A strain of Serratia nematophila and its application

A technology for Serratia and nematophilia, which is applied to the application field of Serratia nematophila and its growth-promoting properties, and can solve the problems of diazonium, light phosphorus and potassium deficiency, nutrient decline, nutritional imbalance and the like

Active Publication Date: 2018-06-29
CHINA TOBACCO HUNAN INDAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reason is that there is a lack of in-depth research on the correlation technology of fertilizer use efficiency, especially the control of fertilizer loss in southern tobacco areas and the nutrient absorption obstacles in northern tobacco areas have not been fundamentally solved; (2) the soil fertility of tobacco fields is different. There is a downward trend, due to the lack of good soil fertility measures, coupled with poor crop rotation conditions, continuous cropping in tobacco fields all year round, the main nutrients in the soil have a downward trend to varying degrees, and soil physical properties deteriorate; (3) Nutritional imbalance, heavy nitrogen Light phosphorus and potassium deficiency; (4) Lack of research on fertilization techniques for dry-farmed tobacco
However, there are no Serratia nematophila with efficient protein degradation and phosphorus-solubilizing functions at the same time, and there are no reports on its application in promoting tobacco growth.

Method used

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  • A strain of Serratia nematophila and its application
  • A strain of Serratia nematophila and its application
  • A strain of Serratia nematophila and its application

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with embodiment.

[0026] Serratia nematodiphila PR4 of the present invention, deposit unit: General Microbiology Center (CGMCC), China Microbiological Culture Collection Management Committee, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, deposit date: 2014 August 28, 2009, deposit number: CGMCC No.9614.

[0027] (1) Screening of CGMCC No.9614

[0028] Enrichment process in beef extract peptone liquid medium: Take tobacco rhizosphere soil collected from Hunan Sangzhi Guandiping and Liuyang Tobacco Institute, a total of 10 samples, numbered G1, G2, G3, G4, G5, L1 , L2, L3, L4, L5, each soil sample was weighed 10g, added to 100mL beef extract peptone liquid medium, 191r / min, 37°C shaker shaking culture for 24h.

[0029] The process of degrading protein with skimmed milk powder as the substrate: after taking the enriched culture solution and serially diluting it, from 10 -1 、10 ...

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Abstract

The invention discloses a strain of Serratia nematophila and its application. The strain is classified as Serratia nematodiphila PR4, depository unit: General Microbiology Center (CGMCC), China Committee for Microbial Culture Collection, address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, preserved Date: August 28, 2014, deposit number: CGMCC No.9614. The bacterial strain of the invention has good degradation effect on protein. The enzyme activity of the protease produced by the strain was determined to be 3.03IU by the Folinfen method. The strain has a good phosphorus-dissolving effect measured by the molybdenum blue colorimetric method. It indicated that the PR4 strain had potential application value in promoting the growth of tobacco.

Description

technical field [0001] The invention belongs to the technical field of plant rhizosphere growth-promoting bacteria and its application, and in particular relates to a serratia nematophila capable of promoting the growth of tobacco and having the functions of degrading protein and dissolving phosphorus and the application of its growth-promoting characteristics. Background technique [0002] Tobacco is an annual herbaceous plant belonging to the genus Nicotiana in the family Solanaceae. Likes temperature, likes light, tolerates drought, is afraid of waterlogging, tolerates barrenness, and needs more potassium. Tobacco leaves, stems, calyxes and fruits all have multicellular hairs, and the glandular hair cells contain chlorophyll, which can synthesize exudates containing gums and resins, which have a certain relationship with the aroma of tobacco. Tobacco petioles are inconspicuous or winged, with terminal panicles, tubular calyx, funnel-shaped corolla, and pink at the end. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/425
Inventor 易建华周东波周曙光彭宇王东蒲文宣汪耀富孙在军简永兴杜秉海丁延芹姚良同
Owner CHINA TOBACCO HUNAN INDAL CORP
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