A kind of avian influenza virus detection kit

A bird flu virus and detection kit technology, applied in the field of medical detection, can solve problems such as complicated operation requirements, infectivity, and difficulty in popularization, and achieve the effect of enhanced color rendering and rapid detection
CN105548541BInactive Publication Date: 2017-11-07王好玉
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
王好玉
Publication Date
2017-11-07
Estimated Expiration
Not applicable · inactive patent
Patent Text Reader

Abstract

The invention discloses a bird flu virus detection reagent kit which comprises a bird flu virus cyst membrane antigen, a nanogold-labeled rabbit anti-chicken IgY antibody detection probe, and a dot immunogold concentrated diafiltration device. The concentrated diafiltration device is composed of a small plastic box, a nitrocellulose membrane where the bird flu virus cyst membrane antigen is added dropwise, and a water-absorbing pad. The water-absorbing pad is placed in a groove of the small plastic box, and the nitrocellulose membrane is placed between the water-absorbing pad and a kit cover. On the basis that a bird flu virus cyst membrane has the antigen characteristic, the nitrocellulose membrane of the bird flu virus cyst membrane antigen is prepared, then a nanogold-labeled rabbit anti-chicken IgY antibody serves as the detection probe, the dot immunogold concentrated diafiltration method is used for detection, detection speed is high, sensitivity is high, and the whole detection reagent kit is easy to prepare and low in cost.
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Description

technical field

[0001] The invention relates to the technical field of medical detection, in particular to an avian influenza virus detection kit. Background technique

[0002] Avian influenza is an acute infectious disease of poultry caused by avian influenza virus, and some subtypes of avian influenza virus can also infect humans. Avian influenza viruses can be divided into 16 HA subtypes and 9 NA subtypes according to the differences in the antigenic relationship between their surface glycoproteins hemagglutinin (HA) and neuraminidase (NA). According to its pathogenicity, it can be divided into highly pathogenic avian influenza and low pathogenic avian influenza. Highly pathogenic avian influenza includes H5 and H7 subtypes. Especially in recent years, the H5 subtype avian influenza outbreaks have caused huge economic losses to the poultry industry in many countries and regions.

[0003] At present, the methods for detecting avian influenza virus mainly include: fluores...

Claims

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