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SSR molecular marker linked with wheat spikelet number QTL and application of SSR molecular marker

A technology of molecular markers and spikelet number, which is applied in the field of molecular biology and crop genetics and breeding, can solve the problem of not many molecular markers, achieve high linkage degree and improve the efficiency of breeding work

Inactive Publication Date: 2016-04-27
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, currently there are not many tightly linked molecular markers related to the trait of spikelet number in wheat that can be used in practical molecular breeding.

Method used

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  • SSR molecular marker linked with wheat spikelet number QTL and application of SSR molecular marker
  • SSR molecular marker linked with wheat spikelet number QTL and application of SSR molecular marker
  • SSR molecular marker linked with wheat spikelet number QTL and application of SSR molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Acquisition of wheat spikelet number QTLQSns.sau.3D and its molecular marker gdm8

[0031] 1. The female parent of Tibetan semi-wild wheat Q1028 was crossed with the wheat variety Zhengmai 9023 as the male parent to obtain the hybrid F1. F1 was self-crossed to obtain F2, and 186 individual plants were randomly selected in F2 for self-crossing. Harvest a single plant, continue selfing in the next year, and repeat this until the F8 generation, and obtain recombinant inbred lines containing 186 lines to form a genetic mapping population.

[0032] 2. Phenotypic identification of spikelet number characteristics of recombinant inbred lines

[0033] The number of spikelets of recombinant inbred lines was analyzed and identified at the maturity stage of wheat.

[0034] 3. SSR analysis and DArT analysis

[0035] a) DNA extraction: the DNA of parental Q1028, Zhengmai 9023 and recombinant inbred lines population plants was extracted by CTAB method.

[0036]b) DArT ana...

Embodiment 2

[0047] Example 2 Application of molecular marker gdm8 in identifying spikelet number QTLQSns.sau-3D wheat plants

[0048] 1. Using Tibetan semi-wild wheat Q1028 as the female parent and common wheat line 99E18 as the male parent to construct a recombinant inbred line, and randomly select 8 lines from the offspring lines.

[0049] 2. Carry out gdm8 marker detection to the obtained 8 strains, the specific method is: extract the DNA of 8 strains at the seedling stage; use it as a template, and utilize the primers (SEQIDNo: 1 and 2) of amplifying the molecular marker gdm8 Perform PCR amplification.

[0050] PCR amplification system: 5μL 10×PCR reaction buffer, 1.5UExTaq DNA polymerase, 2mMMgCl 2 , 0.2mMdNTP, 150ng each of upstream and downstream primers, 100ng template DNA, and double-distilled water to a total of 50μL.

[0051] PCR program: pre-denaturation at 94°C for 5 minutes; denaturation at 94°C for 30 s, annealing at 55°C for 45 s, extension at 72°C for 30 s, a total of 3...

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Abstract

The invention provides an SSR molecular marker linked with wheat spikelet number QTL and application of the SSR molecular marker. The molecular marker gdm8 linked with the wheat spikelet number, disclosed by the invention, locates on a wheat 3D chromosome, and the molecular marker is a flanking marker (co-dominant marker) of spikelet number QTL QSns.sau.3D on the long arm of the wheat 3D chromosome, with high linkage degree. The marker can be used for detecting the spikelet number QTL on the wheat 3D chromosome and for rapidly screening out plants having the site, so that molecular assisted selection for the high production of wheat can be conveniently performed. The molecular marker gdm8 provided by the invention, which is closely linked with the spikelet number QTL QSns.sau.3D on the wheat 3D, is applicable to wheat spikelet number character mapping, so that the plants with less spikelet are discarded during breeding; therefore, the molecular marker can improve the working efficiency of breeding and provide a basis for researches on the wheat spikelet number gene.

Description

technical field [0001] The invention relates to the fields of molecular biology and crop genetic breeding, in particular to the SSR molecular markers linked to the wheat spikelet number QTL and the application thereof. Background technique [0002] Wheat is an important food crop in my country, with an annual area of ​​more than 20 million hectares, accounting for about 27% of the area of ​​food crops; the total output is more than 100 million tons, accounting for about 22% of the output of food crops. Wheat yield is composed of the number of ears per unit area, the number of grains per ear, and the weight of grains. The number of grains per spike is the most active factor among the yield components, and it can be adjusted to a large extent. The number of grains per spike is determined by the number of spikelets, the number of differentiated florets, and the seed setting rate of florets. The number of spikelets is the prerequisite for the formation of florets, and the incre...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 马建兰秀锦孙敏罗伟郑有良魏育明江千涛陈国跃刘亚西祁鹏飞
Owner SICHUAN AGRI UNIV
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