Totally-enclosed nucleic acid extraction and detection integrated kit

A fully enclosed, kit-based technology, applied in biochemical instruments, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems of unfavorable gene detection technology popularization, time-consuming, complicated procedures, etc., to improve detection accuracy and detection Efficiency, reduced operational difficulty, and reduced complexity

Active Publication Date: 2020-08-18
青岛英赛特生物科技有限公司 +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the genetic testing procedures are complex and require professionals to conduct testing in the laboratory, which is not conducive to the popularization of genetic testing technology
During genetic testi

Method used

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  • Totally-enclosed nucleic acid extraction and detection integrated kit

Examples

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Example Embodiment

[0023] Example 1:

[0024] Such as figure 1 As shown, a fully enclosed nucleic acid extraction and detection integrated kit includes a main body assembly, a combined syringe assembly 6, a collection assembly and several PCR tubes 15;

[0025] Wherein, the main body assembly includes a housing 3, a housing cover 2, an external push rod 1, a silicon membrane 10, and a needle 12. The housing 3 is a cylinder with an open top and a closed bottom. A housing cover 2 is threadedly connected to the top, the housing cover 2 penetrates up and down, the housing 3 and the housing cover 2 are movably fitted with an external push rod 1, and the inner bottom of the housing 3 is provided with a silicon film 10 , The bottom of the housing 3 is connected with a needle 12;

[0026] The combined syringe assembly 6 is located in the housing 3 and includes a cleaning fluid injector 8, an eluent injector 7, and a support rod 9. The cleaning fluid injector 8 and the eluent injector 7 are placed side by sid...

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Abstract

The invention discloses a totally-enclosed nucleic acid extraction and detection integrated kit, which is characterized in that a plurality of areas are arranged in one kit, and the kit comprises a shell, a cleaning solution injector, an eluent injector and a PCR (Polymerase Chain Reaction) tube which are respectively filled with a lysis solution, a cleaning solution, an eluent and a reaction solution; when a sample is detected, the external push rod is pushed, a lysis solution mixed with the sample passes through the silicon material membrane, nucleic acid released by lysis of the sample is adsorbed by the silicon material membrane, along with pushing of the external push rod, the cleaning solution and the eluent are sequentially extruded out, and nucleic acid purification and elution arecompleted on the silicon material membrane. During elution, the shell integrally moves downwards with the help of external auxiliary equipment, the needle head penetrates into the closed PCR tube, and the external push rod is pushed to extrude purified nucleic acid to be mixed with reaction liquid. The PCR tube is subjected to temperature control and nucleic acid amplification reaction through the temperature circulating device, the optical detection equipment detects the amplification result, and totally-enclosed nucleic acid extraction detection is completed.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and in particular relates to a fully enclosed nucleic acid extraction and detection integrated kit. Background technique [0002] Genetic testing is a technology for detecting DNA through blood, other body fluids, or cells. It is to take the peripheral venous blood or other tissue cells of the tested person, amplify their genetic information, and use specific equipment to detect the DNA molecules in the tested person's cells. It is a method to detect and analyze the gene types and gene defects contained in it and whether its expression function is normal, so that people can understand their own genetic information, clarify the cause of disease or predict the risk of a certain disease in the body. [0003] Genetic testing can also be used for virus detection, and virus infection can be judged by detecting the presence or absence of viral nucleic acid. Viruses can be divided into DNA viruse...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/24C12M1/00
CPCC12N15/1003C12Q1/6806C12Q1/686
Inventor 李贵阳李彦王熊曲心怡王振华杨中兴郑军贵汪琼沈巍
Owner 青岛英赛特生物科技有限公司
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