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Method for extracting tricin from corn bracts

A technology of alfalfa and corn, which is applied in the field of biomedicine, can solve the problems of low extraction efficiency of alfalfa, achieve the effects of increasing the burden of impurity removal, reducing impurity content, and shortening the extraction cycle

Active Publication Date: 2016-04-20
杭州研谱科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The invention provides a method for extracting alfalfa from corn bracts, which solves the problem of low extraction efficiency of alfalfa in the prior art

Method used

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  • Method for extracting tricin from corn bracts
  • Method for extracting tricin from corn bracts
  • Method for extracting tricin from corn bracts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] A method of extracting alfalfa from corn bracts in this embodiment comprises the following steps:

[0059] (1) Weigh 10 kg of dried corn bract pieces (particle size less than 0.5 cm), put them into a 200 L extraction tank, add 100 L of water, soak for 3 hours under stirring conditions, discard the extraction solution, and extract the slag for subsequent use;

[0060] (2) Extract the extraction slag with a volume fraction of 80% ethanol solution added with hydrochloric acid (12mol / L) to reflux, and concentrate the obtained extract under reduced pressure to obtain a concentrate; wherein, the amount of hydrochloric acid added is the corn bract 0.03% of crushed mass (ie 0.003kg);

[0061] Specifically: ① Add 100 L of ethanol solution with a volume fraction of 80% to the extraction residue, and start stirring;

[0062] ② Turn on the jacket steam, heat until ethanol reflux, start timing, and reflux extraction for 1 hour;

[0063] ③ After the reflux extraction is completed, ...

Embodiment 2

[0081] A method of extracting alfalfa from corn bracts in this embodiment comprises the following steps:

[0082] (1) Weigh 300 kg of dried corn bract pieces (particle size less than 0.5 cm), put them into a 3000L extraction tank, add 2400L of water, soak for 3h under stirring conditions, discard the extract, and extract the slag for subsequent use;

[0083] (2) Extract the extraction slag with a volume fraction of 85% ethanol solution added with hydrochloric acid (12mol / L) to reflux, and concentrate the resulting extract under reduced pressure to obtain a concentrate; wherein the amount of hydrochloric acid added is corn bract 0.03% of crushed mass (ie 0.09kg);

[0084] Specifically: ①Add 2400L of ethanol solution with a volume fraction of 85% to the extraction residue, and start stirring;

[0085] ② Turn on the jacket steam, heat until ethanol reflux, start timing, and reflux extraction for 1 hour;

[0086] ③ After the reflux extraction is completed, the feed liquid is pas...

Embodiment 3

[0097] A method of extracting alfalfa from corn bracts in this embodiment comprises the following steps:

[0098](1) Weigh 200 kg of dried corn bract pieces (particle size less than 0.5 cm), put them into a 3000L extraction tank, add 2000L of water, soak for 3h under stirring conditions, discard the extract, and extract the slag for subsequent use;

[0099] (2) Extract the extraction slag with a volume fraction of 90% ethanol solution added with hydrochloric acid (12mol / L) to reflux, and concentrate the resulting extract under reduced pressure to obtain a concentrate; wherein the amount of hydrochloric acid added is corn bract 0.03% of crushed mass (ie 0.06kg);

[0100] Specifically: ①Add 2000L of the above-mentioned ethanol solution to the extraction residue, and start stirring;

[0101] ② Turn on the jacket steam and heat until ethanol refluxes, start timing, and reflux for 2 hours;

[0102] ③After the reflux extraction is completed, pass the feed liquid through a 400-mesh...

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Abstract

The invention discloses a method for extracting tricin from corn bracts. The method includes the steps that the corn bracts are subjected to normal temperature extraction with water, and extraction residues are obtained; an ethanol solution with the volume fraction of 80% to 90% is used for conducting reflux extraction on the extraction residues, an acid medium is added to the ethanol solution, an obtained extracting solution is subjected to vacuum concentration, and concentrate is obtained; the concentrate is diluted with water, the pH value is adjusted to 7.8 to 8.2, and supernatant is taken after solid-liquid separation; macroreticular resin column chromatography is conducted on the supernatant, and corn bract extract containing tricin is obtained. Normal temperature extraction is firstly conducted with water during alcohol extraction, and therefore protein, polysaccharide and other impurities can be removed in advance. The acid medium is added to the ethanol solution used for reflux extraction, and therefore the extraction efficiency of low-polarity tricin can be effectively improved. The pH value of the concentrate diluent is adjusted to 7.8 to 8.2, at the moment, chlorophyll, protein, polysaccharide and other impurities in the concentrate diluent quickly settle in the weak base environment, and the extraction period of tricin is greatly shortened.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for extracting alfalfa from corn bracts. Background technique [0002] Alfalfa (tricin) is also known as wheat flavin, wheat flavonoids. Alfalfa is yellow needle crystal (acetic acid-water), yellow powder (chloroform-methanol), melting point 291-292°C. Alfalfa is present in the stems and leaves of grasses Miscanthus sinensis Anderss., leguminous alfalfa (Medicagosativa L.), Compositae and other plants. Alfalfa has a relaxing effect on the isolated guinea pig intestine, has a mild antioxidant effect, can prevent the oxidation of adrenaline, and has a mild estrogen-like effect, and can also be used as a dye intermediate. However, most of the alfalfa-related products currently on the market are prepared from alfalfa. [0003] For example, the Chinese invention patent application with the application publication number CN102911146A discloses a method for extracting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/899C07D311/30C07D311/40
CPCA61K36/899A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55C07D311/30C07D311/40
Inventor 黄六仔
Owner 杭州研谱科技有限公司
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