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Printing method of biological chip and application of biological chip

A printing method and biochip technology, which can be used in organic compound libraries, tandem/parallel reactions, chemical libraries, etc., can solve problems such as the influence of membrane material and the influence of membrane on the detection performance of probe adsorption, immobilization, etc.

Active Publication Date: 2016-04-06
杨华卫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The problem of ink printing is that the ink itself contains a variety of complex physical and chemical substances, which may inhibit the proteins and enzymes used in protein chips and the color-developing enzymes used in gene chips; At this time, the high temperature generated by the printing equipment may have an impact on the membrane material, thereby affecting the adsorption and fixation of the membrane to the probe and its subsequent detection performance.

Method used

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  • Printing method of biological chip and application of biological chip
  • Printing method of biological chip and application of biological chip
  • Printing method of biological chip and application of biological chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Example 1: Preparation of bioprinted HPV gene chip by bioprinting method for detection of HPV16 samples

[0099] 1. Prepare bioprinting type HPV gene chip by bioprinting method of the present invention

[0100] (1) Preparation of marker solution and probe solution

[0101] Prepare hpv6 probe (positive control probe, marker), negative probe, HPV16 probe, hpv18 probe, hpv31 probe, hpv33 probe, hpv35 probe and hpv39 probe solution respectively, the concentration of probe solution is equal to is 2 μM.

[0102] (2) Preparation of bioprinted HPV gene chip using the above probe solution

[0103] Using the nitrocellulose membrane as the chip carrier, use the Boao PresonalAssayer16 spotting instrument to set the program, and print the hpv6 probe (positive control probe, marker) solution on the nitrocellulose membrane of the gene chip substrate into different divisions. At the same time, negative control probes and specific biological probes of the sample to be tested are prin...

Embodiment 2

[0116] Example 2: Preparation of bioprinted protein chip by bioprinting method for detecting TOX antibody samples

[0117] 1. Preparation of bioprinted protein chip by bioprinting method

[0118] (1) Preparation of marker solution and probe solution

[0119] Positive control probes (markers), negative control probes, purified antigen TOX probes, RV probes, CMV probes and HSV-II probe solutions were prepared respectively, and the concentrations of the probe solutions were all 2 μM.

[0120] (2) Preparation of bioprinted protein chip using the above probe solution

[0121] On the substrate of the protein chip, the positive control probes are printed as lines dividing different regions; at the same time, the negative control probes and the specific biological probes of the samples to be tested are printed on the same gene chip substrate in the form of dots , including purified antigen TOX probes, RV probes, CMV probes and HSV-II probes; the dots printed by these probes are pres...

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Abstract

The invention relates to a printing method of a biological chip and an application of the biological chip. The printing method comprises steps as follows: a to-be-detected sample and a marker or a to-be-detected sample, a marker and a biological molecule with a quality control function on the to-be-detected sample are fixed on a substrate of the biological chip in a graphic mode, and the biological chip is prepared. According to the biological chip prepared with the method, reactions of the chip cannot be inhibited, and the chip reacts very stably. With the adoption of the biological printing method, the preparation process of the chip is simplified, reaction results of the chip can be judged more quickly and more conveniently, besides, the detection throughput of the chip is increased, and accordingly, probes can be more accurately positioned on the chip.

Description

technical field [0001] The invention belongs to the technical field of preparation and application of biochips, and relates to a printing method for biochips and its application. Background technique [0002] Biochip technology is a high-throughput molecular biology detection technology for multi-index detection. Biochips include protein chips and gene chips. The matrix of the chip includes glass, porcelain, nylon membrane and nitrocellulose membrane, etc. Among them, chips based on glass substrates generally obtain chip detection signals through fluorescent labels; chips based on membrane substrates generally use enzymatic color development to interpret the color points at different positions on the chip by naked eyes or machines. Thereby, the detection result of the chip is obtained. [0003] A chip is also called a microarray, and each probe that makes up the array detects a different indicator. In order to achieve the purpose of multi-index detection, it is necessary...

Claims

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Application Information

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IPC IPC(8): C40B50/06C40B40/06C40B40/10
CPCB01J19/0046B01J2219/00608B01J2219/00637B01J2219/00722B01J2219/00725C40B40/06C40B40/10C40B50/06
Inventor 杨华卫曾冀
Owner 杨华卫
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