The invention discloses a totally-enclosed nucleic acid extraction and detection integrated kit, which is characterized in that a plurality of areas are arranged in one kit, and the kit comprises a shell, a cleaning solution injector, an eluent injector and a PCR (Polymerase Chain Reaction) tube which are respectively filled with a lysis solution, a cleaning solution, an eluent and a reaction solution; when a sample is detected, the external push rod is pushed, a lysis solution mixed with the sample passes through the silicon material membrane, nucleic acid released by lysis of the sample is adsorbed by the silicon material membrane, along with pushing of the external push rod, the cleaning solution and the eluent are sequentially extruded out, and nucleic acid purification and elution arecompleted on the silicon material membrane. During elution, the shell integrally moves downwards with the help of external auxiliary equipment, the needle head penetrates into the closed PCR tube, and the external push rod is pushed to extrude purified nucleic acid to be mixed with reaction liquid. The PCR tube is subjected to temperature control and nucleic acid amplification reaction through the temperature circulating device, the optical detection equipment detects the amplification result, and totally-enclosed nucleic acid extraction detection is completed.