperature for 48 hours, then pour off the water, cover the seeds with wet paper, induce germination in a ventilated place, and sow the seeds after the radicle grows about 2mm from the front of the seeds.

[0046] 3) Flatten the flower pot filled with soil with a flat-bottomed petri dish, soak in water for 10 minutes, and when the surface soil is slightly moist, use tweezers to pick 10 germinated seeds and put them in the flower pot. When planting, the radicle is facing upward and facing the flower pot center. Finally, cover the seeds with a layer of floating soil.

[0047] 2. Initial propagation of bacteria

[0048] 1) Inoculate the corn when it grows to three ...

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Abstract

The invention relates to a biological pathogen single-spore propagation method, in particular to a puccinia polysora underw single-spore propagation method which includes selecting single spore clusters of puccinia polysora underw on diseased leaves and vaccinating the single spore clusters to health corn seedlings, performing primary propagation, and transferring and spraying spore powder to new health corn seedlings to obtain propagated single-spore strain. Each of the health corn seedlings is of three leaves and one bud. The separation method has high success rate with short time, can be applied to propagation of strains in quality simultaneously, and is favorable for research on analysis based on the puccinia polysora underw single-spore strain.

Description

technical field [0001] The invention relates to a single-spore propagation method for biological pathogens, in particular to a single-spore propagation method for Puccinia maize. Background technique [0002] Puccinia maize can cause southern corn rust. It was first discovered in Hainan, my country in 1972. Since 2003, the disease has occurred in more than 20 provinces in my country. Therefore, the disease has been promoted from a secondary disease to a major disease. Corn has become the largest food crop in my country, so the control of southern corn rust is imminent. [0003] Chinese patent applications with publication numbers CN102146439A and CN102174647A respectively disclose the detection primers and molecular detection methods of the common type and the southern type corn rust pathogenic fungus, but do not disclose how to obtain the monospore propagation system of Puccinia maize. [0004] In recent years, maize southern rust has begun to attract everyone's attention....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N3/00
Inventor 马占鸿孙秋玉张克瑜王树和
Owner CHINA AGRI UNIV
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