Method for detecting beta-receptor agonists in pig urine

Abstract

The invention belongs to the field of analytical chemistry, and particularly relates to a method for detecting beta-receptor agonists in pig urine. According to the method for detecting beta-receptor agonists in pig urine, a pig urine sample is diluted through acetonitrile containing ammonia water and centrifuged after being evenly mixed with anhydrous sodium sulfate and sodium chloride, supernatant liquor is taken and extracted through a scattered solid phase, extracted liquid is subjected to high-speed centrifugal separation to precipitate, precipitate is dissolved in a methanol-0.1% formic acid aqueous solution, a microporous membrane is used for filtration, a liquid chromatogram-tandem mass spectrum is used for measuring, and an internal standard method is adopted for quantification. According to the method, the scattered solid phase is used for extraction, impurities are adsorbed, the sample is purified, but the beta-receptor agonists in the pig urine sample are not adsorbed, 26 beta-receptor agonists can be detected at a time, and flux is high. Meanwhile, scattered solid phase extraction one-step purification is adopted, the complex steps of leaching and eluting are avoided, operation is easy, an extraction agent is low in price, cost is lower than that of an existing solid phase extraction column purification method, the quantity of organic reagents in use is small, and the health of experimenters can be protected easily.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, in particular to a method for detecting beta-receptor agonists in pig urine, in particular to a method for simultaneously detecting 26 kinds of beta-receptor agonists in pig urine. Background technique [0002] β-agonists (β-agonists) are a class of chemically synthesized phenylethanolamines, including terbutaline, albuterol, zilpaterol, cimaterol, sibuterol, and hydroxyephedrine , Clenbuterol, Clenbuterol Bromo, Clenbuterol, Clorprenaline, Ractopamine, Tobuterol, Clenbuterol, Clenbuterol, Bromobuterol, Bambu Tributerol, Mabuterol, Profenbuterol, Mapenterol, Pembuterol, Phenylethanolamine A, Salmeterol, Clempanterol, Formoterol, Fenoterol, Isoprofen There are dozens of kinds of epinephrine, which can be divided into three categories: aniline type (such as clenbuterol), phenol type (such as albuterol) and diphenol type (such as terbutaline) with substituents. [0003] Adding β-receptor agonis...

AI Technical Summary

Problems solved by technology

The purification methods used in the pretreatment methods stipulated and reported in relevant national standards and technical literature are mostly mixed cation exchange (MCX) solid phase extraction and hydrophilic lipophilic balance (HLB) solid phase extraction. The purification principle of this method is adsorption-desorption, the operation steps are cumbersome, and a large amount of eluent and eluent need to be configured

In addition, the two types of solid phase extraction columns used in the purification method used in the pretreatment method of the current detection method are relatively expensive, and the cost is relatively high in the purification process of a large number of samples

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