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Coronavirus and influenza virus detection device and method

A technology of influenza virus and coronavirus, applied in the direction of measuring devices, diseases resistant to media transmission, instruments, etc., can solve the problems of long detection time, low sensitivity, cumbersome operation, etc., achieve significant social and economic value, improve detection accuracy and The effect of detection efficiency

Active Publication Date: 2021-07-16
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of "complex operation, long detection time, and low sensitivity" in the current virus detection method in large-scale detection, the present invention provides a coronavirus and influenza virus detection device and method, the method is aimed at the detection of coronavirus and influenza virus The detection time is as short as 1 minute, and it can be used for the detection of single samples, mixed samples or mixed samples, and has potential social and economic value

Method used

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  • Coronavirus and influenza virus detection device and method
  • Coronavirus and influenza virus detection device and method
  • Coronavirus and influenza virus detection device and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] This embodiment provides the detection results of a single sample of the new coronavirus, which specifically includes the following steps:

[0076] Step 1. Prepare the novel coronavirus detection module 20. Among them, the processing steps of the transistor sensor array include the following seven steps:

[0077] (a) On a single-polished silicon oxide wafer (including the upper layer of 300nm SiO 2 and the lower 500 micron P-type doped Si) and then etch out the electrode pattern after spin-coating photoresist;

[0078] (b) metal electrodes (5 nanometers of chromium and 50 nanometers of gold) are evaporated at the electrode pattern, and the electrodes include a source and a drain, wherein the source is the current input terminal and the drain is the current output terminal;

[0079] (c) 8 wt.% polymethyl methacrylate (PMMA) was spin-coated on the graphene grown on the metal substrate, and the graphene / PMMA thin film was transferred to Si / SiO with metal electrodes by el...

Embodiment 2

[0096] Detect according to the method described in the specific embodiment, the difference between this embodiment and embodiment 1 is:

[0097] (1) The samples to be tested used in this example were collected in accordance with the "Technical Specifications for 10-in-1 Mixed Detection of New Coronavirus Nucleic Acid" issued by the National Health and Medical Commission, and were directly added to the sample pool of the virus detection device without pretreatment. Among them, M1 is a mixed sample of pharyngeal swabs from 10 healthy people; M3 is the addition of 5 picomoles per liter (5×10 –12 mol / L) of the new coronavirus spike protein "simulated positive mixed sample";

[0098] (2) Add 100 microliters of negative quality control material (virus preservation solution) to a sample pool of the detection module, and set the output voltage of the virus detection device so that the fluctuation range of the electrical signal response of the transistor sensor combination is less than...

Embodiment 3

[0101] Detect according to the method described in the specific embodiment, the difference between this embodiment and embodiment 1 is:

[0102] (1) The sample to be tested used in this embodiment is a mixed sample, which is directly added to the sample pool of the virus detection device without pretreatment. Among them, M1 is a mixed sample of throat swabs from 10 healthy people; M2, M3, M4, M5 and M6 are "simulated positive mixed samples" obtained by adding a certain concentration of the new coronavirus spike protein to M1. After calibration, M2 contains 0.5 picomoles per liter (0.5×10 –12 mol / L) of the new coronavirus spike protein; M3 contains 5 picomoles per liter (5 × 10 –12 mol / L) of the novel coronavirus spike protein; M4 contains 50 picomoles per liter (50×10 –12 mol / L) of the novel coronavirus spike protein; M5 contains 500 picomoles per liter (500×10 –12 mol / L) of the novel coronavirus spike protein; M6 contains 5 nanomoles per liter (50×10 –9 mol / L) of the new ...

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Abstract

The invention belongs to the technical field of biological detection and particularly discloses a coronavirus and influenza virus detection device and method. The device comprises a detection module, a signal processing circuit, a controller, a displayer, a digital-to-analog conversion circuit and a clock, wherein the detection module is a sample cell and a transistor sensor combination which is integrated in the sample cell and is used for measuring different targets, and the detection module can comprise a sample cell array, so a plurality of to-be-detected samples can be detected at the same time. The detection method comprises the following steps of adding a to-be-detected sample into the sample pool, and reading the electric signal response of each transistor sensor in the sample pool to judge whether the to-be-detected sample contains the to-be-detected virus or not. According to the method, the shortest detection time for the coronavirus and the influenza virus is 1 minute, and the method can be used for detecting a single-person sample, a mixed sample or a mixed sample and has potential social and economic values.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a coronavirus and influenza virus detection device and method. Background technique [0002] The new coronavirus and most influenza viruses are RNA (ribonucleic acid) viruses, which mutate quickly and have strong transmission capabilities. During the peak season of influenza epidemic, the superimposed effect of novel coronavirus and influenza virus increases the difficulty of detection. At present, the detection methods of new coronavirus and influenza virus are mainly nucleic acid detection and kit detection: nucleic acid detection is aimed at nucleocapsid protein gene, envelope protein gene or open reading frame gene of new coronavirus, which needs to be extracted, amplified, detected and Interpretation and other steps, nucleic acid detection takes a long time (at least 2 hours), requires high equipment and operators, and even misdiagnosed results such...

Claims

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Application Information

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IPC IPC(8): G01N27/414G01N27/327
CPCG01N27/4145G01N27/4146G01N27/3278Y02A50/30G01N33/5438G01N33/56983G01N2333/11G01N2333/165
Inventor 魏大程戴长昊曹伴鹏王学军
Owner FUDAN UNIV
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