Biological instrument pipeline cleaning solution and preparation method

A technology for biological instrument pipelines and cleaning fluids, applied to chemical instruments and methods, cleaning methods and utensils, detergent compositions, etc. Safety, overcoming damage, effective effect

Active Publication Date: 2019-01-29
HANGZHOU BEACONLAB BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With the rapid development of biotechnology, the application fields of biological instruments are becoming more and more extensive. However, biological instruments involving protein microfluidic control have relatively high precision requirements and are sensitive to pollutants. The attachment of residual protein will directly affect its accuracy and the effect of subsequent use
The small air bubbles remaining in the pipeline will affect the pressure balance inside the pipeline, thereby affecting the accuracy of the liquid; and the residual protein will pollute the subsequent use of the instrument, directly affecting the accuracy of the experimental results
[0003] Traditionally, there are two main types of cleaning fluids used to clean biological instrument pipelines: the first category is acid-base substances, which can denature residual proteins and lose their biological activity through the addition of acid-base substances and repeated washing, so as to avoid subsequent pipeline contamination. The impact of use on the experimental results, but this type of cleaning solution has several shortcomings: first, acid-base cleaning will cause corrosion to metal pipelines, will damage the smoothness of the inner surface of glass pipelines, and long-term use will also cause plastic The accelerated aging of the pipeline leads to poor sealing of the pipeline; secondly, although acid and alkali can deactivate biological protein molecules, they cannot be completely removed. The drainage is not smooth; third, after cleaning with acid and alkali, it is necessary to clean the residual acid and alkali with purified water. After the purified water is emptied, water droplets will remain on the inner surface of the pipeline due to surface tension. In subsequent use, Residual water droplets can dilute the protein concentration, resulting in inaccurate initial protein concentration in excretion, which in turn affects the experimental results
The second type is high-concentration surfactants. Although high-concentration surfactants can temporarily and effectively remove the bubbles generated in the pipeline and dissolve most of the remaining protein molecules, they are not suitable for stubborn protein adsorption (such as insoluble protein in water), the effect is not good

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Example 1: The mass percent content of each component of the new cleaning solution is: tris hydroxymethylaminomethane (Tris) as component I accounts for 0.5% to 1%, and β-cyclodextrin as component II accounts for 0.2% to 0.5% , ε-polylysine as component III accounts for 0.2% to 0.5%, sodium deoxycholate as component IV accounts for 0.5% to 1%, and purified water as solvent accounts for 97% to 98.6% as component V.

Embodiment 2

[0009] Example 2: On the basis of Example 1, trishydroxymethylaminomethane (Tris) accounts for 0.75% as component I, β-cyclodextrin accounts for 0.3% as component II, and ε-polylysine accounts for 0.3% as component III 0.25%, sodium deoxycholate as component IV accounted for 0.75%, purified water as a solvent as component V accounted for 97.95%.

Embodiment

[0010] Example 3: On the basis of Example 1, trishydroxymethylaminomethane (Tris) accounts for 0.5% as component I, β-cyclodextrin accounts for 0.4% as component II, and ε-polylysine as component III Accounting for 0.3%, sodium deoxycholate as component IV accounted for 0.8%, purified water as a solvent as component V accounted for 98%.

[0011] Example 4: On the basis of Example 1, trishydroxymethylaminomethane (Tris) accounts for 0.5% as component I, β-cyclodextrin accounts for 0.5% as component II, and ε-polylysine as component III Accounting for 0.2%, sodium deoxycholate as component IV accounted for 0.5%, purified water as a solvent as component V accounted for 98.3%.

[0012] Example 5: On the basis of the above examples, as described in claim 3, first weigh tris(Tris), β-cyclodextrin, ε-polylysine, sodium deoxycholate and mix , then add water to dissolve, and stir evenly, and distribute in airtight glass bottles.

[0013] Example 6: On the basis of Example 5, the meth...

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PUM

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Abstract

The invention relates to a biological instrument pipeline cleaning liquid capable of dissolving and removing protein molecules attached on a pipeline inner wall and a preparation method thereof. The biological instrument pipeline cleaning liquid comprises the components by the mass percentage: 0.5%-1% of tris(hydroxymethyl)aminomethane (Tris) as a component I, 0.2%-0.5% of beta-cyclodextrin as a component II, 0.2%-0.5% of epsilon-polylysine as a component III, 0.5%-1% of sodium deoxycholate as a component IV, and 97%-98.6% of purified water as a solvent and as a component V. The biological instrument pipeline cleaning liquid has the advantages: firstly, the cleaning liquid has obvious cleaning effect on protein residues in plastic, ceramic, metal and glass pipelines, the pipeline inner surface is not hung with water drops and has no liquid residues after cleaning, and influence cannot be caused on subsequent pipeline use; secondly, the cleaning liquid has no corrosion to the plastic, ceramic, metal and glass pipelines, and overcomes the damage on the pipeline inner wall through acid and alkali cleaning and other traditional methods; thirdly, the cleaning liquid is safe to use and harmless to human body surface, and has no operational risks; and fourthly, the cleaning liquid is not confined to cleaning of interiors of pipelines made of all kinds of materials, and also can be used for cleaning of various hard surfaces.

Description

technical field [0001] The invention relates to a biological instrument capable of dissolving and removing protein molecules attached to the inner wall of a pipeline, such as a coating machine used in an enzyme-linked immunosorbent assay, a gold spraying instrument used in the production of colloidal gold immunochromatography products, and a protein purification instrument. The pipeline cleaning solution and its preparation method belong to the field of biological instrument microfluidic control. Background technique [0002] With the rapid development of biotechnology, the application fields of biological instruments are becoming more and more extensive. However, biological instruments involving protein microfluidic control have relatively high precision requirements and are sensitive to pollutants. The attachment of residual protein will directly affect its accuracy and the effect of subsequent use. The tiny air bubbles remaining in the pipeline will affect the pressure b...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C11D7/60C11D7/32C11D7/26B08B9/032
CPCB08B9/032B08B2209/027C11D7/265C11D7/268C11D7/3209C11D7/3245
Inventor王国涛詹海燕余铭恩吴琼杉王璐朱伟项美华
OwnerHANGZHOU BEACONLAB BIOTECH CO LTD