Analysis of Active Components in Kouyanqing and Construction Method of Fingerprint Feature Map

Abstract

The invention discloses an anti-inflammation active component group of a stomatitis clearing preparation and an analysis method. Fingerprint characteristic spectra of the component group are constructed and are applied to detecting the quality of the stomatitis clearing preparation. The analysis method which is a quality detecting method particularly includes detecting the quality of the stomatitis clearing preparation by the aid of a detection index which is anti-inflammation efficacy; uniformly designing difference samples with different component proportions; acquiring fingerprint characteristic data of the difference samples by the aid of UFLC-Q-TOF-MS / MS (ultrafast liquid chromatography-quadrupole-time of flight-mass spectrometry / mass spectrometry) technologies; acquiring anti-inflammation activity data of the preparation. The relevance between chemical components of the difference samples and the efficacy is comprehensively analyzed by the aid of the mathematical statistics method, accordingly, 29 anti-inflammation efficacy active components can be determined, and the fingerprint characteristic spectra of the component group can be constructed. Compared with the prior art, the anti-inflammation active component group and the analysis method have the advantages that the active component group is analyzed on the basis of the relevance between the spectra and the efficacy, accordingly, the quality of raw medicinal materials, semi-finished products and finished products can be directly, objectively, scientifically and comprehensively monitored in production procedures, and the effectiveness and the quality consistency of the products can be guaranteed.

Description

technical field [0001] The invention relates to the active component analysis of Kouyanqing preparation and its fingerprint spectrum construction and quality detection methods. Background technique [0002] The biological activity evaluation of traditional Chinese medicine is directly related to its safety and effectiveness. The effective material basis of traditional Chinese medicine is the chemical composition group closely related to biological activity in traditional Chinese medicine. It is the only way to promote the development and application of traditional Chinese medicine. As early as 2004, the US FDA's "Guidelines for the Development of Herbal Medicines" clearly pointed out that the research on herbal medicines should be based on the known ingredients and the research on drug effects, which reflects the importance of monitoring the bioactive ingredients of traditional Chinese medicines. , indicating that the research on bioactive components of traditional Chinese ...

AI Technical Summary

Problems solved by technology

The disadvantages of this fingerprint method are: (1) The chemical information and chemical interpretation provided by the fingerprint are less, a total of 23 common peaks were detected, and 7 known components were confirmed. ginseng and licorice, but the related characteristic components of Ophiopogon japonicus and Asparagus asparagus were not detected

(2) The biological activities of the chemical components corresponding to the characteristic peaks in the fingerprint spectrum are not clear, and the content of active components in Kouyanqing Granules cannot be effectively monitored, and the safety and effectiveness of the product cannot be ensured

[0005] In summary, there are still many deficiencies in the existing methods for constructing the fingerprints of Kouyanqing granules. On the basis of further improving its chemical information, it is necessary to focus on the core active ingredient groups and improve the quality detection methods.

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